<i>BocODD1</i> and <i>BocODD2</i> Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale

<i>BocODD1</i> and <i>BocODD2</i> Regulate the Biosynthesis of Progoitrin Glucosinolate in Chinese Kale

Progoitrin (2-hydroxy-3-butenyl glucosinolate, PRO) is the main source of bitterness of <i>Brassica</i> plants. Research on the biosynthesis of PRO glucosinolate can aid the understanding of the nutritional value in <i>Brassica</i> plants. In this study, four ODD genes likely...

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Bibliographic Details
Main Authors: Shuanghua Wu, Ting Zhang, Yudan Wang, Muxi Chen, Jianguo Yang, Fei Li, Ying Deng, Zhangsheng Zhu, Jianjun Lei, Guoju Chen, Bihao Cao, Changming Chen
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:International Journal of Molecular Sciences
Subjects:
progoitrin glucosinolate
ODD
biosynthesis of glucosinolates
gene function
Chinese kale
Online Access:https://www.mdpi.com/1422-0067/23/23/14781
Description
Summary:Progoitrin (2-hydroxy-3-butenyl glucosinolate, PRO) is the main source of bitterness of <i>Brassica</i> plants. Research on the biosynthesis of PRO glucosinolate can aid the understanding of the nutritional value in <i>Brassica</i> plants. In this study, four ODD genes likely involved in PRO biosynthesis were cloned from Chinese kale. These four genes, designated as <i>BocODD1–4</i>, shared 75–82% similarities with the <i>ODD</i> sequence of <i>Arabidopsis</i>. The sequences of these four <i>BocODD</i>s were analyzed, and <i>BocODD1</i> and <i>BocODD2</i> were chosen for further study. The gene <i>BocODD1,2</i> showed the highest expression levels in the roots, followed by the leaves, flowers, and stems, which is in accordance with the trend of the PRO content in the same tissues. Both the expression levels of <i>BocODD1,2</i> and the content of PRO were significantly induced by high- and low-temperature treatments. The function of <i>BocODDs</i> involved in PRO biosynthesis was identified. Compared with the wild type, the content of PRO was increased twofold in the over-expressing <i>BocODD1</i> or <i>BocODD2</i> plants. Meanwhile, the content of PRO was decreased in the <i>BocODD1</i> or <i>BocODD2</i> RNAi lines more than twofold compared to the wildtype plants. These results suggested that <i>BocODD1</i> and <i>BocODD2</i> may play important roles in the biosynthesis of PRO glucosinolate in Chinese kale.
ISSN:1661-6596
1422-0067

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