CD140b and CD73 are markers for human induced pluripotent stem cell‐derived erythropoietin‐producing cells
Renal anemia in chronic kidney disease is treated with recombinant human erythropoietin (rhEPO). However, some patients with anemia do not respond well to rhEPO, emphasizing the need for a more biocompatible EPO. Differentiation protocols for hepatic lineages have been modified to enable production...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-03-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12800 |
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| author | Shogo Nishimoto Tomohiro Mizuno Kazuo Takahashi Fumihiko Nagano Yukio Yuzawa Akira Nishiyama Kenji Osafune Hirofumi Hitomi Tadashi Nagamatsu |
| author_facet | Shogo Nishimoto Tomohiro Mizuno Kazuo Takahashi Fumihiko Nagano Yukio Yuzawa Akira Nishiyama Kenji Osafune Hirofumi Hitomi Tadashi Nagamatsu |
| author_sort | Shogo Nishimoto |
| collection | DOAJ |
| description | Renal anemia in chronic kidney disease is treated with recombinant human erythropoietin (rhEPO). However, some patients with anemia do not respond well to rhEPO, emphasizing the need for a more biocompatible EPO. Differentiation protocols for hepatic lineages have been modified to enable production from human induced pluripotent stem cell (hiPSC)‐derived EPO‐producing cells (EPO cells). However, markers for hiPSC‐EPO cells are lacking, making it difficult to purify hiPSC‐EPO cells and therefore to optimize EPO production and cell counts for transplantation. To address these issues, we investigated whether CD140b and CD73 could be used as markers for hiPSC‐EPO cells. We measured the expression of EPO, CD140b, and CD73 in hiPSC‐EPO cells and the EPO concentration in the cell supernatant by immunohistochemistry and enzyme‐linked immunosorbent assays on culture day 13, revealing that expression levels of CD140b and CD73 are correlated with the level of EPO. In addition, rates of CD140b+ CD73+ cells were observed to be correlated with the concentration of EPO. Thus, our results suggest that CD140b and CD73 may be markers for hiPSC‐EPO cells. |
| first_indexed | 2024-12-12T12:37:37Z |
| format | Article |
| id | doaj.art-757d729b4e104452891a5aacfda045ea |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-12T12:37:37Z |
| publishDate | 2020-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-757d729b4e104452891a5aacfda045ea2022-12-22T00:24:18ZengWileyFEBS Open Bio2211-54632020-03-0110342743310.1002/2211-5463.12800CD140b and CD73 are markers for human induced pluripotent stem cell‐derived erythropoietin‐producing cellsShogo Nishimoto0Tomohiro Mizuno1Kazuo Takahashi2Fumihiko Nagano3Yukio Yuzawa4Akira Nishiyama5Kenji Osafune6Hirofumi Hitomi7Tadashi Nagamatsu8Department of Analytical Pharmacology Meijo University Nagoya JapanDepartment of Analytical Pharmacology Meijo University Nagoya JapanDepartment of Nephrology School of Medicine Fujita Health University Toyoake JapanDepartment of Analytical Pharmacology Meijo University Nagoya JapanDepartment of Nephrology School of Medicine Fujita Health University Toyoake JapanDepartment of Pharmacology Faculty of Medicine Kagawa University JapanDepartment of Cell Growth and Differentiation Center for iPS Cell Research and Application (CiRA) Kyoto University JapanDepartment of iPS Stem Cell Regenerative Medicine Kansai Medical University Osaka JapanDepartment of Analytical Pharmacology Meijo University Nagoya JapanRenal anemia in chronic kidney disease is treated with recombinant human erythropoietin (rhEPO). However, some patients with anemia do not respond well to rhEPO, emphasizing the need for a more biocompatible EPO. Differentiation protocols for hepatic lineages have been modified to enable production from human induced pluripotent stem cell (hiPSC)‐derived EPO‐producing cells (EPO cells). However, markers for hiPSC‐EPO cells are lacking, making it difficult to purify hiPSC‐EPO cells and therefore to optimize EPO production and cell counts for transplantation. To address these issues, we investigated whether CD140b and CD73 could be used as markers for hiPSC‐EPO cells. We measured the expression of EPO, CD140b, and CD73 in hiPSC‐EPO cells and the EPO concentration in the cell supernatant by immunohistochemistry and enzyme‐linked immunosorbent assays on culture day 13, revealing that expression levels of CD140b and CD73 are correlated with the level of EPO. In addition, rates of CD140b+ CD73+ cells were observed to be correlated with the concentration of EPO. Thus, our results suggest that CD140b and CD73 may be markers for hiPSC‐EPO cells.https://doi.org/10.1002/2211-5463.12800CD140bCD73chronic kidney diseaseerythropoietinhuman induced pluripotent stem cells |
| spellingShingle | Shogo Nishimoto Tomohiro Mizuno Kazuo Takahashi Fumihiko Nagano Yukio Yuzawa Akira Nishiyama Kenji Osafune Hirofumi Hitomi Tadashi Nagamatsu CD140b and CD73 are markers for human induced pluripotent stem cell‐derived erythropoietin‐producing cells FEBS Open Bio CD140b CD73 chronic kidney disease erythropoietin human induced pluripotent stem cells |
| title | CD140b and CD73 are markers for human induced pluripotent stem cell‐derived erythropoietin‐producing cells |
| title_full | CD140b and CD73 are markers for human induced pluripotent stem cell‐derived erythropoietin‐producing cells |
| title_fullStr | CD140b and CD73 are markers for human induced pluripotent stem cell‐derived erythropoietin‐producing cells |
| title_full_unstemmed | CD140b and CD73 are markers for human induced pluripotent stem cell‐derived erythropoietin‐producing cells |
| title_short | CD140b and CD73 are markers for human induced pluripotent stem cell‐derived erythropoietin‐producing cells |
| title_sort | cd140b and cd73 are markers for human induced pluripotent stem cell derived erythropoietin producing cells |
| topic | CD140b CD73 chronic kidney disease erythropoietin human induced pluripotent stem cells |
| url | https://doi.org/10.1002/2211-5463.12800 |
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