Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironment
Abstract Cancer-associated fibroblasts (CAFs) play a significant role in tumor progression within the tumor microenvironment. Previously, we used near-infrared photoimmunotherapy (NIR-PIT), a next-generation cancer cell-targeted phototherapy, to establish CAF-targeted NIR-PIT. In this study, we inve...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24313-3 |
| _version_ | 1811211181432504320 |
|---|---|
| author | Hiroaki Sato Kazuhiro Noma Toshiaki Ohara Kento Kawasaki Masaaki Akai Teruki Kobayashi Noriyuki Nishiwaki Toru Narusaka Satoshi Komoto Hajime Kashima Yuki Katsura Takuya Kato Satoru Kikuchi Hiroshi Tazawa Shunsuke Kagawa Yasuhiro Shirakawa Hisataka Kobayashi Toshiyoshi Fujiwara |
| author_facet | Hiroaki Sato Kazuhiro Noma Toshiaki Ohara Kento Kawasaki Masaaki Akai Teruki Kobayashi Noriyuki Nishiwaki Toru Narusaka Satoshi Komoto Hajime Kashima Yuki Katsura Takuya Kato Satoru Kikuchi Hiroshi Tazawa Shunsuke Kagawa Yasuhiro Shirakawa Hisataka Kobayashi Toshiyoshi Fujiwara |
| author_sort | Hiroaki Sato |
| collection | DOAJ |
| description | Abstract Cancer-associated fibroblasts (CAFs) play a significant role in tumor progression within the tumor microenvironment. Previously, we used near-infrared photoimmunotherapy (NIR-PIT), a next-generation cancer cell-targeted phototherapy, to establish CAF-targeted NIR-PIT. In this study, we investigated whether dual-targeted NIR-PIT, targeting cancer cells and CAFs, could be a therapeutic strategy. A total of 132 cases of esophageal cancer were analyzed for epidermal growth factor receptor (EGFR), human epidermal growth factor 2 (HER2), and fibroblast activation protein (FAP) expression using immunohistochemistry. Human esophageal cancer cells and CAFs were co-cultured and treated with single- or dual-targeted NIR-PIT in vitro. These cells were co-inoculated into BALB/c-nu/nu mice and the tumors were treated with single-targeted NIR-PIT or dual-targeted NIR-PIT in vivo. Survival analysis showed FAP- or EGFR-high patients had worse survival than patients with low expression of FAP or EGFR (log-rank, P < 0.001 and P = 0.074, respectively), while no difference was observed in HER2 status. In vitro, dual (EGFR/FAP)-targeted NIR-PIT induced specific therapeutic effects in cancer cells and CAFs along with suppressing tumor growth in vivo, whereas single-targeted NIR-PIT did not show any significance. Moreover, these experiments demonstrated that dual-targeted NIR-PIT could treat cancer cells and CAFs simultaneously with a single NIR light irradiation. We demonstrated the relationship between EGFR/FAP expression and prognosis of patients with esophageal cancer and the stronger therapeutic effect of dual-targeted NIR-PIT than single-targeted NIR-PIT in experimental models. Thus, dual-targeted NIR-PIT might be a promising therapeutic strategy for cancer treatment. |
| first_indexed | 2024-04-12T05:08:28Z |
| format | Article |
| id | doaj.art-756adf4384794ffba953a85b09b706b8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T05:08:28Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-756adf4384794ffba953a85b09b706b82022-12-22T03:46:50ZengNature PortfolioScientific Reports2045-23222022-11-0112111410.1038/s41598-022-24313-3Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironmentHiroaki Sato0Kazuhiro Noma1Toshiaki Ohara2Kento Kawasaki3Masaaki Akai4Teruki Kobayashi5Noriyuki Nishiwaki6Toru Narusaka7Satoshi Komoto8Hajime Kashima9Yuki Katsura10Takuya Kato11Satoru Kikuchi12Hiroshi Tazawa13Shunsuke Kagawa14Yasuhiro Shirakawa15Hisataka Kobayashi16Toshiyoshi Fujiwara17Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesMolecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of HealthDepartment of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesAbstract Cancer-associated fibroblasts (CAFs) play a significant role in tumor progression within the tumor microenvironment. Previously, we used near-infrared photoimmunotherapy (NIR-PIT), a next-generation cancer cell-targeted phototherapy, to establish CAF-targeted NIR-PIT. In this study, we investigated whether dual-targeted NIR-PIT, targeting cancer cells and CAFs, could be a therapeutic strategy. A total of 132 cases of esophageal cancer were analyzed for epidermal growth factor receptor (EGFR), human epidermal growth factor 2 (HER2), and fibroblast activation protein (FAP) expression using immunohistochemistry. Human esophageal cancer cells and CAFs were co-cultured and treated with single- or dual-targeted NIR-PIT in vitro. These cells were co-inoculated into BALB/c-nu/nu mice and the tumors were treated with single-targeted NIR-PIT or dual-targeted NIR-PIT in vivo. Survival analysis showed FAP- or EGFR-high patients had worse survival than patients with low expression of FAP or EGFR (log-rank, P < 0.001 and P = 0.074, respectively), while no difference was observed in HER2 status. In vitro, dual (EGFR/FAP)-targeted NIR-PIT induced specific therapeutic effects in cancer cells and CAFs along with suppressing tumor growth in vivo, whereas single-targeted NIR-PIT did not show any significance. Moreover, these experiments demonstrated that dual-targeted NIR-PIT could treat cancer cells and CAFs simultaneously with a single NIR light irradiation. We demonstrated the relationship between EGFR/FAP expression and prognosis of patients with esophageal cancer and the stronger therapeutic effect of dual-targeted NIR-PIT than single-targeted NIR-PIT in experimental models. Thus, dual-targeted NIR-PIT might be a promising therapeutic strategy for cancer treatment.https://doi.org/10.1038/s41598-022-24313-3 |
| spellingShingle | Hiroaki Sato Kazuhiro Noma Toshiaki Ohara Kento Kawasaki Masaaki Akai Teruki Kobayashi Noriyuki Nishiwaki Toru Narusaka Satoshi Komoto Hajime Kashima Yuki Katsura Takuya Kato Satoru Kikuchi Hiroshi Tazawa Shunsuke Kagawa Yasuhiro Shirakawa Hisataka Kobayashi Toshiyoshi Fujiwara Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironment Scientific Reports |
| title | Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironment |
| title_full | Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironment |
| title_fullStr | Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironment |
| title_full_unstemmed | Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironment |
| title_short | Dual-targeted near-infrared photoimmunotherapy for esophageal cancer and cancer-associated fibroblasts in the tumor microenvironment |
| title_sort | dual targeted near infrared photoimmunotherapy for esophageal cancer and cancer associated fibroblasts in the tumor microenvironment |
| url | https://doi.org/10.1038/s41598-022-24313-3 |
| work_keys_str_mv | AT hiroakisato dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT kazuhironoma dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT toshiakiohara dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT kentokawasaki dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT masaakiakai dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT terukikobayashi dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT noriyukinishiwaki dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT torunarusaka dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT satoshikomoto dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT hajimekashima dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT yukikatsura dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT takuyakato dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT satorukikuchi dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT hiroshitazawa dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT shunsukekagawa dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT yasuhiroshirakawa dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT hisatakakobayashi dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment AT toshiyoshifujiwara dualtargetednearinfraredphotoimmunotherapyforesophagealcancerandcancerassociatedfibroblastsinthetumormicroenvironment |