Jun Dimerization Protein 2 (JDP2) Increases p53 Transactivation by Decreasing MDM2
The AP-1 protein complex primarily consists of several proteins from the c-Fos, c-Jun, activating transcription factor (ATF), and Jun dimerization protein (JDP) families. JDP2 has been shown to interact with the cAMP response element (CRE) site present in many cis-elements of downstream target genes...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/16/5/1000 |
| _version_ | 1797264721700716544 |
|---|---|
| author | Kasey Price William H. Yang Leticia Cardoso Chiung-Min Wang Richard H. Yang Wei-Hsiung Yang |
| author_facet | Kasey Price William H. Yang Leticia Cardoso Chiung-Min Wang Richard H. Yang Wei-Hsiung Yang |
| author_sort | Kasey Price |
| collection | DOAJ |
| description | The AP-1 protein complex primarily consists of several proteins from the c-Fos, c-Jun, activating transcription factor (ATF), and Jun dimerization protein (JDP) families. JDP2 has been shown to interact with the cAMP response element (CRE) site present in many cis-elements of downstream target genes. JDP2 has also demonstrates important roles in cell-cycle regulation, cancer development and progression, inhibition of adipocyte differentiation, and the regulation of antibacterial immunity and bone homeostasis. JDP2 and ATF3 exhibit significant similarity in their C-terminal domains, sharing 60–65% identities. Previous studies have demonstrated that ATF3 is able to influence both the transcriptional activity and p53 stability via a p53-ATF3 interaction. While some studies have shown that JDP2 suppresses p53 transcriptional activity and in turn, p53 represses JDP2 promoter activity, the direct interaction between JDP2 and p53 and the regulatory role of JDP2 in p53 transactivation have not been explored. In the current study, we provide evidence, for the first time, that JDP2 interacts with p53 and regulates p53 transactivation. First, we demonstrated that JDP2 binds to p53 and the C-terminal domain of JDP2 is crucial for the interaction. Second, in p53-null H1299 cells, JDP2 shows a robust increase of p53 transactivation in the presence of p53 using p53 (14X)RE-Luc. Furthermore, JDP2 and ATF3 together additively enhance p53 transactivation in the presence of p53. While JDP2 can increase p53 transactivation in the presence of WT p53, JDP2 fails to enhance transactivation of hotspot mutant p53. Moreover, in CHX chase experiments, we showed that JDP2 slightly enhances p53 stability. Finally, our findings indicate that JDP2 has the ability to reverse MDM2-induced p53 repression, likely due to decreased levels of MDM2 by JDP2. In summary, our results provide evidence that JDP2 directly interacts with p53 and decreases MDM2 levels to enhance p53 transactivation, suggesting that JDP2 is a novel regulator of p53 and MDM2. |
| first_indexed | 2024-04-25T00:33:24Z |
| format | Article |
| id | doaj.art-7578a44f00f945a3ad62ac749e6c0ca8 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-04-25T00:33:24Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-7578a44f00f945a3ad62ac749e6c0ca82024-03-12T16:41:09ZengMDPI AGCancers2072-66942024-02-01165100010.3390/cancers16051000Jun Dimerization Protein 2 (JDP2) Increases p53 Transactivation by Decreasing MDM2Kasey Price0William H. Yang1Leticia Cardoso2Chiung-Min Wang3Richard H. Yang4Wei-Hsiung Yang5Department of Biomedical Sciences, School of Medicine, Mercer University, Savannah, GA 31404, USADepartment of Biomedical Sciences, School of Medicine, Mercer University, Savannah, GA 31404, USADepartment of Biomedical Sciences, School of Medicine, Mercer University, Savannah, GA 31404, USADepartment of Biomedical Sciences, School of Medicine, Mercer University, Savannah, GA 31404, USADepartment of Biomedical Sciences, School