Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum
Fusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR) in cereals worldwide. Since F. culmorum has a great level of genetic diversity and the parasexual stage is assumed for this phytopathogen, characterization of isolates from different regions is significant step in...
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2018-02-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1423 |
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| author | Işıl Melis Zümrüt Berna Tunalı Bayram Kansu Gülşen Uz Emre Yörük Fatih Ölmez Ayşegül Sarıkaya |
| author_facet | Işıl Melis Zümrüt Berna Tunalı Bayram Kansu Gülşen Uz Emre Yörük Fatih Ölmez Ayşegül Sarıkaya |
| author_sort | Işıl Melis Zümrüt |
| collection | DOAJ |
| description | Fusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR) in cereals worldwide. Since F. culmorum has a great level of genetic diversity and the parasexual stage is assumed for this phytopathogen, characterization of isolates from different regions is significant step in food safety and controlling the HB. In this study, it was aimed to characterize totally 37 F. culmorum isolates from Turkey via sequence related amplified polymorphism (SRAP) marker based genotyping. MAT-1/MAT-2 type assay was also used in order to reveal intraspecific variation in F. culmorum. MAT-1 and MAT-2 specific primer pairs for mating assays resulted in 210 and 260 bp bands, respectively. 11 of isolates were belonged to MAT-1 type whereas 19 samples were of MAT-2. Remaining 7 samples yielded both amplicons. Totally 9 SRAP primer sets yielded amplicons from all isolates. Genetic similarity values were ranged from 39 to 94.7%. Total band number was 127 and PCR product sizes were in the range of 0.1-2.5 kb. Amplicon numbers for individuals were ranged from 1 to 16. According to data obtained from current study, SRAP based genotyping is powerful tool for supporting the data obtained from investigations including phenotypic and agro-ecological characteristics. Findings showed that SRAP-based markers could be useful in F. culmorum characterization. |
| first_indexed | 2024-04-10T13:53:16Z |
| format | Article |
| id | doaj.art-755c7a2f6d4a46e39e95f24cdcef580c |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T13:53:16Z |
| publishDate | 2018-02-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-755c7a2f6d4a46e39e95f24cdcef580c2023-02-15T16:10:35ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2018-02-016214514910.24925/turjaf.v6i2.145-149.1423781Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorumIşıl Melis Zümrüt0Berna Tunalı1Bayram Kansu2Gülşen Uz3Emre Yörük4Fatih Ölmez5Ayşegül Sarıkaya6Department of Molecular Biology and Genetics/Faculty of Arts and Science, Yeni Yüzyil University, 34034 İstanbulDepartment of Plant Pathology/Faculty of Agriculture, Ondokuz Mayıs University, 55100 SamsunAnimal and Plant Production Department/Vocational School, Ondokuz Mayıs University, 55100 SamsunDepartment of Molecular Biology and Genetics/Faculty of Arts and Science, Yeni Yüzyil University, 34034 İstanbulDepartment of Molecular Biology and Genetics/Faculty of Arts and Science, Yeni Yüzyil University, 34034 İstanbulBiotechnology Department/Field Crop Central Research Institute, 06170 AnkaraDepartment of Medical Biology and Genetics/Medical Faculty, Yeni Yüzyıl University, 34034 İstanbulFusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR) in cereals worldwide. Since F. culmorum has a great level of genetic diversity and the parasexual stage is assumed for this phytopathogen, characterization of isolates from different regions is significant step in food safety and controlling the HB. In this study, it was aimed to characterize totally 37 F. culmorum isolates from Turkey via sequence related amplified polymorphism (SRAP) marker based genotyping. MAT-1/MAT-2 type assay was also used in order to reveal intraspecific variation in F. culmorum. MAT-1 and MAT-2 specific primer pairs for mating assays resulted in 210 and 260 bp bands, respectively. 11 of isolates were belonged to MAT-1 type whereas 19 samples were of MAT-2. Remaining 7 samples yielded both amplicons. Totally 9 SRAP primer sets yielded amplicons from all isolates. Genetic similarity values were ranged from 39 to 94.7%. Total band number was 127 and PCR product sizes were in the range of 0.1-2.5 kb. Amplicon numbers for individuals were ranged from 1 to 16. According to data obtained from current study, SRAP based genotyping is powerful tool for supporting the data obtained from investigations including phenotypic and agro-ecological characteristics. Findings showed that SRAP-based markers could be useful in F. culmorum characterization.http://www.agrifoodscience.com/index.php/TURJAF/article/view/1423Fusarium culmorumgenetic diversityMAT-1 |
| spellingShingle | Işıl Melis Zümrüt Berna Tunalı Bayram Kansu Gülşen Uz Emre Yörük Fatih Ölmez Ayşegül Sarıkaya Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum Turkish Journal of Agriculture: Food Science and Technology Fusarium culmorum genetic diversity MAT-1 |
| title | Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum |
| title_full | Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum |
| title_fullStr | Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum |
| title_full_unstemmed | Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum |
| title_short | Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium culmorum |
| title_sort | genotyping via sequence related amplified polymorphism markers in fusarium culmorum |
| topic | Fusarium culmorum genetic diversity MAT-1 |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1423 |
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