MESENCHYMAL STROMAL CELLS IN CORRECTION OF NEUROLOGICAL DEFICIT AND MORPHOLOGICAL CHANGES IN THE RAT MODEL OF FOCAL CEREBRAL LESIONS INDUCED BY VENOUS BLOOD FLOW DISORDER
The paper presents the results of assessing the effect of bone marrow-derived mesenchymal stromal cells (MSCs) on the severity of neurological deficit and the brain morphology and function in a model of focal lesions induced by a venous blood flow disorder. Cerebral lesions were induced in Wistar ra...
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2012-05-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/947 |
| _version_ | 1797237691618689024 |
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| author | E. Ya. Shevela I. A. Vasiljev V. V. Stupak E. V. Polovnikov A. G. Samokhin G. I. Okladnikov D. V. Morozov V. A. Golovnyov E. P. Chernukh |
| author_facet | E. Ya. Shevela I. A. Vasiljev V. V. Stupak E. V. Polovnikov A. G. Samokhin G. I. Okladnikov D. V. Morozov V. A. Golovnyov E. P. Chernukh |
| author_sort | E. Ya. Shevela |
| collection | DOAJ |
| description | The paper presents the results of assessing the effect of bone marrow-derived mesenchymal stromal cells (MSCs) on the severity of neurological deficit and the brain morphology and function in a model of focal lesions induced by a venous blood flow disorder. Cerebral lesions were induced in Wistar rats by coagulation of the superior sagittal sinus followed by coagulation of cortical veins in the left parietotemporal region. MSCs were injected intravenously on days 1 and 7, and the dynamics of neurological disorders and morphological changes were assessed at 7, 14 and 21 days in comparison to controls. MSCs infusion was accompanied by a significant reduction. in neurological deficit, the most pronounced with MSCs injections on day 1. Morphological investigation of the damaged region have shown that the administration of MSCs led to a decrease in the area of necrosis, stimulation of angiogenesis, and improvement of structural and cellular parameters as compared to the control group. As a result, by day 21 the area of necrosis in animals with MSC transplantation was replaced by glial-mesodermal scar, whereas the scar formation in control animals was less pronounced, and in some cases was accompanied by cystic transformation. Transplantation of MSCs has a positive effect on neurological and morphofunctional recovery of the brain in experimental animal models of cerebral venous circulation disorders. |
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| format | Article |
| id | doaj.art-758f274b9f554fd38742e040bd70a5cb |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:23:46Z |
| publishDate | 2012-05-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-758f274b9f554fd38742e040bd70a5cb2024-03-28T07:49:04ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962012-05-0103(2)332336893MESENCHYMAL STROMAL CELLS IN CORRECTION OF NEUROLOGICAL DEFICIT AND MORPHOLOGICAL CHANGES IN THE RAT MODEL OF FOCAL CEREBRAL LESIONS INDUCED BY VENOUS BLOOD FLOW DISORDERE. Ya. Shevela0I. A. Vasiljev1V. V. Stupak2E. V. Polovnikov3A. G. Samokhin4G. I. Okladnikov5D. V. Morozov6V. A. Golovnyov7E. P. Chernukh8Research Institute of Clinical ImmunologyNovosibirsk Research Institute of Traumatology and OrthopaedicsNovosibirsk Research Institute of Traumatology and OrthopaedicsNovosibirsk Research Institute of Traumatology and OrthopaedicsNovosibirsk Research Institute of Traumatology and OrthopaedicsNovosibirsk Research Institute of Traumatology and OrthopaedicsCity Clinical Hospital N 1Novosibirsk State Medical UniversityResearch Institute of Clinical ImmunologyThe paper presents the results of assessing the effect of bone marrow-derived mesenchymal stromal cells (MSCs) on the severity of neurological deficit and the brain morphology and function in a model of focal lesions induced by a venous blood flow disorder. Cerebral lesions were induced in Wistar rats by coagulation of the superior sagittal sinus followed by coagulation of cortical veins in the left parietotemporal region. MSCs were injected intravenously on days 1 and 7, and the dynamics of neurological disorders and morphological changes were assessed at 7, 14 and 21 days in comparison to controls. MSCs infusion was accompanied by a significant reduction. in neurological deficit, the most pronounced with MSCs injections on day 1. Morphological investigation of the damaged region have shown that the administration of MSCs led to a decrease in the area of necrosis, stimulation of angiogenesis, and improvement of structural and cellular parameters as compared to the control group. As a result, by day 21 the area of necrosis in animals with MSC transplantation was replaced by glial-mesodermal scar, whereas the scar formation in control animals was less pronounced, and in some cases was accompanied by cystic transformation. Transplantation of MSCs has a positive effect on neurological and morphofunctional recovery of the brain in experimental animal models of cerebral venous circulation disorders.https://www.actabiomedica.ru/jour/article/view/947mscsvenous blood flow disordermorphologic and functional changesneurological deficit |
| spellingShingle | E. Ya. Shevela I. A. Vasiljev V. V. Stupak E. V. Polovnikov A. G. Samokhin G. I. Okladnikov D. V. Morozov V. A. Golovnyov E. P. Chernukh MESENCHYMAL STROMAL CELLS IN CORRECTION OF NEUROLOGICAL DEFICIT AND MORPHOLOGICAL CHANGES IN THE RAT MODEL OF FOCAL CEREBRAL LESIONS INDUCED BY VENOUS BLOOD FLOW DISORDER Acta Biomedica Scientifica mscs venous blood flow disorder morphologic and functional changes neurological deficit |
| title | MESENCHYMAL STROMAL CELLS IN CORRECTION OF NEUROLOGICAL DEFICIT AND MORPHOLOGICAL CHANGES IN THE RAT MODEL OF FOCAL CEREBRAL LESIONS INDUCED BY VENOUS BLOOD FLOW DISORDER |
| title_full | MESENCHYMAL STROMAL CELLS IN CORRECTION OF NEUROLOGICAL DEFICIT AND MORPHOLOGICAL CHANGES IN THE RAT MODEL OF FOCAL CEREBRAL LESIONS INDUCED BY VENOUS BLOOD FLOW DISORDER |
| title_fullStr | MESENCHYMAL STROMAL CELLS IN CORRECTION OF NEUROLOGICAL DEFICIT AND MORPHOLOGICAL CHANGES IN THE RAT MODEL OF FOCAL CEREBRAL LESIONS INDUCED BY VENOUS BLOOD FLOW DISORDER |
| title_full_unstemmed | MESENCHYMAL STROMAL CELLS IN CORRECTION OF NEUROLOGICAL DEFICIT AND MORPHOLOGICAL CHANGES IN THE RAT MODEL OF FOCAL CEREBRAL LESIONS INDUCED BY VENOUS BLOOD FLOW DISORDER |
| title_short | MESENCHYMAL STROMAL CELLS IN CORRECTION OF NEUROLOGICAL DEFICIT AND MORPHOLOGICAL CHANGES IN THE RAT MODEL OF FOCAL CEREBRAL LESIONS INDUCED BY VENOUS BLOOD FLOW DISORDER |
| title_sort | mesenchymal stromal cells in correction of neurological deficit and morphological changes in the rat model of focal cerebral lesions induced by venous blood flow disorder |
| topic | mscs venous blood flow disorder morphologic and functional changes neurological deficit |
| url | https://www.actabiomedica.ru/jour/article/view/947 |
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