Nicotine Facilitates Facial Stimulation-Evoked Mossy Fiber-Granule Cell Long-Term Potentiation in vivo in Mice
Nicotine is a psychoactive component of tobacco that plays critical roles in the regulation of neuronal circuit function and neuroplasticity and contributes to the improvement of working memory performance and motor learning function via nicotinic acetylcholine receptors (nAChRs). Under in vivo cond...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
|
| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2022.905724/full |
| _version_ | 1828469731061923840 |
|---|---|
| author | Li-Xin Cao Yan-Hua Bing Yin-Hua Xu Guang-Jian Zhang Chun-Ping Chu Lan Hong De-Lai Qiu |
| author_facet | Li-Xin Cao Yan-Hua Bing Yin-Hua Xu Guang-Jian Zhang Chun-Ping Chu Lan Hong De-Lai Qiu |
| author_sort | Li-Xin Cao |
| collection | DOAJ |
| description | Nicotine is a psychoactive component of tobacco that plays critical roles in the regulation of neuronal circuit function and neuroplasticity and contributes to the improvement of working memory performance and motor learning function via nicotinic acetylcholine receptors (nAChRs). Under in vivo conditions, nicotine enhances facial stimulation-evoked mossy fiber-granule cell (MF-GrC) synaptic transmission, which suggests that nicotine regulates MF-GrC synaptic plasticity in the mouse cerebellar cortex. In this study, we investigated the effects of nicotine on facial stimulation-induced long-term potentiation (LTP) of MF-GrC synaptic transmission in urethane-anesthetized mice. Our results showed that facial stimulation at 20 Hz induced an MF-GrC LTP in the mouse cerebellar granular layer that was significantly enhanced by the application of nicotine (1 μM). Blockade of α4β2 nAChRs, but not α7 nAChRs, during delivery of 20 Hz facial stimulation prevented the nicotine-induced facilitation of MF-GrC LTP. Notably, the facial stimulation-induced MF-GrC LTP was abolished by an N-methyl-D-aspartate (NMDA) receptor antagonist, but it was restored by additional application of nicotine during delivery of 20 Hz facial stimulation. Furthermore, antagonism of α4β2 nAChRs, but not α7 nAChRs, during delivery of 20 Hz facial stimulation prevented nicotine-induced MF-GrC LTP. Moreover, inhibition of nitric oxide synthase (NOS) abolished the facial stimulation-induced MF-GrC LTP, as well as the effect of nicotine on it. Our results indicated that 20 Hz facial stimulation induced MF-GrC LTP via an NMDA receptor/nitric oxide (NO) cascade, but MF-GrC LTP was enhanced by nicotine through the α4β2 AChR/NO signaling pathway. These results suggest that nicotine-induced facilitation of MF-GrC LTP may play a critical role in the improvement of working memory performance and motor learning function. |
| first_indexed | 2024-12-11T04:44:38Z |
| format | Article |
| id | doaj.art-756e0e3f5fcb44debfcf358f13e8ffe2 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-11T04:44:38Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-756e0e3f5fcb44debfcf358f13e8ffe22022-12-22T01:20:33ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022022-07-011610.3389/fncel.2022.905724905724Nicotine Facilitates Facial Stimulation-Evoked Mossy Fiber-Granule Cell Long-Term Potentiation in vivo in MiceLi-Xin Cao0Yan-Hua Bing1Yin-Hua Xu2Guang-Jian Zhang3Chun-Ping Chu4Lan Hong5De-Lai Qiu6Department of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, ChinaDepartment of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, ChinaDepartment of Neurology, Affiliated Hospital of Yanbian University, Yanji, ChinaDepartment of Pain, Affiliated Hospital of Yanbian University, Yanji, ChinaDepartment of Physiology, College of Basic Medicine, Jilin Medical University, Jilin City, ChinaDepartment of Physiology and Pathophysiology, College of Medicine, Yanbian University, Yanji, ChinaDepartment of Physiology, College of Basic Medicine, Jilin Medical University, Jilin City, ChinaNicotine is a psychoactive component of tobacco that plays critical roles in the regulation of neuronal circuit function and neuroplasticity