Olive-Leaf Extracts Modulate Inflammation and Oxidative Stress Associated with Human <i>H. pylori</i> Infection
<i>Helicobacter pylori</i> (<i>H. pylori</i>) is one of the major human pathogens and the main cause of pathological damages that can progress from chronic gastritis to gastric cancer. During the colonization of gastric mucosa, this bacterium provokes a strong inflammatory re...
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MDPI AG
2021-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/12/2030 |
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| author | Jose Manuel Silvan Esperanza Guerrero-Hurtado Alba Gutiérrez-Docio Teresa Alarcón-Cavero Marin Prodanov Adolfo J. Martinez-Rodriguez |
| author_facet | Jose Manuel Silvan Esperanza Guerrero-Hurtado Alba Gutiérrez-Docio Teresa Alarcón-Cavero Marin Prodanov Adolfo J. Martinez-Rodriguez |
| author_sort | Jose Manuel Silvan |
| collection | DOAJ |
| description | <i>Helicobacter pylori</i> (<i>H. pylori</i>) is one of the major human pathogens and the main cause of pathological damages that can progress from chronic gastritis to gastric cancer. During the colonization of gastric mucosa, this bacterium provokes a strong inflammatory response and subsequent oxidative process, which are associated with tissue damage. Therefore, the objective of this research was to evaluate the ability of two olive-leaf extracts (E1 and E2) to modulate the inflammatory response and oxidative stress in <i>H. pylori</i>-infected human gastric AGS cells. The obtained results showed that both extracts significantly decreased interleukin-8 (IL-8) secretion and reactive oxygen species (ROS) production in human gastric AGS cells. Both extracts also showed antibacterial activity against different <i>H. pylori</i> strains. HPLC-PAD-MS characterization demonstrated that extract E1 was mainly composed of highly hydrophilic compounds, such as hydroxytyrosol (HT) and its glucosides, and it was the most effective extract as an antibacterial agent. In contrast, extract E2 was composed mostly of moderately hydrophilic compounds, such as oleuropein (OLE), and it was more effective than extract E1 as an anti-inflammatory agent. Both extracts exhibited similar potential to decrease ROS production. These results show the importance of standardizing the extract composition according to the bioactive properties that should be potentiated. |
| first_indexed | 2024-03-10T04:38:37Z |
| format | Article |
| id | doaj.art-75612cd706fd4c6ab88f7f823ed61ccc |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T04:38:37Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-75612cd706fd4c6ab88f7f823ed61ccc2023-11-23T03:34:37ZengMDPI AGAntioxidants2076-39212021-12-011012203010.3390/antiox10122030Olive-Leaf Extracts Modulate Inflammation and Oxidative Stress Associated with Human <i>H. pylori</i> InfectionJose Manuel Silvan0Esperanza Guerrero-Hurtado1Alba Gutiérrez-Docio2Teresa Alarcón-Cavero3Marin Prodanov4Adolfo J. Martinez-Rodriguez5Microbiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), Cantoblanco Campus, Autonomous University of Madrid, C/Nicolás Cabrera 9, 28049 Madrid, SpainDepartment of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CEI, CSIC-UAM), Cantoblanco Campus, Autonomous University of Madrid, C/Nicolás Cabrera 9, 28049 Madrid, SpainDepartment of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CEI, CSIC-UAM), Cantoblanco Campus, Autonomous University of Madrid, C/Nicolás Cabrera 9, 28049 Madrid, SpainMicrobiology Department, Hospital Universitario de La Princesa, 28006 Madrid, SpainDepartment of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CEI, CSIC-UAM), Cantoblanco Campus, Autonomous University of Madrid, C/Nicolás Cabrera 9, 28049 Madrid, SpainMicrobiology and Food Biocatalysis Group (MICROBIO), Department of Biotechnology and Food Microbiology, Institute of Food Science Research (CIAL, CSIC-UAM), Cantoblanco Campus, Autonomous University of Madrid, C/Nicolás Cabrera 9, 28049 Madrid, Spain<i>Helicobacter pylori</i> (<i>H. pylori</i>) is one of the major human pathogens and the main cause of pathological damages that can progress from chronic gastritis to gastric cancer. During the colonization of gastric mucosa, this bacterium provokes a strong inflammatory response and subsequent oxidative process, which are associated with tissue damage. Therefore, the objective of this research was to evaluate the ability of two olive-leaf extracts (E1 and E2) to modulate the inflammatory response and oxidative stress in <i>H. pylori</i>-infected human gastric AGS cells. The obtained results showed that both extracts significantly decreased interleukin-8 (IL-8) secretion and reactive oxygen species (ROS) production in human gastric AGS cells. Both extracts also showed antibacterial activity against different <i>H. pylori</i> strains. HPLC-PAD-MS characterization demonstrated that extract E1 was mainly composed of highly hydrophilic compounds, such as hydroxytyrosol (HT) and its glucosides, and it was the most effective extract as an antibacterial agent. In contrast, extract E2 was composed mostly of moderately hydrophilic compounds, such as oleuropein (OLE), and it was more effective than extract E1 as an anti-inflammatory agent. Both extracts exhibited similar potential to decrease ROS production. These results show the importance of standardizing the extract composition according to the bioactive properties that should be potentiated.https://www.mdpi.com/2076-3921/10/12/2030olive-leaf extract<i>Helicobacter pylori</i>antioxidant activityanti-inflammatory activityHPLC-PAD-MS characterizationhydroxytyrosol |
| spellingShingle | Jose Manuel Silvan Esperanza Guerrero-Hurtado Alba Gutiérrez-Docio Teresa Alarcón-Cavero Marin Prodanov Adolfo J. Martinez-Rodriguez Olive-Leaf Extracts Modulate Inflammation and Oxidative Stress Associated with Human <i>H. pylori</i> Infection Antioxidants olive-leaf extract <i>Helicobacter pylori</i> antioxidant activity anti-inflammatory activity HPLC-PAD-MS characterization hydroxytyrosol |
| title | Olive-Leaf Extracts Modulate Inflammation and Oxidative Stress Associated with Human <i>H. pylori</i> Infection |
| title_full | Olive-Leaf Extracts Modulate Inflammation and Oxidative Stress Associated with Human <i>H. pylori</i> Infection |
| title_fullStr | Olive-Leaf Extracts Modulate Inflammation and Oxidative Stress Associated with Human <i>H. pylori</i> Infection |
| title_full_unstemmed | Olive-Leaf Extracts Modulate Inflammation and Oxidative Stress Associated with Human <i>H. pylori</i> Infection |
| title_short | Olive-Leaf Extracts Modulate Inflammation and Oxidative Stress Associated with Human <i>H. pylori</i> Infection |
| title_sort | olive leaf extracts modulate inflammation and oxidative stress associated with human i h pylori i infection |
| topic | olive-leaf extract <i>Helicobacter pylori</i> antioxidant activity anti-inflammatory activity HPLC-PAD-MS characterization hydroxytyrosol |
| url | https://www.mdpi.com/2076-3921/10/12/2030 |
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