First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs
Plasmid-mediated fosfomycin determinants is a global public health concern due to the increasing dissemination of fosfomycin resistance and limited clinical treatment options. Information about the fosfomycin resistant and molecular genetic among <i>Enterococcus</i> spp. is still lacking...
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MDPI AG
2021-10-01
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| Series: | Genes |
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| author | Xiaoming Wang Yi Gao Xiao Liu Naiyan Sun Jinhu Huang Liping Wang |
| author_facet | Xiaoming Wang Yi Gao Xiao Liu Naiyan Sun Jinhu Huang Liping Wang |
| author_sort | Xiaoming Wang |
| collection | DOAJ |
| description | Plasmid-mediated fosfomycin determinants is a global public health concern due to the increasing dissemination of fosfomycin resistance and limited clinical treatment options. Information about the fosfomycin resistant and molecular genetic among <i>Enterococcus</i> spp. is still lacking. In this study, we found the first plasmid-medieted <i>fosB</i> in <i>Enterococcus faecalis</i> from pigs, and all the fosfomycin resistant <i>Enterococcus</i> spp. (FRE) isolates were multi-drug resistant. S1-PFGE, Southern blot and conjugation experiments indicated that the <i>fosB</i> gene located on ~54.7 kb transferable plasmids<sub>.</sub> Relative competition assay confirmed that the <i>fosB</i><i>-</i>carrying plasmid impaired fitness in recipient <i>E. faecalis</i> JH2-2. Illumina and the MinION sequencing data revealed that both <i>E. faecalis</i> ES-1 and ES-2 isolates belonged to novel ST (ST964), and had 71 SNPs difference. WGS showed that the genetic environments of <i>fosB</i> were diverse among different species, and the linezolid resistance gene <i>optrA</i> was found in the <i>fosB</i>-carrying strains. To summarize, for the first time, we reported plasmid-mediated <i>fosB</i> in <i>E. faecalis</i> from pigs. And, the co-occurrence of <i>fosB</i> and <i>optrA</i> pose a serious threat to public health. |
| first_indexed | 2024-03-10T05:28:35Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-10T05:28:35Z |
| publishDate | 2021-10-01 |
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| series | Genes |
| spelling | doaj.art-756b7b2c26da4b8db3751f54b750baab2023-11-22T23:27:16ZengMDPI AGGenes2073-44252021-10-011211168410.3390/genes12111684First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from PigsXiaoming Wang0Yi Gao1Xiao Liu2Naiyan Sun3Jinhu Huang4Liping Wang5MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, ChinaPlasmid-mediated fosfomycin determinants is a global public health concern due to the increasing dissemination of fosfomycin resistance and limited clinical treatment options. Information about the fosfomycin resistant and molecular genetic among <i>Enterococcus</i> spp. is still lacking. In this study, we found the first plasmid-medieted <i>fosB</i> in <i>Enterococcus faecalis</i> from pigs, and all the fosfomycin resistant <i>Enterococcus</i> spp. (FRE) isolates were multi-drug resistant. S1-PFGE, Southern blot and conjugation experiments indicated that the <i>fosB</i> gene located on ~54.7 kb transferable plasmids<sub>.</sub> Relative competition assay confirmed that the <i>fosB</i><i>-</i>carrying plasmid impaired fitness in recipient <i>E. faecalis</i> JH2-2. Illumina and the MinION sequencing data revealed that both <i>E. faecalis</i> ES-1 and ES-2 isolates belonged to novel ST (ST964), and had 71 SNPs difference. WGS showed that the genetic environments of <i>fosB</i> were diverse among different species, and the linezolid resistance gene <i>optrA</i> was found in the <i>fosB</i>-carrying strains. To summarize, for the first time, we reported plasmid-mediated <i>fosB</i> in <i>E. faecalis</i> from pigs. And, the co-occurrence of <i>fosB</i> and <i>optrA</i> pose a serious threat to public health.https://www.mdpi.com/2073-4425/12/11/1684antimicrobial resistancemolecular geneticfosfomycin resistant<i>Enterococcus</i> spp. |
| spellingShingle | Xiaoming Wang Yi Gao Xiao Liu Naiyan Sun Jinhu Huang Liping Wang First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs Genes antimicrobial resistance molecular genetic fosfomycin resistant <i>Enterococcus</i> spp. |
| title | First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_full | First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_fullStr | First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_full_unstemmed | First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_short | First Report of the Plasmid-mediated <i>fosB</i> Gene in <i>Enterococcus faecalis</i> from Pigs |
| title_sort | first report of the plasmid mediated i fosb i gene in i enterococcus faecalis i from pigs |
| topic | antimicrobial resistance molecular genetic fosfomycin resistant <i>Enterococcus</i> spp. |
| url | https://www.mdpi.com/2073-4425/12/11/1684 |
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