Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae
Honeybee (Apis mellifera) population is going down across the globe due to honeybee pathogens. This greatly influences the bee-associated commercial food products production. Likewise, mosquitoes are prominent vector responsible for spreading life-threatening human diseases, including malaria and de...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-01-01
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| Series: | Journal of Applied Animal Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09712119.2017.1381105 |
| _version_ | 1818359625969303552 |
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| author | Syed Ishtiaq Anjum Ayesha Haleem Shah Ahmed S.A. El-shakh Imran Ullah Amjad Ullah Asifullah Khan Muhammad Ali Abdul Azeez Khan Adnan Khan |
| author_facet | Syed Ishtiaq Anjum Ayesha Haleem Shah Ahmed S.A. El-shakh Imran Ullah Amjad Ullah Asifullah Khan Muhammad Ali Abdul Azeez Khan Adnan Khan |
| author_sort | Syed Ishtiaq Anjum |
| collection | DOAJ |
| description | Honeybee (Apis mellifera) population is going down across the globe due to honeybee pathogens. This greatly influences the bee-associated commercial food products production. Likewise, mosquitoes are prominent vector responsible for spreading life-threatening human diseases, including malaria and dengue. The plant-based insecticides are a better substitute to the recent control practices of honeybee pathogenic bacteria and mosquito. Here, we performed in vitro screening of Nepeta clarkei Hook. f. (Labiatae) aqueous extracts against three honey bee gut bacterial isolates including Paenibacillus larvae an infamous honeybee bacterial pathogen. The inhibitory zone was produced in the range of 6–14 mm diameters against three honey bee bacterial isolates. Likewise, fourth instars larvae of Culex (Diptera/Culicidae) were also subjected to check the possible larvicidal efficacy of N. clarkei. A normal media supplemented with N. clarkei in different concentrations (0.025% 0.05%, 0.1%, 0.15%, and 0.2%) affected the growth of larvae significantly. The lethal concentration at which 50% of larvae failed to become pupate was found to be 0.1% after 24 h of exposure. Considerable reductions in larval growth and pupal development of mosquito suggested that this plant should be utilized in mosquito control programmes. |
| first_indexed | 2024-12-13T20:47:53Z |
| format | Article |
| id | doaj.art-758cb1cb206b425c834c68a94ee97953 |
| institution | Directory Open Access Journal |
| issn | 0971-2119 0974-1844 |
| language | English |
| last_indexed | 2024-12-13T20:47:53Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj.art-758cb1cb206b425c834c68a94ee979532022-12-21T23:31:56ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442018-01-0146166166610.1080/09712119.2017.13811051381105Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvaeSyed Ishtiaq Anjum0Ayesha Haleem Shah1Ahmed S.A. El-shakh2Imran Ullah3Amjad Ullah4Asifullah Khan5Muhammad Ali6Abdul Azeez Khan7Adnan Khan8Kohat University of Science and TechnologyGomal UniversityUniversity of Bakht AlrudaKohat University of Science and TechnologyKohat University of Science and TechnologyAbdul Wali Khan University MardanKarakoram International UniversityKohat University of Science and TechnologyKohat University of Science and TechnologyHoneybee (Apis mellifera) population is going down across the globe due to honeybee pathogens. This greatly influences the bee-associated commercial food products production. Likewise, mosquitoes are prominent vector responsible for spreading life-threatening human diseases, including malaria and dengue. The plant-based insecticides are a better substitute to the recent control practices of honeybee pathogenic bacteria and mosquito. Here, we performed in vitro screening of Nepeta clarkei Hook. f. (Labiatae) aqueous extracts against three honey bee gut bacterial isolates including Paenibacillus larvae an infamous honeybee bacterial pathogen. The inhibitory zone was produced in the range of 6–14 mm diameters against three honey bee bacterial isolates. Likewise, fourth instars larvae of Culex (Diptera/Culicidae) were also subjected to check the possible larvicidal efficacy of N. clarkei. A normal media supplemented with N. clarkei in different concentrations (0.025% 0.05%, 0.1%, 0.15%, and 0.2%) affected the growth of larvae significantly. The lethal concentration at which 50% of larvae failed to become pupate was found to be 0.1% after 24 h of exposure. Considerable reductions in larval growth and pupal development of mosquito suggested that this plant should be utilized in mosquito control programmes.http://dx.doi.org/10.1080/09712119.2017.1381105MosquitohoneybeePeanibacillus larvaeNepeta clarkeilethal concentration |
| spellingShingle | Syed Ishtiaq Anjum Ayesha Haleem Shah Ahmed S.A. El-shakh Imran Ullah Amjad Ullah Asifullah Khan Muhammad Ali Abdul Azeez Khan Adnan Khan Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae Journal of Applied Animal Research Mosquito honeybee Peanibacillus larvae Nepeta clarkei lethal concentration |
| title | Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae |
| title_full | Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae |
| title_fullStr | Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae |
| title_full_unstemmed | Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae |
| title_short | Use of Nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae |
| title_sort | use of nepeta clarkei extracts for controlling honey bee pathogenic bacteria and mosquito larvae |
| topic | Mosquito honeybee Peanibacillus larvae Nepeta clarkei lethal concentration |
| url | http://dx.doi.org/10.1080/09712119.2017.1381105 |
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