Circulating microRNAs as Biomarkers of Hepatic Fibrosis in Schistosomiasis Japonica Patients in the Philippines
Host-derived microRNAs (miRNAs) play important regulatory roles in schistosomiasis-induced hepatic fibrosis. This study analyzed selected serum miRNAs among Filipino schistosomiasis japonica patients with ultrasound (US)-detectable hepatic fibrosis. A prospective cohort study design with convenience...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/8/1902 |
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| author | Ian Kim B. Tabios Marcello Otake Sato Ourlad Alzeus Gaddi Tantengco Raffy Jay C. Fornillos Masashi Kirinoki Megumi Sato Raniv D. Rojo Ian Kendrich C. Fontanilla Yuichi Chigusa Paul Mark B. Medina Mihoko Kikuchi Lydia R. Leonardo |
| author_facet | Ian Kim B. Tabios Marcello Otake Sato Ourlad Alzeus Gaddi Tantengco Raffy Jay C. Fornillos Masashi Kirinoki Megumi Sato Raniv D. Rojo Ian Kendrich C. Fontanilla Yuichi Chigusa Paul Mark B. Medina Mihoko Kikuchi Lydia R. Leonardo |
| author_sort | Ian Kim B. Tabios |
| collection | DOAJ |
| description | Host-derived microRNAs (miRNAs) play important regulatory roles in schistosomiasis-induced hepatic fibrosis. This study analyzed selected serum miRNAs among Filipino schistosomiasis japonica patients with ultrasound (US)-detectable hepatic fibrosis. A prospective cohort study design with convenience sampling was employed from 2017 to 2019. The study sites were eight endemic barangays in Leyte, Philippines. Eligible chronic schistosomiasis patients with varying severities of hepatic fibrosis were enrolled in the cohort and serially examined at 6, 12, and 24 months from baseline. Baseline serum miR-146a-5p, let-7a-5p, miR-150-5p, miR-122-5p, miR-93-5p, and miR200b-3p were measured using RT-qPCR. A total of 136 chronic schistosomiasis patients were included in this prospective cohort study. Approximately, 42.6% had no fibrosis, 22.8% had mild fibrosis, and 34.6% had severe fibrosis at baseline The serum levels of the antifibrotic miR-146a (<i>p</i> < 0.0001), miR-150 (<i>p</i> = 0.0058), and let-7a (<i>p</i> < 0.0001) were significantly lower in patients with hepatic fibrosis while the profibrotic miR-93 (<i>p</i> = 0.0024) was elevated. miR-146a-5p (AUC = 0.90, 95% CI [0.84, 0.96], <i>p</i> < 0.0001) has the most promising potential to differentiate patients with (<i>n</i> = 78) versus without (<i>n</i> = 58) hepatic fibrosis. The baseline level of serum miR-146-5p was significantly different in patients with progressive fibrosis (<i>n</i> = 17) compared to those who never developed fibrosis (<i>n</i> = 30, <i>p</i> < 0.01) or those who had fibrosis reversal (<i>n</i> = 20, <i>p</i> < 0.01) after 24 months. These findings demonstrate the potential utility of serum miRNAs, particularly of miR-146a, as a supplementary tool for assessing hepatic fibrosis in chronic schistosomiasis japonica patients. |
| first_indexed | 2024-03-09T13:35:06Z |
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| id | doaj.art-756c3652f95645c09d25e0fae8e746a3 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T13:35:06Z |
| publishDate | 2022-08-01 |
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| series | Diagnostics |
| spelling | doaj.art-756c3652f95645c09d25e0fae8e746a32023-11-30T21:13:17ZengMDPI AGDiagnostics2075-44182022-08-01128190210.3390/diagnostics12081902Circulating microRNAs as Biomarkers of Hepatic Fibrosis in Schistosomiasis Japonica Patients in the PhilippinesIan Kim B. Tabios0Marcello Otake Sato1Ourlad Alzeus Gaddi Tantengco2Raffy Jay C. Fornillos3Masashi Kirinoki4Megumi Sato5Raniv D. Rojo6Ian Kendrich C. Fontanilla7Yuichi Chigusa8Paul Mark B. Medina9Mihoko Kikuchi10Lydia R. Leonardo11Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesLaboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, 880 Kitakobayashi, Mibu-machi, Shimotsuga-gun, Tochigi 321-0293, JapanCollege of Medicine, University of the Philippines Manila, Ermita, Manilla 1000, PhilippinesInstitute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesLaboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, 