Assessment of repeat dose toxicity of Cochleate derived from Neisseria meningitidis proteoliposome in Sprague Dawley rats
The AFCo1 cochleate is a potential novel adjuvant derived from Neisseria meningitidis B proteoliposome. The aim of this study was to assess the safety of AFCo1 by repeated doses in Sprague Dawley rats. Rats were grouped for treatment with AFCo1, placebo formulation or control. Four similar doses of...
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| Format: | Article |
| Language: | English |
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Finlay Ediciones
2012-12-01
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| Series: | VacciMonitor |
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| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2012000300001&lng=es&nrm=iso&tlng=en |
| _version_ | 1818390997541847040 |
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| author | Juan Francisco Infante Sergio Sifontes Daniel Francisco Arencibia Tamara Hernández Mildrey Fariñas Oliver Pérez |
| author_facet | Juan Francisco Infante Sergio Sifontes Daniel Francisco Arencibia Tamara Hernández Mildrey Fariñas Oliver Pérez |
| author_sort | Juan Francisco Infante |
| collection | DOAJ |
| description | The AFCo1 cochleate is a potential novel adjuvant derived from Neisseria meningitidis B proteoliposome. The aim of this study was to assess the safety of AFCo1 by repeated doses in Sprague Dawley rats. Rats were grouped for treatment with AFCo1, placebo formulation or control. Four similar doses of the test substance were instilled every five days. Intranasal dose of 100 µL was used, and the body weight, water and food intakes were monitored as well as the clinical symptoms. Rats were sacrificed at 3, 14 and 28 days after the last inoculation and anatomopathological studies were conducted. Clinical observations were carried out for the study and a number of rats from each group were sacrificed 3 and 14 days after the last dose in order to conduct hematological, hemochemical and anatomopathological studies. Clinical symptoms, food and water intakes, and body weight did not show differences of toxicological relevance. The histological changes found were mild and similar in the three groups. AFCo1 is potentially safe by nasal route for human use as evidenced by the absence of local and systemic signs of toxicity in Sprague Dawley rats. |
| first_indexed | 2024-12-14T05:06:31Z |
| format | Article |
| id | doaj.art-756e2938215b47558b3c32558cdb3fd7 |
| institution | Directory Open Access Journal |
| issn | 1025-028X 1025-0298 |
| language | English |
| last_indexed | 2024-12-14T05:06:31Z |
| publishDate | 2012-12-01 |
| publisher | Finlay Ediciones |
| record_format | Article |
| series | VacciMonitor |
| spelling | doaj.art-756e2938215b47558b3c32558cdb3fd72022-12-21T23:16:06ZengFinlay EdicionesVacciMonitor1025-028X1025-02982012-12-0121315Assessment of repeat dose toxicity of Cochleate derived from Neisseria meningitidis proteoliposome in Sprague Dawley ratsJuan Francisco Infante 0Sergio Sifontes1Daniel Francisco Arencibia 2Tamara Hernández3Mildrey Fariñas4Oliver Pérez 5Research and Development Vicepresidency of Finlay Institute, Havana, Cuba.Research and Development Vicepresidency of Finlay Institute, Havana, Cuba.Research and Development Vicepresidency of Finlay Institute, Havana, Cuba.Research and Development Vicepresidency of Finlay Institute, Havana, Cuba.Research and Development Vicepresidency of Finlay Institute, Havana, Cuba.Research and Development Vicepresidency of Finlay Institute, Havana, Cuba.The AFCo1 cochleate is a potential novel adjuvant derived from Neisseria meningitidis B proteoliposome. The aim of this study was to assess the safety of AFCo1 by repeated doses in Sprague Dawley rats. Rats were grouped for treatment with AFCo1, placebo formulation or control. Four similar doses of the test substance were instilled every five days. Intranasal dose of 100 µL was used, and the body weight, water and food intakes were monitored as well as the clinical symptoms. Rats were sacrificed at 3, 14 and 28 days after the last inoculation and anatomopathological studies were conducted. Clinical observations were carried out for the study and a number of rats from each group were sacrificed 3 and 14 days after the last dose in order to conduct hematological, hemochemical and anatomopathological studies. Clinical symptoms, food and water intakes, and body weight did not show differences of toxicological relevance. The histological changes found were mild and similar in the three groups. AFCo1 is potentially safe by nasal route for human use as evidenced by the absence of local and systemic signs of toxicity in Sprague Dawley rats.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2012000300001&lng=es&nrm=iso&tlng=enneisseria meningitidisSprague Dawleyrepeated doseproteoliposome |
| spellingShingle | Juan Francisco Infante Sergio Sifontes Daniel Francisco Arencibia Tamara Hernández Mildrey Fariñas Oliver Pérez Assessment of repeat dose toxicity of Cochleate derived from Neisseria meningitidis proteoliposome in Sprague Dawley rats VacciMonitor neisseria meningitidis Sprague Dawley repeated dose proteoliposome |
| title | Assessment of repeat dose toxicity of Cochleate derived from Neisseria meningitidis proteoliposome in Sprague Dawley rats |
| title_full | Assessment of repeat dose toxicity of Cochleate derived from Neisseria meningitidis proteoliposome in Sprague Dawley rats |
| title_fullStr | Assessment of repeat dose toxicity of Cochleate derived from Neisseria meningitidis proteoliposome in Sprague Dawley rats |
| title_full_unstemmed | Assessment of repeat dose toxicity of Cochleate derived from Neisseria meningitidis proteoliposome in Sprague Dawley rats |
| title_short | Assessment of repeat dose toxicity of Cochleate derived from Neisseria meningitidis proteoliposome in Sprague Dawley rats |
| title_sort | assessment of repeat dose toxicity of cochleate derived from neisseria meningitidis proteoliposome in sprague dawley rats |
| topic | neisseria meningitidis Sprague Dawley repeated dose proteoliposome |
| url | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2012000300001&lng=es&nrm=iso&tlng=en |
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