Antimicrobial Activity of a 3D-Printed Polymethylmethacrylate Dental Resin Enhanced with Graphene
The present study aimed to test, in vitro, the antimicrobial activity against <i>Candida albicans</i> and <i>Streptococcus mutans</i> and the surface roughness of a 3D-printed polymethylmethacrylate dental resin enhanced with graphene. A 3D-printed polymethylmethacrylate dent...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/10/2607 |
| _version_ | 1797474999531995136 |
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| author | Helena Salgado Ana T. P. C. Gomes Ana S. Duarte José M. F. Ferreira Carlos Fernandes Maria Helena Figueiral Pedro Mesquita |
| author_facet | Helena Salgado Ana T. P. C. Gomes Ana S. Duarte José M. F. Ferreira Carlos Fernandes Maria Helena Figueiral Pedro Mesquita |
| author_sort | Helena Salgado |
| collection | DOAJ |
| description | The present study aimed to test, in vitro, the antimicrobial activity against <i>Candida albicans</i> and <i>Streptococcus mutans</i> and the surface roughness of a 3D-printed polymethylmethacrylate dental resin enhanced with graphene. A 3D-printed polymethylmethacrylate dental resin was reinforced with four different concentrations of graphene: 0.01, 0.1, 0.25 and 0.5 wt%. Neat resin was used as a control. The specimens were printed in a liquid crystal display printer. Disc specimens were used in antimicrobial evaluation, and bar-shaped specimens were used to measure surface roughness. The study of antimicrobial activity included the inhibition of the growth of <i>C. albicans</i> and <i>S. mutans</i> and their adhesion to the resin’s surface. Surface roughness increased with the increase in the graphene concentration. The growth inhibition of <i>C. albicans</i> was observed in the different concentrations of graphene after 24 h, with no recovery after 48 h. The specimens doped with graphene were capable of inactivating <i>S. mutans</i> after 48 h. The surface-adhesion studies showed that the density of microbial biofilms decreases in the case of specimens doped with graphene. Graphene, despite increasing the resin’s surface roughness, was effective in inhibiting the growth and the adhesion to the resin’s surface of the main inducers of prosthetic stomatitis. |
| first_indexed | 2024-03-09T20:37:55Z |
| format | Article |
| id | doaj.art-75730d67c6074f3fa1d988604acc2c10 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T20:37:55Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-75730d67c6074f3fa1d988604acc2c102023-11-23T23:05:51ZengMDPI AGBiomedicines2227-90592022-10-011010260710.3390/biomedicines10102607Antimicrobial Activity of a 3D-Printed Polymethylmethacrylate Dental Resin Enhanced with GrapheneHelena Salgado0Ana T. P. C. Gomes1Ana S. Duarte2José M. F. Ferreira3Carlos Fernandes4Maria Helena Figueiral5Pedro Mesquita6Faculty of Dental Medicine (FMD), Universidade Católica Portuguesa, 3504-505 Viseu, PortugalCentre of Interdisciplinary Research in Health, Faculty of Dental Medicine (FMD), Universidade Católica Portuguesa, 3504-505 Viseu, PortugalCentre of Interdisciplinary Research in Health, Faculty of Dental Medicine (FMD), Universidade Católica Portuguesa, 3504-505 Viseu, PortugalDepartment of Materials and Ceramic Engineering, CICECO, University of Aveiro, 3810-193 Aveiro, PortugalFaculty of Engeneering (FEUP), Universidade do Porto, 4200-465 Porto, PortugalFaculty of Dental Medicine, Universidade do Porto, 4200-393 Porto, PortugalFaculty of Dental Medicine, Universidade do Porto, 4200-393 Porto, PortugalThe present study aimed to test, in vitro, the antimicrobial activity against <i>Candida albicans</i> and <i>Streptococcus mutans</i> and the surface roughness of a 3D-printed polymethylmethacrylate dental resin enhanced with graphene. A 3D-printed polymethylmethacrylate dental resin was reinforced with four different concentrations of graphene: 0.01, 0.1, 0.25 and 0.5 wt%. Neat resin was used as a control. The specimens were printed in a liquid crystal display printer. Disc specimens were used in antimicrobial evaluation, and bar-shaped specimens were used to measure surface roughness. The study of antimicrobial activity included the inhibition of the growth of <i>C. albicans</i> and <i>S. mutans</i> and their adhesion to the resin’s surface. Surface roughness increased with the increase in the graphene concentration. The growth inhibition of <i>C. albicans</i> was observed in the different concentrations of graphene after 24 h, with no recovery after 48 h. The specimens doped with graphene were capable of inactivating <i>S. mutans</i> after 48 h. The surface-adhesion studies showed that the density of microbial biofilms decreases in the case of specimens doped with graphene. Graphene, despite increasing the resin’s surface roughness, was effective in inhibiting the growth and the adhesion to the resin’s surface of the main inducers of prosthetic stomatitis.https://www.mdpi.com/2227-9059/10/10/2607polymethyl methacrylategraphene<i>Candida albicans</i><i>Streptococcus mutans</i>denture stomatitis3D printing |
| spellingShingle | Helena Salgado Ana T. P. C. Gomes Ana S. Duarte José M. F. Ferreira Carlos Fernandes Maria Helena Figueiral Pedro Mesquita Antimicrobial Activity of a 3D-Printed Polymethylmethacrylate Dental Resin Enhanced with Graphene Biomedicines polymethyl methacrylate graphene <i>Candida albicans</i> <i>Streptococcus mutans</i> denture stomatitis 3D printing |
| title | Antimicrobial Activity of a 3D-Printed Polymethylmethacrylate Dental Resin Enhanced with Graphene |
| title_full | Antimicrobial Activity of a 3D-Printed Polymethylmethacrylate Dental Resin Enhanced with Graphene |
| title_fullStr | Antimicrobial Activity of a 3D-Printed Polymethylmethacrylate Dental Resin Enhanced with Graphene |
| title_full_unstemmed | Antimicrobial Activity of a 3D-Printed Polymethylmethacrylate Dental Resin Enhanced with Graphene |
| title_short | Antimicrobial Activity of a 3D-Printed Polymethylmethacrylate Dental Resin Enhanced with Graphene |
| title_sort | antimicrobial activity of a 3d printed polymethylmethacrylate dental resin enhanced with graphene |
| topic | polymethyl methacrylate graphene <i>Candida albicans</i> <i>Streptococcus mutans</i> denture stomatitis 3D printing |
| url | https://www.mdpi.com/2227-9059/10/10/2607 |
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