Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID‐19
Abstract Aims We aimed to assess whether expression of whole‐blood RNA of sodium proton exchanger 1 (NHE1) and glucose transporter 1 (GLUT1) is associated with COVID‐19 infection and outcome in patients presenting to the emergency department with respiratory infections. Furthermore, we investigated...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-02-01
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| Series: | ESC Heart Failure |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ehf2.13063 |
| _version_ | 1818329931244896256 |
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| author | Julian Mustroph Julian Hupf Frank Hanses Katja Evert Maria J. Baier Matthias Evert Christine Meindl Stefan Wagner Ute Hubauer Gabriela Pietrzyk Simon Leininger Stephan Staudner Manuel Vogel Stefan Wallner Markus Zimmermann Samuel Sossalla Lars S. Maier Carsten Jungbauer |
| author_facet | Julian Mustroph Julian Hupf Frank Hanses Katja Evert Maria J. Baier Matthias Evert Christine Meindl Stefan Wagner Ute Hubauer Gabriela Pietrzyk Simon Leininger Stephan Staudner Manuel Vogel Stefan Wallner Markus Zimmermann Samuel Sossalla Lars S. Maier Carsten Jungbauer |
| author_sort | Julian Mustroph |
| collection | DOAJ |
| description | Abstract Aims We aimed to assess whether expression of whole‐blood RNA of sodium proton exchanger 1 (NHE1) and glucose transporter 1 (GLUT1) is associated with COVID‐19 infection and outcome in patients presenting to the emergency department with respiratory infections. Furthermore, we investigated NHE1 and GLUT1 expression in the myocardium of deceased COVID‐19 patients. Methods and results Whole‐blood quantitative assessment of NHE1 and GLUT1 RNA was performed using quantitative PCR in patients with respiratory infection upon first contact in the emergency department and subsequently stratified by SARS‐CoV‐2 infection status. Assessment of NHE1 and GLUT1 RNA using PCR was also performed in left ventricular myocardium of deceased COVID‐19 patients. NHE1 expression is up‐regulated in whole blood of patients with COVID‐19 compared with other respiratory infections at first medical contact in the emergency department (control: 0.0021 ± 0.0002, COVID‐19: 0.0031 ± 0.0003, P = 0.01). The ratio of GLUT1 to NHE1 is significantly decreased in the blood of COVID‐19 patients who are subsequently intubated and/or die (severe disease) compared with patients with moderate disease (moderate disease: 0.497 ± 0.083 vs. severe disease: 0.294 ± 0.0336, P = 0.036). This ratio is even further decreased in the myocardium of patients who deceased from COVID‐19 in comparison with the myocardium of non‐infected donors. Conclusions NHE1 and GLUT1 may be critically involved in the disease progression of SARS‐CoV‐2 infection. We show here that SARS‐CoV‐2 infection critically disturbs ion channel expression in the heart. A decreased ratio of GLUT1/NHE1 could potentially serve as a biomarker for disease severity in patients with COVID‐19. |
| first_indexed | 2024-12-13T12:55:54Z |
| format | Article |
| id | doaj.art-755dac0791aa4cb894fc8e6b52253364 |
| institution | Directory Open Access Journal |
| issn | 2055-5822 |
| language | English |
| last_indexed | 2024-12-13T12:55:54Z |
| publishDate | 2021-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | ESC Heart Failure |
| spelling | doaj.art-755dac0791aa4cb894fc8e6b522533642022-12-21T23:45:11ZengWileyESC Heart Failure2055-58222021-02-018130931610.1002/ehf2.13063Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID‐19Julian Mustroph0Julian Hupf1Frank Hanses2Katja Evert3Maria J. Baier4Matthias Evert5Christine Meindl6Stefan Wagner7Ute Hubauer8Gabriela Pietrzyk9Simon Leininger10Stephan Staudner11Manuel Vogel12Stefan Wallner13Markus Zimmermann14Samuel Sossalla15Lars S. Maier16Carsten Jungbauer17Department of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyEmergency Department University Hospital Regensburg Regensburg GermanyEmergency Department University Hospital Regensburg Regensburg GermanyInstitute of Pathology University Hospital Regensburg Regensburg GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyInstitute of Pathology University Hospital Regensburg Regensburg GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyDepartment of Clinical Chemistry and Laboratory Medicine University Hospital Regensburg Regensburg GermanyEmergency Department University Hospital Regensburg Regensburg GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyDepartment of Internal Medicine II (Cardiology) University Hospital Regensburg Franz‐Josef‐Strauß‐Allee 11 Regensburg 93053 GermanyAbstract Aims We aimed to assess whether expression of whole‐blood RNA of sodium proton exchanger 1 (NHE1) and glucose transporter 1 (GLUT1) is associated with COVID‐19 infection and outcome in patients presenting to the emergency department with respiratory infections. Furthermore, we investigated NHE1 and GLUT1 expression in the myocardium of deceased COVID‐19 patients. Methods and results Whole‐blood quantitative assessment of NHE1 and GLUT1 RNA was performed using quantitative PCR in patients with respiratory infection upon first contact in the emergency department and subsequently stratified by SARS‐CoV‐2 infection status. Assessment of NHE1 and GLUT1 RNA using PCR was also performed in left ventricular myocardium of deceased COVID‐19 patients. NHE1 expression is up‐regulated in whole blood of patients with COVID‐19 compared with other respiratory infections at first medical contact in the emergency department (control: 0.0021 ± 0.0002, COVID‐19: 0.0031 ± 0.0003, P = 0.01). The ratio of GLUT1 to NHE1 is significantly decreased in the blood of COVID‐19 patients who are subsequently intubated and/or die (severe disease) compared with patients with moderate disease (moderate disease: 0.497 ± 0.083 vs. severe disease: 0.294 ± 0.0336, P = 0.036). This ratio is even further decreased in the myocardium of patients who deceased from COVID‐19 in comparison with the myocardium of non‐infected donors. Conclusions NHE1 and GLUT1 may be critically involved in the disease progression of SARS‐CoV‐2 infection. We show here that SARS‐CoV‐2 infection critically disturbs ion channel expression in the heart. A decreased ratio of GLUT1/NHE1 could potentially serve as a biomarker for disease severity in patients with COVID‐19.https://doi.org/10.1002/ehf2.13063COVID‐19InfectionHeartGLUT1NHE1 |
| spellingShingle | Julian Mustroph Julian Hupf Frank Hanses Katja Evert Maria J. Baier Matthias Evert Christine Meindl Stefan Wagner Ute Hubauer Gabriela Pietrzyk Simon Leininger Stephan Staudner Manuel Vogel Stefan Wallner Markus Zimmermann Samuel Sossalla Lars S. Maier Carsten Jungbauer Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID‐19 ESC Heart Failure COVID‐19 Infection Heart GLUT1 NHE1 |
| title | Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID‐19 |
| title_full | Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID‐19 |
| title_fullStr | Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID‐19 |
| title_full_unstemmed | Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID‐19 |
| title_short | Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID‐19 |
| title_sort | decreased glut1 nhe1 rna expression in whole blood predicts disease severity in patients with covid 19 |
| topic | COVID‐19 Infection Heart GLUT1 NHE1 |
| url | https://doi.org/10.1002/ehf2.13063 |
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