Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection
Abstract Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii, which occurs worldwide in mammals and birds. Brain is the primary target organ because Toxoplasma gondii is a ubiquitous intracellular parasite that causes most frequently life-threatening encephalitis in immunocompromise...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | Laboratory Animal Research |
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| Online Access: | http://link.springer.com/article/10.1186/s42826-019-0007-z |
| _version_ | 1818544363744002048 |
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| author | Hasan Tarik Atmaca Aycan Nuriye Gazyagci Osman Safa Terzi Gungor Cagdas Dincel Tugce Sumer |
| author_facet | Hasan Tarik Atmaca Aycan Nuriye Gazyagci Osman Safa Terzi Gungor Cagdas Dincel Tugce Sumer |
| author_sort | Hasan Tarik Atmaca |
| collection | DOAJ |
| description | Abstract Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii, which occurs worldwide in mammals and birds. Brain is the primary target organ because Toxoplasma gondii is a ubiquitous intracellular parasite that causes most frequently life-threatening encephalitis in immunocompromised patients. Relation of tissue cysts number, histopathology score and acute phase proteins were investigated. In this study, 36 mice are infected with Me49 strain of Toxoplasma gondii. The control group has 6 healthy mice. After inoculation of Toxoplasma gondii, at 10., 15., 20., 30., 45., 60. days, 6 each mice euthanized after collection of blood samples. Hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin levels are determined by ELISA. Then, brain tissues were investigated histopathologically and lesions were scored. The average cyst numbers were determined by counting three samples (25 μl each) of each brain homogenate under light microscopy. Inflammatory reaction was observed on day 10 days after inoculation (d.a.i.) The lesions were characterized by perivascular mononuclear cell infiltration, focal mononuclear cell infiltration in the meninges, and glial proliferation. Tissue cysts were observed in all Toxoplasma gondii-infected groups. The highest lesion score was observed at 60 d.a.i. And the most tissue cyst number were on day 30. d.a.i. Serum levels of hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin were significantly higher than the control group on day 10–20., 10., 10–30., 10.,10–45 d.a.i., respectively. High level of acute phase proteins in mice on certain days infected with Toxoplasma gondii was exhibited a relationship between brain lesions and tissue cysts. |
| first_indexed | 2024-12-11T22:47:31Z |
| format | Article |
| id | doaj.art-7568ff6650c5484fbf84ab2ae6237cbb |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-11T22:47:31Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-7568ff6650c5484fbf84ab2ae6237cbb2022-12-22T00:47:34ZengBMCLaboratory Animal Research2233-76602019-07-013511910.1186/s42826-019-0007-zTracking acute phase protein response during acute and chronic Toxoplasma gondii infectionHasan Tarik Atmaca0Aycan Nuriye Gazyagci1Osman Safa Terzi2Gungor Cagdas Dincel3Tugce Sumer4Department of Pathology, Balikesir University, Faculty of Veterinary MedicineDepartment of Parasitology, Kirikkale University, Faculty of Veterinary MedicineDepartment of Internal Medicine, Ankara University, Faculty of Veterinary MedicineEskil Vocational High School, University of AksarayDepartment of Pathology, Kirikkale University, Faculty of Veterinary MedicineAbstract Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii, which occurs worldwide in mammals and birds. Brain is the primary target organ because Toxoplasma gondii is a ubiquitous intracellular parasite that causes most frequently life-threatening encephalitis in immunocompromised patients. Relation of tissue cysts number, histopathology score and acute phase proteins were investigated. In this study, 36 mice are infected with Me49 strain of Toxoplasma gondii. The control group has 6 healthy mice. After inoculation of Toxoplasma gondii, at 10., 15., 20., 30., 45., 60. days, 6 each mice euthanized after collection of blood samples. Hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin levels are determined by ELISA. Then, brain tissues were investigated histopathologically and lesions were scored. The average cyst numbers were determined by counting three samples (25 μl each) of each brain homogenate under light microscopy. Inflammatory reaction was observed on day 10 days after inoculation (d.a.i.) The lesions were characterized by perivascular mononuclear cell infiltration, focal mononuclear cell infiltration in the meninges, and glial proliferation. Tissue cysts were observed in all Toxoplasma gondii-infected groups. The highest lesion score was observed at 60 d.a.i. And the most tissue cyst number were on day 30. d.a.i. Serum levels of hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin were significantly higher than the control group on day 10–20., 10., 10–30., 10.,10–45 d.a.i., respectively. High level of acute phase proteins in mice on certain days infected with Toxoplasma gondii was exhibited a relationship between brain lesions and tissue cysts.http://link.springer.com/article/10.1186/s42826-019-0007-zToxoplasma gondiiEncephalitisAcute phase proteinsAcuteChronic |
| spellingShingle | Hasan Tarik Atmaca Aycan Nuriye Gazyagci Osman Safa Terzi Gungor Cagdas Dincel Tugce Sumer Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection Laboratory Animal Research Toxoplasma gondii Encephalitis Acute phase proteins Acute Chronic |
| title | Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection |
| title_full | Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection |
| title_fullStr | Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection |
| title_full_unstemmed | Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection |
| title_short | Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection |
| title_sort | tracking acute phase protein response during acute and chronic toxoplasma gondii infection |
| topic | Toxoplasma gondii Encephalitis Acute phase proteins Acute Chronic |
| url | http://link.springer.com/article/10.1186/s42826-019-0007-z |
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