and contributes to the improvement of working memory performance and motor learning function via nicotinic acetylcholine receptors (nAChRs). Under in vivo conditions, nicotine enhances facial stimulation-evoked mossy fiber-granule cell (MF-GrC) synaptic transmission, which suggests that nicotine regulates MF-GrC synaptic plasticity in the mouse cerebellar cortex. In this study, we investigated the effects of nicotine on facial stimulation-induced long-term potentiation (LTP) of MF-GrC synaptic transmission in urethane-anesthetized mice. Our results showed that facial stimulation at 20 Hz induced an MF-GrC LTP in the mouse cerebellar granular layer that was significantly enhanced by the application of nicotine (1 μM). Blockade of α4β2 nAChRs, but not α7 nAChRs, during delivery of 20 Hz facial stimulation prevented the nicotine-induced facilitation of MF-GrC LTP. Notably, the facial stimulation-induced MF-GrC LTP was abolished by an N-methyl-D-aspartate (NMDA) receptor antagonist, but it was restored by additional application of nicotine during delivery of 20 Hz facial stimulation. Furthermore, antagonism of α4β2 nAChRs, but not α7 nAChRs, during delivery of 20 Hz facial stimulation prevented nicotine-induced MF-GrC LTP. Moreover, inhibition of nitric oxide synthase (NOS) abolished the facial stimulation-induced MF-GrC LTP, as well as the effect of nicotine on it. Our results indicated that 20 Hz facial stimulation induced MF-GrC LTP via an NMDA receptor/nitric oxide (NO) cascade, but MF-GrC LTP was enhanced by nicotine through the α4β2 AChR/NO signaling pathway. These results suggest that nicotine-induced facilitation of MF-GrC LTP may play a critical role in the improvement of working memory performance and motor learning function.https://www.frontiersin.org/articles/10.3389/fncel.2022.905724/fullcerebellarfacial stimulationmossy fiber-granular celllong-term potentiation (LTP)nicotineneuropharmacology |
| spellingShingle | Li-Xin Cao Yan-Hua Bing Yin-Hua Xu Guang-Jian Zhang Chun-Ping Chu Lan Hong De-Lai Qiu Nicotine Facilitates Facial Stimulation-Evoked Mossy Fiber-Granule Cell Long-Term Potentiation in vivo in Mice Frontiers in Cellular Neuroscience cerebellar facial stimulation mossy fiber-granular cell long-term potentiation (LTP) nicotine neuropharmacology |
| title | Nicotine Facilitates Facial Stimulation-Evoked Mossy Fiber-Granule Cell Long-Term Potentiation in vivo in Mice |
| title_full | Nicotine Facilitates Facial Stimulation-Evoked Mossy Fiber-Granule Cell Long-Term Potentiation in vivo in Mice |
| title_fullStr | Nicotine Facilitates Facial Stimulation-Evoked Mossy Fiber-Granule Cell Long-Term Potentiation in vivo in Mice |
| title_full_unstemmed | Nicotine Facilitates Facial Stimulation-Evoked Mossy Fiber-Granule Cell Long-Term Potentiation in vivo in Mice |
| title_short | Nicotine Facilitates Facial Stimulation-Evoked Mossy Fiber-Granule Cell Long-Term Potentiation in vivo in Mice |
| title_sort | nicotine facilitates facial stimulation evoked mossy fiber granule cell long term potentiation in vivo in mice |
| topic | cerebellar facial stimulation mossy fiber-granular cell long-term potentiation (LTP) nicotine neuropharmacology |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2022.905724/full |
| work_keys_str_mv | AT lixincao nicotinefacilitatesfacialstimulationevokedmossyfibergranulecelllongtermpotentiationinvivoinmice AT yanhuabing nicotinefacilitatesfacialstimulationevokedmossyfibergranulecelllongtermpotentiationinvivoinmice AT yinhuaxu nicotinefacilitatesfacialstimulationevokedmossyfibergranulecelllongtermpotentiationinvivoinmice AT guangjianzhang nicotinefacilitatesfacialstimulationevokedmossyfibergranulecelllongtermpotentiationinvivoinmice AT chunpingchu nicotinefacilitatesfacialstimulationevokedmossyfibergranulecelllongtermpotentiationinvivoinmice AT lanhong nicotinefacilitatesfacialstimulationevokedmossyfibergranulecelllongtermpotentiationinvivoinmice AT delaiqiu nicotinefacilitatesfacialstimulationevokedmossyfibergranulecelllongtermpotentiationinvivoinmice |