Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responses
Accumulating evidence support an important role for endogenous bystander dendritic cells (DCs) in the efficiency of autologous patient-derived DC-vaccines, as bystander DCs take up material from vaccine-DCs, migrate to draining lymph node and initiate antitumor T-cell responses. We examined the poss...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-03-01
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| Series: | OncoImmunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/2162402X.2017.1395126 |
| _version_ | 1818155104315899904 |
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| author | Grammatiki Fotaki Chuan Jin Mohanraj Ramachandran Iliana Kyriaki Kerzeli Alex Karlsson-Parra Di Yu Magnus Essand |
| author_facet | Grammatiki Fotaki Chuan Jin Mohanraj Ramachandran Iliana Kyriaki Kerzeli Alex Karlsson-Parra Di Yu Magnus Essand |
| author_sort | Grammatiki Fotaki |
| collection | DOAJ |
| description | Accumulating evidence support an important role for endogenous bystander dendritic cells (DCs) in the efficiency of autologous patient-derived DC-vaccines, as bystander DCs take up material from vaccine-DCs, migrate to draining lymph node and initiate antitumor T-cell responses. We examined the possibility of using allogeneic DCs as vaccine-DCs to activate bystander immune cells and promote antigen-specific T-cell responses. We demonstrate that human DCs matured with polyI:C, R848 and IFN-γ (denoted COMBIG) in combination with an infection-enhanced adenovirus vector (denoted Ad5M) exhibit a pro-inflammatory state. COMBIG/Ad5M-matured allogeneic DCs (alloDCs) efficiently activated T-cells and NK-cells in allogeneic co-culture experiments. The secretion of immunostimulatory factors during the co-culture promoted the maturation of bystander-DCs, which efficiently cross-presented a model-antigen to activate antigen-specific CD8+ T-cells in vitro. We propose that alloDCs, in combination with Ad5M as loading vehicle, may be a cost-effective and logistically simplified DC vaccination strategy to induce anti-tumor immune responses in cancer patients. |
| first_indexed | 2024-12-11T14:37:06Z |
| format | Article |
| id | doaj.art-757ca405ec724cd7902b3108800a9db2 |
| institution | Directory Open Access Journal |
| issn | 2162-402X |
| language | English |
| last_indexed | 2024-12-11T14:37:06Z |
| publishDate | 2018-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | OncoImmunology |
| spelling | doaj.art-757ca405ec724cd7902b3108800a9db22022-12-22T01:02:08ZengTaylor & Francis GroupOncoImmunology2162-402X2018-03-017310.1080/2162402X.2017.13951261395126Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responsesGrammatiki Fotaki0Chuan Jin1Mohanraj Ramachandran2Iliana Kyriaki Kerzeli3Alex Karlsson-Parra4Di Yu5Magnus Essand6Uppsala UniversityUppsala UniversityUppsala UniversityUppsala UniversityUppsala UniversityUppsala UniversityUppsala UniversityAccumulating evidence support an important role for endogenous bystander dendritic cells (DCs) in the efficiency of autologous patient-derived DC-vaccines, as bystander DCs take up material from vaccine-DCs, migrate to draining lymph node and initiate antitumor T-cell responses. We examined the possibility of using allogeneic DCs as vaccine-DCs to activate bystander immune cells and promote antigen-specific T-cell responses. We demonstrate that human DCs matured with polyI:C, R848 and IFN-γ (denoted COMBIG) in combination with an infection-enhanced adenovirus vector (denoted Ad5M) exhibit a pro-inflammatory state. COMBIG/Ad5M-matured allogeneic DCs (alloDCs) efficiently activated T-cells and NK-cells in allogeneic co-culture experiments. The secretion of immunostimulatory factors during the co-culture promoted the maturation of bystander-DCs, which efficiently cross-presented a model-antigen to activate antigen-specific CD8+ T-cells in vitro. We propose that alloDCs, in combination with Ad5M as loading vehicle, may be a cost-effective and logistically simplified DC vaccination strategy to induce anti-tumor immune responses in cancer patients.http://dx.doi.org/10.1080/2162402X.2017.1395126allogeneic dendritic cellscell-based immunotherapyinnate immune cellscell activation |
| spellingShingle | Grammatiki Fotaki Chuan Jin Mohanraj Ramachandran Iliana Kyriaki Kerzeli Alex Karlsson-Parra Di Yu Magnus Essand Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responses OncoImmunology allogeneic dendritic cells cell-based immunotherapy innate immune cells cell activation |
| title | Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responses |
| title_full | Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responses |
| title_fullStr | Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responses |
| title_full_unstemmed | Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responses |
| title_short | Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responses |
| title_sort | pro inflammatory allogeneic dcs promote activation of bystander immune cells and thereby license antigen specific t cell responses |
| topic | allogeneic dendritic cells cell-based immunotherapy innate immune cells cell activation |
| url | http://dx.doi.org/10.1080/2162402X.2017.1395126 |
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