Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens
Coffee endophytes have been studied for almost 74 years, and several studies have demonstrated coffee-endophytic fungi with antibacterial and antifungal potential for human and plant pathogens. In this study, we isolated and identified a total of 235 strains of endophytic fungi from coffee leaf tiss...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/8/7/698 |
| _version_ | 1797405905409540096 |
|---|---|
| author | Li Lu Samantha C. Karunarathna Kevin D. Hyde Nakarin Suwannarach Abdallah M. Elgorban Steven L. Stephenson Salim Al-Rejaie Ruvishika S. Jayawardena Saowaluck Tibpromma |
| author_facet | Li Lu Samantha C. Karunarathna Kevin D. Hyde Nakarin Suwannarach Abdallah M. Elgorban Steven L. Stephenson Salim Al-Rejaie Ruvishika S. Jayawardena Saowaluck Tibpromma |
| author_sort | Li Lu |
| collection | DOAJ |
| description | Coffee endophytes have been studied for almost 74 years, and several studies have demonstrated coffee-endophytic fungi with antibacterial and antifungal potential for human and plant pathogens. In this study, we isolated and identified a total of 235 strains of endophytic fungi from coffee leaf tissues collected in four coffee plantations in Pu’er city, Yunnan province, China. Molecular identification was carried out using maximum likelihood phylogenetic analysis of nuclear ribosomal internal transcribed spacer (ITS1-5.8S rDNA-ITS2) sequences, while the colonization rate and the isolation frequency were also calculated. Two pathogenic fungi (<i>Alternaria alternata</i> and <i>Penicillium digitatum</i>) and two pathogenic bacteria (<i>Pseudomonas syringae</i> and <i>Salmonella enterica</i> subsp. <i>enterica</i>) were used for screening the antagonistic activities of 61 strains of coffee-endophytic fungi by a dual-culture test assay while maximum likelihood phylogenetic analysis confirmed their natural classification. This is the first study of coffee-leaf-endophytic fungal diversity in China, and the results revealed that coffee-endophytic fungi from this study belong to the Ascomycota, distributed among two classes, 10 orders, and 17 families. Concurrently, endophytic fungi isolates distributed in <i>Arthrinium</i>, <i>Biscogniauxia</i>, <i>Daldinia</i>, <i>Diaporthe,</i> and <i>Nigrospora</i> showed strong antagonistic activities against the pathogens. For the pathogens <i>Alternaria alternata</i> and <i>Pseudomonas syringae</i>, <i>Nigrospora</i> XCE-7 showed the best inhibitory effects with inhibition rates of 71.76% and 61.11%, respectively. For the pathogen <i>Penicillium digitatum</i>, <i>Daldinia</i> ME-9 showed the best inhibitory effect with a 74.67% inhibition rate, while <i>Biscogniauxia</i> PTE-7 and <i>Daldinia</i> T5E-1-3 showed the best inhibitory effect with a rate of 60.42% against the pathogen <i>Salmonella enterica</i> subsp. <i>enterica</i>. Overall, our study shows the diversity of coffee endophytes in four coffee-growing areas in Pu’er city, Yunnan province, China, and their potential use as biological control agents against two fungal and two bacterial pathogens. |
| first_indexed | 2024-03-09T03:17:59Z |
| format | Article |
| id | doaj.art-7553a6e8759a44409ff60461045a89c3 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T03:17:59Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-7553a6e8759a44409ff60461045a89c32023-12-03T15:15:47ZengMDPI AGJournal of Fungi2309-608X2022-06-018769810.3390/jof8070698Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial PathogensLi Lu0Samantha C. Karunarathna1Kevin D. Hyde2Nakarin Suwannarach3Abdallah M. Elgorban4Steven L. Stephenson5Salim Al-Rejaie6Ruvishika S. Jayawardena7Saowaluck Tibpromma8Center for Yunnan Plateau Biological Resources Protection and Utilization, Yunnan Engineering Research Center of Fruit Wine, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011, ChinaCenter for Yunnan Plateau Biological Resources Protection and Utilization, Yunnan Engineering Research Center of Fruit Wine, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011, ChinaCenter of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, ThailandResearch Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh P.O. Box 145111, Saudi ArabiaDepartment of Biological Sciences, University of Arkansas, Fayetteville, AR 72701, USADepartment of Pharmacology & Toxicology, College of Pharmacy, King Saud University, Riyadh P.O. Box 145111, Saudi ArabiaCenter of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, ThailandCenter for Yunnan Plateau Biological Resources Protection and Utilization, Yunnan Engineering Research Center of Fruit