A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNA
Abstract Background ColoDefense1.0 assay has demonstrated its excellent sensitivity and specificity for early detection of colorectal cancer (CRC) by detecting the methylation levels of SDC2 and SEPT9, while exhibited limitations on relatively large sample capacity required and limited detection thr...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | BMC Gastroenterology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12876-022-02512-6 |
| _version_ | 1797996077802061824 |
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| author | Yanmiao Dai Guodong Zhao Jun Yang Xilang Zhou Shangmin Xiong Xirong Lu Liming Gao Jianfang Wu Zouhua Xu Sujuan Fei Minxue Zheng Hongwei Xu |
| author_facet | Yanmiao Dai Guodong Zhao Jun Yang Xilang Zhou Shangmin Xiong Xirong Lu Liming Gao Jianfang Wu Zouhua Xu Sujuan Fei Minxue Zheng Hongwei Xu |
| author_sort | Yanmiao Dai |
| collection | DOAJ |
| description | Abstract Background ColoDefense1.0 assay has demonstrated its excellent sensitivity and specificity for early detection of colorectal cancer (CRC) by detecting the methylation levels of SDC2 and SEPT9, while exhibited limitations on relatively large sample capacity required and limited detection throughput by applying triplicate PCR reactions for each sample. In this study, ColoDefense1.0 was simplified and optimized into ColoDefense2.0 in a single PCR reaction. Methods A total 529 stool specimens were collected, and 244 CRC patients, 34 patients with advanced adenomas (AA), 64 with small polyps (SP) and 187 control subjects were divided in training and validation cohorts. Methylation levels of SEPT9 and SDC2 were examined by qPCR reactions in triplicate or single. Results The stool DNA quantity stored in preservative buffer at 37 °C up to 7 days exhibited no significant decrease. In the training cohort, when the number of replicates reduced from 3 to 1, the overall performance of ColoDefense2.0 was identical to that of ColoDefense1.0, showing sensitivities of 71.4% for AA and 90.8% for all stage CRC with a specificity of 92.9%. In the validation cohort, sensitivities of SP, AA and CRC using ColoDefense2.0 were 25.0%, 55.0% and 88.2%, increased from 14.1% (20.3%), 40.0% (40.0%) and 79.4% (67.6%) using SDC2 (SEPT9) alone; along with an overall specificity of 90.2%, decreased from 94.1% (95.1%) using SDC2 (SEPT9) alone. Conclusion The simplified ColoDefense test maintained the overall performance while reduced the number of PCR reactions to 1/3, and provided an effective and convenient tool to detect early CRC and precancerous lesions and potentially improve the compliance of screening. |
| first_indexed | 2024-04-11T10:11:50Z |
| format | Article |
| id | doaj.art-758856cf2a5843ef9f3d2856580adb8f |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-04-11T10:11:50Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-758856cf2a5843ef9f3d2856580adb8f2022-12-22T04:30:04ZengBMCBMC Gastroenterology1471-230X2022-10-012211910.1186/s12876-022-02512-6A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNAYanmiao Dai0Guodong Zhao1Jun Yang2Xilang Zhou3Shangmin Xiong4Xirong Lu5Liming Gao6Jianfang Wu7Zouhua Xu8Sujuan Fei9Minxue Zheng10Hongwei Xu11Department of Spleen and Stomach Diseases, Kunshan Hospital of Traditional Chinese MedicineZhejiang University Kunshan Biotechnology Laboratory, Zhejiang University Kunshan Innovation InstituteDepartment of Gastroenterology, Affiliated Hospital of Xuzhou Medical UniversityDepartment of Gastroenterology, Affiliated Hospital of Xuzhou Medical UniversityZhejiang University Kunshan Biotechnology Laboratory, Zhejiang University Kunshan Innovation InstituteDepartment of Spleen and Stomach Diseases, Kunshan Hospital of Traditional Chinese MedicineDepartment of Spleen and Stomach Diseases, Kunshan Hospital of Traditional Chinese MedicineDepartment of Spleen and Stomach Diseases, Kunshan Hospital of Traditional Chinese MedicineDepartment of Spleen and Stomach Diseases, Kunshan Hospital of Traditional Chinese MedicineDepartment of Gastroenterology, Affiliated Hospital of Xuzhou Medical UniversityZhejiang University Kunshan Biotechnology Laboratory, Zhejiang University Kunshan Innovation InstituteDepartment of Spleen and Stomach Diseases, Kunshan Hospital of Traditional Chinese MedicineAbstract Background ColoDefense1.0 assay has demonstrated its excellent sensitivity and specificity for early detection of colorectal cancer (CRC) by detecting the methylation levels of SDC2 and SEPT9, while exhibited limitations on relatively large sample capacity required and limited detection throughput by applying triplicate PCR reactions for each sample. In this study, ColoDefense1.0 was simplified and optimized into ColoDefense2.0 in a single PCR reaction. Methods A total 529 stool specimens were collected, and 244 CRC patients, 34 patients with advanced adenomas (AA), 64 with small polyps (SP) and 187 control subjects were divided in training and validation cohorts. Methylation levels of SEPT9 and SDC2 were examined by qPCR reactions in triplicate or single. Results The stool DNA quantity stored in preservative buffer at 37 °C up to 7 days exhibited no significant decrease. In the training cohort, when the number of replicates reduced from 3 to 1, the overall performance of ColoDefense2.0 was identical to that of ColoDefense1.0, showing sensitivities of 71.4% for AA and 90.8% for all stage CRC with a specificity of 92.9%. In the validation cohort, sensitivities of SP, AA and CRC using ColoDefense2.0 were 25.0%, 55.0% and 88.2%, increased from 14.1% (20.3%), 40.0% (40.0%) and 79.4% (67.6%) using SDC2 (SEPT9) alone; along with an overall specificity of 90.2%, decreased from 94.1% (95.1%) using SDC2 (SEPT9) alone. Conclusion The simplified ColoDefense test maintained the overall performance while reduced the number of PCR reactions to 1/3, and provided an effective and convenient tool to detect early CRC and precancerous lesions and potentially improve the compliance of screening.https://doi.org/10.1186/s12876-022-02512-6Colorectal cancerEarly detectionStoolSimplifiedDNA methylation |
| spellingShingle | Yanmiao Dai Guodong Zhao Jun Yang Xilang Zhou Shangmin Xiong Xirong Lu Liming Gao Jianfang Wu Zouhua Xu Sujuan Fei Minxue Zheng Hongwei Xu A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNA BMC Gastroenterology Colorectal cancer Early detection Stool Simplified DNA methylation |
| title | A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNA |
| title_full | A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNA |
| title_fullStr | A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNA |
| title_full_unstemmed | A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNA |
| title_short | A simplified multiplex methylated DNA testing for early detection of colorectal cancer in stool DNA |
| title_sort | simplified multiplex methylated dna testing for early detection of colorectal cancer in stool dna |
| topic | Colorectal cancer Early detection Stool Simplified DNA methylation |
| url | https://doi.org/10.1186/s12876-022-02512-6 |
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