of Medicine, Mercer University, Savannah, GA 31404, USADepartment of Biomedical Sciences, School of Medicine, Mercer University, Savannah, GA 31404, USAThe AP-1 protein complex primarily consists of several proteins from the c-Fos, c-Jun, activating transcription factor (ATF), and Jun dimerization protein (JDP) families. JDP2 has been shown to interact with the cAMP response element (CRE) site present in many cis-elements of downstream target genes. JDP2 has also demonstrates important roles in cell-cycle regulation, cancer development and progression, inhibition of adipocyte differentiation, and the regulation of antibacterial immunity and bone homeostasis. JDP2 and ATF3 exhibit significant similarity in their C-terminal domains, sharing 60–65% identities. Previous studies have demonstrated that ATF3 is able to influence both the transcriptional activity and p53 stability via a p53-ATF3 interaction. While some studies have shown that JDP2 suppresses p53 transcriptional activity and in turn, p53 represses JDP2 promoter activity, the direct interaction between JDP2 and p53 and the regulatory role of JDP2 in p53 transactivation have not been explored. In the current study, we provide evidence, for the first time, that JDP2 interacts with p53 and regulates p53 transactivation. First, we demonstrated that JDP2 binds to p53 and the C-terminal domain of JDP2 is crucial for the interaction. Second, in p53-null H1299 cells, JDP2 shows a robust increase of p53 transactivation in the presence of p53 using p53 (14X)RE-Luc. Furthermore, JDP2 and ATF3 together additively enhance p53 transactivation in the presence of p53. While JDP2 can increase p53 transactivation in the presence of WT p53, JDP2 fails to enhance transactivation of hotspot mutant p53. Moreover, in CHX chase experiments, we showed that JDP2 slightly enhances p53 stability. Finally, our findings indicate that JDP2 has the ability to reverse MDM2-induced p53 repression, likely due to decreased levels of MDM2 by JDP2. In summary, our results provide evidence that JDP2 directly interacts with p53 and decreases MDM2 levels to enhance p53 transactivation, suggesting that JDP2 is a novel regulator of p53 and MDM2.https://www.mdpi.com/2072-6694/16/5/1000p53JDP2MDM2ATF3transcriptional activity |
| spellingShingle | Kasey Price William H. Yang Leticia Cardoso Chiung-Min Wang Richard H. Yang Wei-Hsiung Yang Jun Dimerization Protein 2 (JDP2) Increases p53 Transactivation by Decreasing MDM2 Cancers p53 JDP2 MDM2 ATF3 transcriptional activity |
| title | Jun Dimerization Protein 2 (JDP2) Increases p53 Transactivation by Decreasing MDM2 |
| title_full | Jun Dimerization Protein 2 (JDP2) Increases p53 Transactivation by Decreasing MDM2 |
| title_fullStr | Jun Dimerization Protein 2 (JDP2) Increases p53 Transactivation by Decreasing MDM2 |
| title_full_unstemmed | Jun Dimerization Protein 2 (JDP2) Increases p53 Transactivation by Decreasing MDM2 |
| title_short | Jun Dimerization Protein 2 (JDP2) Increases p53 Transactivation by Decreasing MDM2 |
| title_sort | jun dimerization protein 2 jdp2 increases p53 transactivation by decreasing mdm2 |
| topic | p53 JDP2 MDM2 ATF3 transcriptional activity |
| url | https://www.mdpi.com/2072-6694/16/5/1000 |
| work_keys_str_mv | AT kaseyprice jundimerizationprotein2jdp2increasesp53transactivationbydecreasingmdm2 AT williamhyang jundimerizationprotein2jdp2increasesp53transactivationbydecreasingmdm2 AT leticiacardoso jundimerizationprotein2jdp2increasesp53transactivationbydecreasingmdm2 AT chiungminwang jundimerizationprotein2jdp2increasesp53transactivationbydecreasingmdm2 AT richardhyang jundimerizationprotein2jdp2increasesp53transactivationbydecreasingmdm2 AT weihsiungyang jundimerizationprotein2jdp2increasesp53transactivationbydecreasingmdm2 |