Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India.
Background: Anopheles culicifacies is an important vector of malaria in Southeast Asia, contributing to almost 70%of malaria cases in India. It exists as a complex of five morphologically indistinguishable species A, B, C, D and E with varied geographical distribution patterns. In India, 8% of the t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2014-12-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/214 |
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| author | Ajay Kumar Sharma Varun Tyagi Sompal Singh Vijay Veer Om Prakash Agrawal Devanathan Sukumaran |
| author_facet | Ajay Kumar Sharma Varun Tyagi Sompal Singh Vijay Veer Om Prakash Agrawal Devanathan Sukumaran |
| author_sort | Ajay Kumar Sharma |
| collection | DOAJ |
| description | Background: Anopheles culicifacies is an important vector of malaria in Southeast Asia, contributing to almost 70%of malaria cases in India. It exists as a complex of five morphologically indistinguishable species A, B, C, D and E with varied geographical distribution patterns. In India, 8% of the total population of Madhya Pradesh (Central India) contributes about 30% of total malaria cases, 60% of total falciparum cases and 50% of malaria deaths. An. culicifacies is the major malaria vector in this state. Vector control mainly relies on the proper identification and distribution of vector species exists in a particular area. The present study was carried out to identify the distribution of An. culicifacies sibling species in certain endemic district of Central India, Madhya Pradesh.
Methods: The An. culicifacies mosquitoes collected from the study districts were identified morphologically. The genomic DNA was isolated from the mosquitoes and subjected to Allele specific PCR targeting D3 domain of 28S ribosomal DNA.
Results: The mean prevalence of An. culicifacies during the study period was in the range of 8–120 per man per hour (PMH). From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively.
Conclusion: This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies. |
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| format | Article |
| id | doaj.art-75688cd443c94d1084aca18452c538b1 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-12T04:30:28Z |
| publishDate | 2014-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-75688cd443c94d1084aca18452c538b12022-12-22T00:38:06ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712014-12-0182192Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India.Ajay Kumar Sharma0Varun Tyagi1Sompal Singh2Vijay Veer3Om Prakash Agrawal4Devanathan Sukumaran5Vector Management Division, Defence Research and Development Establishment, Madhya Pradesh, India.Vector Management Division, Defence Research and Development Establishment, Madhya Pradesh, India.National Institute of Malaria Research, New Delhi, India.Defence Research Laboratory, Assam, India.School of Studies in Zoology, Jiwaji University, Madhya Pradesh, India.Vector Management Division, Defence Research and Development Establishment, Madhya Pradesh, India.Background: Anopheles culicifacies is an important vector of malaria in Southeast Asia, contributing to almost 70%of malaria cases in India. It exists as a complex of five morphologically indistinguishable species A, B, C, D and E with varied geographical distribution patterns. In India, 8% of the total population of Madhya Pradesh (Central India) contributes about 30% of total malaria cases, 60% of total falciparum cases and 50% of malaria deaths. An. culicifacies is the major malaria vector in this state. Vector control mainly relies on the proper identification and distribution of vector species exists in a particular area. The present study was carried out to identify the distribution of An. culicifacies sibling species in certain endemic district of Central India, Madhya Pradesh. Methods: The An. culicifacies mosquitoes collected from the study districts were identified morphologically. The genomic DNA was isolated from the mosquitoes and subjected to Allele specific PCR targeting D3 domain of 28S ribosomal DNA. Results: The mean prevalence of An. culicifacies during the study period was in the range of 8–120 per man per hour (PMH). From the study areas species B was identified from Jabalpur, Chindwara and Hoshangabad, Species C from Hoshangabad only, Species D from Narsinghpur and Khandwa and sibling species E from Mandla, Chindwara and Hoshangabad respectively. Conclusion: This is the first report to detect species E from Madhya Pradesh region which necessitate for reconsideration of species distribution of each An. culicifacies sibling species that would enable to develop required vector control strategies.https://jad.tums.ac.ir/index.php/jad/article/view/214AS-PCRAnopheles culicifaciesMadhya PradeshMalariaSibling species |
| spellingShingle | Ajay Kumar Sharma Varun Tyagi Sompal Singh Vijay Veer Om Prakash Agrawal Devanathan Sukumaran Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India. Journal of Arthropod-Borne Diseases AS-PCR Anopheles culicifacies Madhya Pradesh Malaria Sibling species |
| title | Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India. |
| title_full | Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India. |
| title_fullStr | Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India. |
| title_full_unstemmed | Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India. |
| title_short | Distribution of Anopheles culicifacies and Detection of its Sibling Species E from Madhya Pradesh: Central India. |
| title_sort | distribution of anopheles culicifacies and detection of its sibling species e from madhya pradesh central india |
| topic | AS-PCR Anopheles culicifacies Madhya Pradesh Malaria Sibling species |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/214 |
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