Histopathology of Colletotrichum sp. in infected mango (Magnifera indica L.) fruits
Postharvest losses as a result of anthracnose infection remain a serious threat to mango producers. Hence, histopathology of mango fruits after being artificially infected with spores of Colletotrichum sp. was investigated. Fruits at the physiologically mature stage were wounded (cut) in the peels a...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade - Faculty of Agriculture, Belgrade
2020-01-01
|
| Series: | Journal of Agricultural Sciences (Belgrade) |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-8109/2020/1450-81092004351O.pdf |
| _version_ | 1819160959999016960 |
|---|---|
| author | Oladele Oluwole O. |
| author_facet | Oladele Oluwole O. |
| author_sort | Oladele Oluwole O. |
| collection | DOAJ |
| description | Postharvest losses as a result of anthracnose infection remain a serious threat to mango producers. Hence, histopathology of mango fruits after being artificially infected with spores of Colletotrichum sp. was investigated. Fruits at the physiologically mature stage were wounded (cut) in the peels and inoculated with a spore suspension of Colletotrichum sp. (8.04 × 103 conidia m1-1 ) and incubated at 28±2ºC for five days to allow pathogen establishment. The infected peel was then carefully cut with a razor blade and dehydrated in series in different grades (50, 70, 80, 90 and 100%) of ethyl alcohol for 1½ hours each. Histopathological studies were carried out on the infected peel tissue excised from inoculated fruits using standard procedures while unwounded peels of fruits that were not artificially inoculated served as control. Sections were examined by light microscopy to observe histopathological differences between the infected and noninfected fruits. Results from this study revealed that only the wounded peel showed symptoms of anthracnose infection as a result of the artificial inoculation, but the unwounded peel showed no disease symptoms. This showed that the fungus infected the mango fruits through the peel wounds. Besides, the disorganization of the cells and the rupture of the cell walls were observed microscopically, thus indicating disease establishment in the infected fruits. Therefore, mango producers should avoid mechanical damage to fruits during harvesting since this work confirms that the fungus infects mango fruits through wounds. |
| first_indexed | 2024-12-22T17:04:44Z |
| format | Article |
| id | doaj.art-75852259402f4afdb5de34309a971f5a |
| institution | Directory Open Access Journal |
| issn | 1450-8109 2406-0968 |
| language | English |
| last_indexed | 2024-12-22T17:04:44Z |
| publishDate | 2020-01-01 |
| publisher | University of Belgrade - Faculty of Agriculture, Belgrade |
| record_format | Article |
| series | Journal of Agricultural Sciences (Belgrade) |
| spelling | doaj.art-75852259402f4afdb5de34309a971f5a2022-12-21T18:19:13ZengUniversity of Belgrade - Faculty of Agriculture, BelgradeJournal of Agricultural Sciences (Belgrade)1450-81092406-09682020-01-0165435135910.2298/JAS2004351O1450-81092004351OHistopathology of Colletotrichum sp. in infected mango (Magnifera indica L.) fruitsOladele Oluwole O.0Federal University of Technology, Department of Biology, Akure, NigeriaPostharvest losses as a result of anthracnose infection remain a serious threat to mango producers. Hence, histopathology of mango fruits after being artificially infected with spores of Colletotrichum sp. was investigated. Fruits at the physiologically mature stage were wounded (cut) in the peels and inoculated with a spore suspension of Colletotrichum sp. (8.04 × 103 conidia m1-1 ) and incubated at 28±2ºC for five days to allow pathogen establishment. The infected peel was then carefully cut with a razor blade and dehydrated in series in different grades (50, 70, 80, 90 and 100%) of ethyl alcohol for 1½ hours each. Histopathological studies were carried out on the infected peel tissue excised from inoculated fruits using standard procedures while unwounded peels of fruits that were not artificially inoculated served as control. Sections were examined by light microscopy to observe histopathological differences between the infected and noninfected fruits. Results from this study revealed that only the wounded peel showed symptoms of anthracnose infection as a result of the artificial inoculation, but the unwounded peel showed no disease symptoms. This showed that the fungus infected the mango fruits through the peel wounds. Besides, the disorganization of the cells and the rupture of the cell walls were observed microscopically, thus indicating disease establishment in the infected fruits. Therefore, mango producers should avoid mechanical damage to fruits during harvesting since this work confirms that the fungus infects mango fruits through wounds.https://scindeks-clanci.ceon.rs/data/pdf/1450-8109/2020/1450-81092004351O.pdfinfection processmango anthracnosewoundsmicroscopyinoculation |
| spellingShingle | Oladele Oluwole O. Histopathology of Colletotrichum sp. in infected mango (Magnifera indica L.) fruits Journal of Agricultural Sciences (Belgrade) infection process mango anthracnose wounds microscopy inoculation |
| title | Histopathology of Colletotrichum sp. in infected mango (Magnifera indica L.) fruits |
| title_full | Histopathology of Colletotrichum sp. in infected mango (Magnifera indica L.) fruits |
| title_fullStr | Histopathology of Colletotrichum sp. in infected mango (Magnifera indica L.) fruits |
| title_full_unstemmed | Histopathology of Colletotrichum sp. in infected mango (Magnifera indica L.) fruits |
| title_short | Histopathology of Colletotrichum sp. in infected mango (Magnifera indica L.) fruits |
| title_sort | histopathology of colletotrichum sp in infected mango magnifera indica l fruits |
| topic | infection process mango anthracnose wounds microscopy inoculation |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-8109/2020/1450-81092004351O.pdf |
| work_keys_str_mv | AT oladeleoluwoleo histopathologyofcolletotrichumspininfectedmangomagniferaindicalfruits |