880 Kitakobayashi, Mibu-machi, Shimotsuga-gun, Tochigi 321-0293, JapanGraduate School of Health Sciences, Niigata University, 2-746 Asahimachi-dori, Chuo-ku, Niigata City 951-8518, JapanCollege of Medicine, University of the Philippines Manila, Ermita, Manilla 1000, PhilippinesInstitute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesCenter for International Cooperation, Dokkyo Medical University, 880 Kitakobayashi, Mibu-machi, Shimotsuga-gun, Tochigi 321-0293, JapanDepartment of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Ermita, Manilla 1000, PhilippinesInstitute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, JapanInstitute of Biology, College of Science, University of the Philippines Diliman, Quezon City 1101, PhilippinesHost-derived microRNAs (miRNAs) play important regulatory roles in schistosomiasis-induced hepatic fibrosis. This study analyzed selected serum miRNAs among Filipino schistosomiasis japonica patients with ultrasound (US)-detectable hepatic fibrosis. A prospective cohort study design with convenience sampling was employed from 2017 to 2019. The study sites were eight endemic barangays in Leyte, Philippines. Eligible chronic schistosomiasis patients with varying severities of hepatic fibrosis were enrolled in the cohort and serially examined at 6, 12, and 24 months from baseline. Baseline serum miR-146a-5p, let-7a-5p, miR-150-5p, miR-122-5p, miR-93-5p, and miR200b-3p were measured using RT-qPCR. A total of 136 chronic schistosomiasis patients were included in this prospective cohort study. Approximately, 42.6% had no fibrosis, 22.8% had mild fibrosis, and 34.6% had severe fibrosis at baseline The serum levels of the antifibrotic miR-146a (<i>p</i> < 0.0001), miR-150 (<i>p</i> = 0.0058), and let-7a (<i>p</i> < 0.0001) were significantly lower in patients with hepatic fibrosis while the profibrotic miR-93 (<i>p</i> = 0.0024) was elevated. miR-146a-5p (AUC = 0.90, 95% CI [0.84, 0.96], <i>p</i> < 0.0001) has the most promising potential to differentiate patients with (<i>n</i> = 78) versus without (<i>n</i> = 58) hepatic fibrosis. The baseline level of serum miR-146-5p was significantly different in patients with progressive fibrosis (<i>n</i> = 17) compared to those who never developed fibrosis (<i>n</i> = 30, <i>p</i> < 0.01) or those who had fibrosis reversal (<i>n</i> = 20, <i>p</i> < 0.01) after 24 months. These findings demonstrate the potential utility of serum miRNAs, particularly of miR-146a, as a supplementary tool for assessing hepatic fibrosis in chronic schistosomiasis japonica patients.https://www.mdpi.com/2075-4418/12/8/1902biomarkermiRNAprognosisschistosome<i>Schistosoma japonica</i> |
| spellingShingle | Ian Kim B. Tabios Marcello Otake Sato Ourlad Alzeus Gaddi Tantengco Raffy Jay C. Fornillos Masashi Kirinoki Megumi Sato Raniv D. Rojo Ian Kendrich C. Fontanilla Yuichi Chigusa Paul Mark B. Medina Mihoko Kikuchi Lydia R. Leonardo Circulating microRNAs as Biomarkers of Hepatic Fibrosis in Schistosomiasis Japonica Patients in the Philippines Diagnostics biomarker miRNA prognosis schistosome <i>Schistosoma japonica</i> |
| title | Circulating microRNAs as Biomarkers of Hepatic Fibrosis in Schistosomiasis Japonica Patients in the Philippines |
| title_full | Circulating microRNAs as Biomarkers of Hepatic Fibrosis in Schistosomiasis Japonica Patients in the Philippines |
| title_fullStr | Circulating microRNAs as Biomarkers of Hepatic Fibrosis in Schistosomiasis Japonica Patients in the Philippines |
| title_full_unstemmed | Circulating microRNAs as Biomarkers of Hepatic Fibrosis in Schistosomiasis Japonica Patients in the Philippines |
| title_short | Circulating microRNAs as Biomarkers of Hepatic Fibrosis in Schistosomiasis Japonica Patients in the Philippines |
| title_sort | circulating micrornas as biomarkers of hepatic fibrosis in schistosomiasis japonica patients in the philippines |
| topic | biomarker miRNA prognosis schistosome <i>Schistosoma japonica</i> |
| url | https://www.mdpi.com/2075-4418/12/8/1902 |
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