Label-free protocol to quantify protein affinity using isothermal titration calorimetry and bio-layer interferometry of a human eIF5-mimic protein

Label-free protocol to quantify protein affinity using isothermal titration calorimetry and bio-layer interferometry of a human eIF5-mimic protein

Summary: eIF5-mimic protein (5MP) controls translation through its interaction with eukaryotic translation initiation factor (eIF) 2 and eIF3 and alters non-AUG translation rates for oncogenes in cancer and repeat expansions in neurodegenerative disease. To precisely evaluate the effect of 5MP mutat...

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Bibliographic Details
Main Authors: Chingakham Ranjit Singh, Rahul Jaiswal, Carlos R. Escalante, Katsura Asano
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Biophysics
Molecular biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004956

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