Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells
Abstract Purpose The green synthesis of nanoparticles has recently opened up a new route in material production. The aim of this study was to evaluate the effect of nanohydroxyapatite (nHA) synthesized from Elaeagnus angustifolia (EA) extract in polycaprolactone (PCL) nanofibers (PCL/nHAEA) to odont...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-022-02527-1 |
| _version_ | 1797984071645659136 |
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| author | Ehsaneh Azaryan Mohammad Yahya Hanafi-Bojd Esmat Alemzadeh Fariba Emadian Razavi Mohsen Naseri |
| author_facet | Ehsaneh Azaryan Mohammad Yahya Hanafi-Bojd Esmat Alemzadeh Fariba Emadian Razavi Mohsen Naseri |
| author_sort | Ehsaneh Azaryan |
| collection | DOAJ |
| description | Abstract Purpose The green synthesis of nanoparticles has recently opened up a new route in material production. The aim of this study was to evaluate the effect of nanohydroxyapatite (nHA) synthesized from Elaeagnus angustifolia (EA) extract in polycaprolactone (PCL) nanofibers (PCL/nHAEA) to odontogenic differentiation of dental pulp stem cells (DPSCs) and their potential applications for dentin tissue engineering. Methods Green synthesis of nHA via EA extract (nHAEA) was done by the sol–gel technique. Then electrospun nanocomposites containing of PCL blended with nHA (P/nHA) and nHAEA (P/nHAEA) were fabricated, and the characterization was evaluated via X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and the contact angle. The morphology of nanofibers and the cell adhesion capacity of DPSCs on nanofibers were evaluated using SEM. Cytocompatibility was assessed by MTT. Osteo/odontogenic differentiation ability of the nanocomposites were assessed using alkaline phosphatase (ALP) activity, alizarin red S (ARS) staining, and quantitative real-time polymerase chain reaction (qPCR) technique. Results Viability and adhesion capacity of DPSCs were higher on P/nHAEA nanofibers than PCL and P/nHA nanofibers. ARS assay, ALP activity, and qPCR analysis findings confirmed that the nHAEA blended nanofibrous scaffolds substantially increased osteo/odontogenic differentiation of DPSCs. Conclusion PCL/nHAEA nanocomposites had a noticeable effect on the odontogenic differentiation of DPSCs and may help to improve cell-based dentin regeneration therapies in the future. |
| first_indexed | 2024-04-11T06:56:46Z |
| format | Article |
| id | doaj.art-75738601d22f47969291069e9ccc5f7c |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-11T06:56:46Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-75738601d22f47969291069e9ccc5f7c2022-12-22T04:39:01ZengBMCBMC Oral Health1472-68312022-11-0122111310.1186/s12903-022-02527-1Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cellsEhsaneh Azaryan0Mohammad Yahya Hanafi-Bojd1Esmat Alemzadeh2Fariba Emadian Razavi3Mohsen Naseri4Student Research Committee, Birjand University of Medical SciencesCellular and Molecular Research Center, Birjand University of Medical SciencesInfectious Diseases Research Center, Birjand University of Medical SciencesDental Research Center, Faculty of Dentistry, Birjand University of Medical SciencesCellular and Molecular Research Center, Department of Molecular Medicine, Birjand University of Medical SciencesAbstract Purpose The green synthesis of nanoparticles has recently opened up a new route in material production. The aim of this study was to evaluate the effect of nanohydroxyapatite (nHA) synthesized from Elaeagnus angustifolia (EA) extract in polycaprolactone (PCL) nanofibers (PCL/nHAEA) to odontogenic differentiation of dental pulp stem cells (DPSCs) and their potential applications for dentin tissue engineering. Methods Green synthesis of nHA via EA extract (nHAEA) was done by the sol–gel technique. Then electrospun nanocomposites containing of PCL blended with nHA (P/nHA) and nHAEA (P/nHAEA) were fabricated, and the characterization was evaluated via X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and the contact angle. The morphology of nanofibers and the cell adhesion capacity of DPSCs on nanofibers were evaluated using SEM. Cytocompatibility was assessed by MTT. Osteo/odontogenic differentiation ability of the nanocomposites were assessed using alkaline phosphatase (ALP) activity, alizarin red S (ARS) staining, and quantitative real-time polymerase chain reaction (qPCR) technique. Results Viability and adhesion capacity of DPSCs were higher on P/nHAEA nanofibers than PCL and P/nHA nanofibers. ARS assay, ALP activity, and qPCR analysis findings confirmed that the nHAEA blended nanofibrous scaffolds substantially increased osteo/odontogenic differentiation of DPSCs. Conclusion PCL/nHAEA nanocomposites had a noticeable effect on the odontogenic differentiation of DPSCs and may help to improve cell-based dentin regeneration therapies in the future.https://doi.org/10.1186/s12903-022-02527-1Tissue engineeringHydroxyapatiteStem cellElaeagnus angustifoliaDentin regeneration |
| spellingShingle | Ehsaneh Azaryan Mohammad Yahya Hanafi-Bojd Esmat Alemzadeh Fariba Emadian Razavi Mohsen Naseri Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells BMC Oral Health Tissue engineering Hydroxyapatite Stem cell Elaeagnus angustifolia Dentin regeneration |
| title | Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells |
| title_full | Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells |
| title_fullStr | Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells |
| title_full_unstemmed | Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells |
| title_short | Effect of PCL/nHAEA nanocomposite to osteo/odontogenic differentiation of dental pulp stem cells |
| title_sort | effect of pcl nhaea nanocomposite to osteo odontogenic differentiation of dental pulp stem cells |
| topic | Tissue engineering Hydroxyapatite Stem cell Elaeagnus angustifolia Dentin regeneration |
| url | https://doi.org/10.1186/s12903-022-02527-1 |
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