Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis
In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-03-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200014&lng=en&tlng=en |
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| author | Patrícia Longuinhos Peixoto Evaldo Nascimento Guilherme Grossi Lopes Cançado Rodrigo Rodrigues Cambraia de Miranda Regina Lunardi Rocha Ricardo Nascimento Araújo Ricardo Toshio Fujiwara |
| author_facet | Patrícia Longuinhos Peixoto Evaldo Nascimento Guilherme Grossi Lopes Cançado Rodrigo Rodrigues Cambraia de Miranda Regina Lunardi Rocha Ricardo Nascimento Araújo Ricardo Toshio Fujiwara |
| author_sort | Patrícia Longuinhos Peixoto |
| collection | DOAJ |
| description | In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we found that the crude extract of the parasite presented limited sensitivity, specificity and high cross-reactivity against other parasites, we identified 42, 58, 68 and 97-kDa semi-purified antigens as the most promising candidates for immunodiagnosis. Moreover, the 58 and 68-kDa antigens presented the lowest IgM cross-reactivity. When tested as a combination, a mixture of the 58 and 68-kDa antigens presented 100% sensitivity and specificity, as well as minor cross-reactivity. Although the combination of the 42, 58, 68 and 97-kDa antigens presented 100% sensitivity at a dilution of 1:40, the low specificity and high cross-reactivity observed suggested a limited use for diagnostic purposes. Our data suggested that the 58 and 68-kDa antigens might be most suitable for the immunodiagnosis of human toxocariasis. |
| first_indexed | 2024-03-12T04:59:46Z |
| format | Article |
| id | doaj.art-75509462a4464240bf378edc5c460398 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:59:46Z |
| publishDate | 2011-03-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-75509462a4464240bf378edc5c4603982023-09-03T09:14:11ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-03-01106220020610.1590/S0074-02762011000200014S0074-02762011000200014Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasisPatrícia Longuinhos Peixoto0Evaldo Nascimento1Guilherme Grossi Lopes Cançado2Rodrigo Rodrigues Cambraia de Miranda3Regina Lunardi Rocha4Ricardo Nascimento Araújo5Ricardo Toshio Fujiwara6Instituto de Ciências BiológicasSanta Casa de Misericórdia de Belo HorizonteInstituto de Ciências BiológicasInstituto de Ciências BiológicasUniversidade Federal de Minas GeraisInstituto de Ciências BiológicasInstituto de Ciências BiológicasIn the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we found that the crude extract of the parasite presented limited sensitivity, specificity and high cross-reactivity against other parasites, we identified 42, 58, 68 and 97-kDa semi-purified antigens as the most promising candidates for immunodiagnosis. Moreover, the 58 and 68-kDa antigens presented the lowest IgM cross-reactivity. When tested as a combination, a mixture of the 58 and 68-kDa antigens presented 100% sensitivity and specificity, as well as minor cross-reactivity. Although the combination of the 42, 58, 68 and 97-kDa antigens presented 100% sensitivity at a dilution of 1:40, the low specificity and high cross-reactivity observed suggested a limited use for diagnostic purposes. Our data suggested that the 58 and 68-kDa antigens might be most suitable for the immunodiagnosis of human toxocariasis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200014&lng=en&tlng=enToxocaraELISAvisceral larva migrans |
| spellingShingle | Patrícia Longuinhos Peixoto Evaldo Nascimento Guilherme Grossi Lopes Cançado Rodrigo Rodrigues Cambraia de Miranda Regina Lunardi Rocha Ricardo Nascimento Araújo Ricardo Toshio Fujiwara Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis Memorias do Instituto Oswaldo Cruz Toxocara ELISA visceral larva migrans |
| title | Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis |
| title_full | Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis |
| title_fullStr | Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis |
| title_full_unstemmed | Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis |
| title_short | Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis |
| title_sort | identification of candidate antigens from adult stages of toxocara canis for the serodiagnosis of human toxocariasis |
| topic | Toxocara ELISA visceral larva migrans |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000200014&lng=en&tlng=en |
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