Wine, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011, ChinaCoffee endophytes have been studied for almost 74 years, and several studies have demonstrated coffee-endophytic fungi with antibacterial and antifungal potential for human and plant pathogens. In this study, we isolated and identified a total of 235 strains of endophytic fungi from coffee leaf tissues collected in four coffee plantations in Pu’er city, Yunnan province, China. Molecular identification was carried out using maximum likelihood phylogenetic analysis of nuclear ribosomal internal transcribed spacer (ITS1-5.8S rDNA-ITS2) sequences, while the colonization rate and the isolation frequency were also calculated. Two pathogenic fungi (<i>Alternaria alternata</i> and <i>Penicillium digitatum</i>) and two pathogenic bacteria (<i>Pseudomonas syringae</i> and <i>Salmonella enterica</i> subsp. <i>enterica</i>) were used for screening the antagonistic activities of 61 strains of coffee-endophytic fungi by a dual-culture test assay while maximum likelihood phylogenetic analysis confirmed their natural classification. This is the first study of coffee-leaf-endophytic fungal diversity in China, and the results revealed that coffee-endophytic fungi from this study belong to the Ascomycota, distributed among two classes, 10 orders, and 17 families. Concurrently, endophytic fungi isolates distributed in <i>Arthrinium</i>, <i>Biscogniauxia</i>, <i>Daldinia</i>, <i>Diaporthe,</i> and <i>Nigrospora</i> showed strong antagonistic activities against the pathogens. For the pathogens <i>Alternaria alternata</i> and <i>Pseudomonas syringae</i>, <i>Nigrospora</i> XCE-7 showed the best inhibitory effects with inhibition rates of 71.76% and 61.11%, respectively. For the pathogen <i>Penicillium digitatum</i>, <i>Daldinia</i> ME-9 showed the best inhibitory effect with a 74.67% inhibition rate, while <i>Biscogniauxia</i> PTE-7 and <i>Daldinia</i> T5E-1-3 showed the best inhibitory effect with a rate of 60.42% against the pathogen <i>Salmonella enterica</i> subsp. <i>enterica</i>. Overall, our study shows the diversity of coffee endophytes in four coffee-growing areas in Pu’er city, Yunnan province, China, and their potential use as biological control agents against two fungal and two bacterial pathogens.https://www.mdpi.com/2309-608X/8/7/698biocontrol agents<i>Coffea</i>coffee-endophytic fungipathogenic fungipathogenic bacteria |
| spellingShingle | Li Lu Samantha C. Karunarathna Kevin D. Hyde Nakarin Suwannarach Abdallah M. Elgorban Steven L. Stephenson Salim Al-Rejaie Ruvishika S. Jayawardena Saowaluck Tibpromma Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens Journal of Fungi biocontrol agents <i>Coffea</i> coffee-endophytic fungi pathogenic fungi pathogenic bacteria |
| title | Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens |
| title_full | Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens |
| title_fullStr | Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens |
| title_full_unstemmed | Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens |
| title_short | Endophytic Fungi Associated with Coffee Leaves in China Exhibited In Vitro Antagonism against Fungal and Bacterial Pathogens |
| title_sort | endophytic fungi associated with coffee leaves in china exhibited in vitro antagonism against fungal and bacterial pathogens |
| topic | biocontrol agents <i>Coffea</i> coffee-endophytic fungi pathogenic fungi pathogenic bacteria |
| url | https://www.mdpi.com/2309-608X/8/7/698 |
| work_keys_str_mv | AT lilu endophyticfungiassociatedwithcoffeeleavesinchinaexhibitedinvitroantagonismagainstfungalandbacterialpathogens AT samanthackarunarathna endophyticfungiassociatedwithcoffeeleavesinchinaexhibitedinvitroantagonismagainstfungalandbacterialpathogens AT kevindhyde endophyticfungiassociatedwithcoffeeleavesinchinaexhibitedinvitroantagonismagainstfungalandbacterialpathogens AT nakarinsuwannarach endophyticfungiassociatedwithcoffeeleavesinchinaexhibitedinvitroantagonismagainstfungalandbacterialpathogens AT abdallahmelgorban endophyticfungiassociatedwithcoffeeleavesinchinaexhibitedinvitroantagonismagainstfungalandbacterialpathogens AT stevenlstephenson endophyticfungiassociatedwithcoffeeleavesinchinaexhibitedinvitroantagonismagainstfungalandbacterialpathogens AT salimalrejaie endophyticfungiassociatedwithcoffeeleavesinchinaexhibitedinvitroantagonismagainstfungalandbacterialpathogens AT ruvishikasjayawardena endophyticfungiassociatedwithcoffeeleavesinchinaexhibitedinvitroantagonismagainstfungalandbacterialpathogens AT saowalucktibpromma endophyticfungiassociatedwithcoffeeleavesinchinaexhibitedinvitroantagonismagainstfungalandbacterialpathogens |