Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic Fibrosis
The IL-13Rα1 signaling pathway and M2 macrophages play crucial roles in schistosome egg-induced hepatic fibrosis via the expression of pro-fibrotic molecules. This study aims to investigate the inhibitory effect and mechanism of action of corilagin on schistosome egg-induced hepatic fibrosis via the...
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| Format: | Article |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00443/full |
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| author | Yi-Qing Li Yun-Fei Chen Yi-Ping Dang Yao Wang Zhen-Zhong Shang Qian Ma Yu-Jie Wang Juan Zhang Lei Luo Quan-Qiang Li Lei Zhao |
| author_facet | Yi-Qing Li Yun-Fei Chen Yi-Ping Dang Yao Wang Zhen-Zhong Shang Qian Ma Yu-Jie Wang Juan Zhang Lei Luo Quan-Qiang Li Lei Zhao |
| author_sort | Yi-Qing Li |
| collection | DOAJ |
| description | The IL-13Rα1 signaling pathway and M2 macrophages play crucial roles in schistosome egg-induced hepatic fibrosis via the expression of pro-fibrotic molecules. This study aims to investigate the inhibitory effect and mechanism of action of corilagin on schistosome egg-induced hepatic fibrosis via the IL-13Rα1 signaling pathway in M2 macrophages in vitro and in vivo. The mRNA and protein expression of IL-13Rα1, PPARγ, KLF4, SOCS1, STAT6, p-STAT6, and TGF-β was measured in vitro with corilagin treatment after IL-13 stimulation and in vivo corilagin treatment after effectively killing the adult schistosomes in schistosome-infected mice. Histological analysis of liver tissue was assessed for the degree of hepatic fibrosis. The results revealed that corilagin significantly reduced the expression of PPARγ, KLF4, SOCS1, p-STAT6, and TGF-β compared with model group and praziquantel administration (p < 0.01 or p < 0.05) in vivo and in vitro, which indicated a strong inhibitory effect of corilagin on IL-13Rα1 signaling pathway. As well, the inhibitory effect of corilagin showed a significant dose-dependence (p < 0.05). The area of fibrosis and distribution of M2 macrophages in mouse liver tissue were reduced significantly and dose-dependently with corilagin treatment compared to model group or praziquantel administration (p < 0.01 or p < 0.05), indicating that corilagin suppressed IL-13Rα1 signaling pathway and M2 macrophage polarization effectively in vivo. Furthermore, the anti-fibrogenic effect persisted even when IL-13Rα1 was up- or down-regulated in vitro. In conclusion, corilagin can suppress schistosome egg-induced hepatic fibrosis via inhibition of M2 macrophage polarization in the IL-13Rα1 signaling pathway. |
| first_indexed | 2024-04-14T02:31:08Z |
| format | Article |
| id | doaj.art-755ca6611d3847f79b9ac9d8da088459 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-14T02:31:08Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-755ca6611d3847f79b9ac9d8da0884592022-12-22T02:17:40ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882017-10-01710.3389/fcimb.2017.00443291440Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic FibrosisYi-Qing Li0Yun-Fei Chen1Yi-Ping Dang2Yao Wang3Zhen-Zhong Shang4Qian Ma5Yu-Jie Wang6Juan Zhang7Lei Luo8Quan-Qiang Li9Lei Zhao10Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSchool of Clinical Medicine, Hubei University of Chinese Medicine, Wuhan, ChinaSchool of Basic Medical Sciences, Guangxi University of Chinese Medicine, Nanning, ChinaSchool of Life Science, Hubei University, Wuhan, ChinaDepartment of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaSchool of Clinical Medicine, Hubei University of Chinese Medicine, Wuhan, ChinaSchool of Clinical Medicine, Hubei University of Chinese Medicine, Wuhan, ChinaSchool of Clinical Medicine, Guangxi University of Chinese Medicine, Nanning, ChinaDepartment of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaThe IL-13Rα1 signaling pathway and M2 macrophages play crucial roles in schistosome egg-induced hepatic fibrosis via the expression of pro-fibrotic molecules. This study aims to investigate the inhibitory effect and mechanism of action of corilagin on schistosome egg-induced hepatic fibrosis via the IL-13Rα1 signaling pathway in M2 macrophages in vitro and in vivo. The mRNA and protein expression of IL-13Rα1, PPARγ, KLF4, SOCS1, STAT6, p-STAT6, and TGF-β was measured in vitro with corilagin treatment after IL-13 stimulation and in vivo corilagin treatment after effectively killing the adult schistosomes in schistosome-infected mice. Histological analysis of liver tissue was assessed for the degree of hepatic fibrosis. The results revealed that corilagin significantly reduced the expression of PPARγ, KLF4, SOCS1, p-STAT6, and TGF-β compared with model group and praziquantel administration (p < 0.01 or p < 0.05) in vivo and in vitro, which indicated a strong inhibitory effect of corilagin on IL-13Rα1 signaling pathway. As well, the inhibitory effect of corilagin showed a significant dose-dependence (p < 0.05). The area of fibrosis and distribution of M2 macrophages in mouse liver tissue were reduced significantly and dose-dependently with corilagin treatment compared to model group or praziquantel administration (p < 0.01 or p < 0.05), indicating that corilagin suppressed IL-13Rα1 signaling pathway and M2 macrophage polarization effectively in vivo. Furthermore, the anti-fibrogenic effect persisted even when IL-13Rα1 was up- or down-regulated in vitro. In conclusion, corilagin can suppress schistosome egg-induced hepatic fibrosis via inhibition of M2 macrophage polarization in the IL-13Rα1 signaling pathway.http://journal.frontiersin.org/article/10.3389/fcimb.2017.00443/fullcorilaginliver fibrosisschistosome eggIL-13Rα1M2 macrophage |
| spellingShingle | Yi-Qing Li Yun-Fei Chen Yi-Ping Dang Yao Wang Zhen-Zhong Shang Qian Ma Yu-Jie Wang Juan Zhang Lei Luo Quan-Qiang Li Lei Zhao Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic Fibrosis Frontiers in Cellular and Infection Microbiology corilagin liver fibrosis schistosome egg IL-13Rα1 M2 macrophage |
| title | Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic Fibrosis |
| title_full | Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic Fibrosis |
| title_fullStr | Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic Fibrosis |
| title_full_unstemmed | Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic Fibrosis |
| title_short | Corilagin Counteracts IL-13Rα1 Signaling Pathway in Macrophages to Mitigate Schistosome Egg-Induced Hepatic Fibrosis |
| title_sort | corilagin counteracts il 13rα1 signaling pathway in macrophages to mitigate schistosome egg induced hepatic fibrosis |
| topic | corilagin liver fibrosis schistosome egg IL-13Rα1 M2 macrophage |
| url | http://journal.frontiersin.org/article/10.3389/fcimb.2017.00443/full |
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