Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A.
Endo-beta-N-acetylglucosaminidases (ENGases) are dual specificity enzymes with an ability to catalyze hydrolysis and transglycosylation reactions. Recently, these enzymes have become the focus of intense research because of their potential for synthesis of glycopeptides. We have determined the 3D st...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2646837?pdf=render |
| _version_ | 1811277071770451968 |
|---|---|
| author | Jie Yin Lei Li Neil Shaw Yang Li Jing Katherine Song Wenpeng Zhang Chengfeng Xia Rongguang Zhang Andrzej Joachimiak Hou-Cheng Zhang Lai-Xi Wang Zhi-Jie Liu Peng Wang |
| author_facet | Jie Yin Lei Li Neil Shaw Yang Li Jing Katherine Song Wenpeng Zhang Chengfeng Xia Rongguang Zhang Andrzej Joachimiak Hou-Cheng Zhang Lai-Xi Wang Zhi-Jie Liu Peng Wang |
| author_sort | Jie Yin |
| collection | DOAJ |
| description | Endo-beta-N-acetylglucosaminidases (ENGases) are dual specificity enzymes with an ability to catalyze hydrolysis and transglycosylation reactions. Recently, these enzymes have become the focus of intense research because of their potential for synthesis of glycopeptides. We have determined the 3D structures of an ENGase from Arthrobacter protophormiae (Endo-A) in 3 forms, one in native form, one in complex with Man(3)GlcNAc-thiazoline and another in complex with GlcNAc-Asn. The carbohydrate moiety sits above the TIM-barrel in a cleft region surrounded by aromatic residues. The conserved essential catalytic residues - E173, N171 and Y205 are within hydrogen bonding distance of the substrate. W216 and W244 regulate access to the active site during transglycosylation by serving as "gate-keepers". Interestingly, Y299F mutation resulted in a 3 fold increase in the transglycosylation activity. The structure provides insights into the catalytic mechanism of GH85 family of glycoside hydrolases at molecular level and could assist rational engineering of ENGases. |
| first_indexed | 2024-04-13T00:09:10Z |
| format | Article |
| id | doaj.art-75803886700c4f83bcd2687cbdf2eb64 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T00:09:10Z |
| publishDate | 2009-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-75803886700c4f83bcd2687cbdf2eb642022-12-22T03:11:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0143e465810.1371/journal.pone.0004658Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A.Jie YinLei LiNeil ShawYang LiJing Katherine SongWenpeng ZhangChengfeng XiaRongguang ZhangAndrzej JoachimiakHou-Cheng ZhangLai-Xi WangZhi-Jie LiuPeng WangEndo-beta-N-acetylglucosaminidases (ENGases) are dual specificity enzymes with an ability to catalyze hydrolysis and transglycosylation reactions. Recently, these enzymes have become the focus of intense research because of their potential for synthesis of glycopeptides. We have determined the 3D structures of an ENGase from Arthrobacter protophormiae (Endo-A) in 3 forms, one in native form, one in complex with Man(3)GlcNAc-thiazoline and another in complex with GlcNAc-Asn. The carbohydrate moiety sits above the TIM-barrel in a cleft region surrounded by aromatic residues. The conserved essential catalytic residues - E173, N171 and Y205 are within hydrogen bonding distance of the substrate. W216 and W244 regulate access to the active site during transglycosylation by serving as "gate-keepers". Interestingly, Y299F mutation resulted in a 3 fold increase in the transglycosylation activity. The structure provides insights into the catalytic mechanism of GH85 family of glycoside hydrolases at molecular level and could assist rational engineering of ENGases.http://europepmc.org/articles/PMC2646837?pdf=render |
| spellingShingle | Jie Yin Lei Li Neil Shaw Yang Li Jing Katherine Song Wenpeng Zhang Chengfeng Xia Rongguang Zhang Andrzej Joachimiak Hou-Cheng Zhang Lai-Xi Wang Zhi-Jie Liu Peng Wang Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A. PLoS ONE |
| title | Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A. |
| title_full | Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A. |
| title_fullStr | Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A. |
| title_full_unstemmed | Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A. |
| title_short | Structural basis and catalytic mechanism for the dual functional endo-beta-N-acetylglucosaminidase A. |
| title_sort | structural basis and catalytic mechanism for the dual functional endo beta n acetylglucosaminidase a |
| url | http://europepmc.org/articles/PMC2646837?pdf=render |
| work_keys_str_mv | AT jieyin structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT leili structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT neilshaw structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT yangli structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT jingkatherinesong structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT wenpengzhang structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT chengfengxia structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT rongguangzhang structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT andrzejjoachimiak structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT houchengzhang structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT laixiwang structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT zhijieliu structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea AT pengwang structuralbasisandcatalyticmechanismforthedualfunctionalendobetanacetylglucosaminidasea |