Qualitative and Quantitative Saponin Contents in Five Sea Cucumbers from the Indian Ocean
To avoid predation, holothuroids produce feeding-deterrent molecules in their body wall and viscera, the so-called saponins. Five tropical sea cucumber species of the family Holothuriidae were investigated in order to study their saponin content in two different organs, the body wall and the Cuvieri...
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| Format: | Article |
| Language: | English |
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MDPI AG
2010-01-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/8/1/173/ |
| _version_ | 1798037656535302144 |
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| author | Patrick Flammang Pascal Gerbaux Séverine Van Dyck |
| author_facet | Patrick Flammang Pascal Gerbaux Séverine Van Dyck |
| author_sort | Patrick Flammang |
| collection | DOAJ |
| description | To avoid predation, holothuroids produce feeding-deterrent molecules in their body wall and viscera, the so-called saponins. Five tropical sea cucumber species of the family Holothuriidae were investigated in order to study their saponin content in two different organs, the body wall and the Cuvierian tubules. Mass spectrometry techniques (MALDI- and ESI-MS) were used to detect and analyze saponins. The smallest number of saponins was observed in Holothuria atra, which contained a total of four congeners, followed by Holothuria leucospilota, Pearsonothuria graeffei and Actinopyga echinites with six, eight and ten congeners, respectively. Bohadschia subrubra revealed the highest saponin diversity (19 congeners). Saponin mixtures also varied between the two body compartments within a given animal. A semi-quantitative approach completed these results and showed that a high diversity of saponins is not particularly correlated to a high saponin concentration. Although the complexity of the saponin mixtures described makes the elucidation of their respective biological roles difficult, the comparisons between species and between body compartments give some clues about how these molecules may act as predator repellents. |
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| format | Article |
| id | doaj.art-7551b9f83b5e41f8913ffaa49f8f9e83 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T21:29:31Z |
| publishDate | 2010-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-7551b9f83b5e41f8913ffaa49f8f9e832022-12-22T04:02:12ZengMDPI AGMarine Drugs1660-33972010-01-018117318910.3390/md8010173Qualitative and Quantitative Saponin Contents in Five Sea Cucumbers from the Indian OceanPatrick FlammangPascal GerbauxSéverine Van DyckTo avoid predation, holothuroids produce feeding-deterrent molecules in their body wall and viscera, the so-called saponins. Five tropical sea cucumber species of the family Holothuriidae were investigated in order to study their saponin content in two different organs, the body wall and the Cuvierian tubules. Mass spectrometry techniques (MALDI- and ESI-MS) were used to detect and analyze saponins. The smallest number of saponins was observed in Holothuria atra, which contained a total of four congeners, followed by Holothuria leucospilota, Pearsonothuria graeffei and Actinopyga echinites with six, eight and ten congeners, respectively. Bohadschia subrubra revealed the highest saponin diversity (19 congeners). Saponin mixtures also varied between the two body compartments within a given animal. A semi-quantitative approach completed these results and showed that a high diversity of saponins is not particularly correlated to a high saponin concentration. Although the complexity of the saponin mixtures described makes the elucidation of their respective biological roles difficult, the comparisons between species and between body compartments give some clues about how these molecules may act as predator repellents.http://www.mdpi.com/1660-3397/8/1/173/Holothuriidaetriterpene glycosidestandem mass spectrometryhemolysisorcinol reaction |
| spellingShingle | Patrick Flammang Pascal Gerbaux Séverine Van Dyck Qualitative and Quantitative Saponin Contents in Five Sea Cucumbers from the Indian Ocean Marine Drugs Holothuriidae triterpene glycosides tandem mass spectrometry hemolysis orcinol reaction |
| title | Qualitative and Quantitative Saponin Contents in Five Sea Cucumbers from the Indian Ocean |
| title_full | Qualitative and Quantitative Saponin Contents in Five Sea Cucumbers from the Indian Ocean |
| title_fullStr | Qualitative and Quantitative Saponin Contents in Five Sea Cucumbers from the Indian Ocean |
| title_full_unstemmed | Qualitative and Quantitative Saponin Contents in Five Sea Cucumbers from the Indian Ocean |
| title_short | Qualitative and Quantitative Saponin Contents in Five Sea Cucumbers from the Indian Ocean |
| title_sort | qualitative and quantitative saponin contents in five sea cucumbers from the indian ocean |
| topic | Holothuriidae triterpene glycosides tandem mass spectrometry hemolysis orcinol reaction |
| url | http://www.mdpi.com/1660-3397/8/1/173/ |
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