The expression and significance of feces cyclooxygensae-2 mRNA in colorectal cancer and colorectal adenomas
Background/Aim: This study aims to explore the expression and significance of feces cyclooxygensae-2 (COX-2) mRNA in colorectal cancer and colorectal adenomas. Materials and Methods: The expression of feces COX-2 mRNA in colorectal cancer (n = 28), colorectal adenomas (n = 54), and normal control gr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | The Saudi Journal of Gastroenterology |
| Subjects: | |
| Online Access: | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2017;volume=23;issue=1;spage=28;epage=33;aulast=Li |
| _version_ | 1819172082364186624 |
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| author | Xiaofeng Li Lixia Kong Suhuan Liao Jing Lu Lin Ma Xiaohua Long |
| author_facet | Xiaofeng Li Lixia Kong Suhuan Liao Jing Lu Lin Ma Xiaohua Long |
| author_sort | Xiaofeng Li |
| collection | DOAJ |
| description | Background/Aim: This study aims to explore the expression and significance of feces cyclooxygensae-2 (COX-2) mRNA in colorectal cancer and colorectal adenomas. Materials and Methods: The expression of feces COX-2 mRNA in colorectal cancer (n = 28), colorectal adenomas (n = 54), and normal control group (n = 11) were examined by reverse transcriptase polymerase chain reaction (RT-PCR). The positive rate of fecal occult blood test (FOBT) were detected in colorectal cancer (n = 30), colorectal adenomas (n = 56), and normal control group (n = 11); the sensitivity of the two methods was also compared. Results: The positive rate of feces COX-2 mRNA in colorectal cancer was 82.1% (25/28), which was significantly higher than colorectal adenomas 59.3% (32/54), and normal tissues 18.2% (2/11), the difference being significant between the three groups (χ2= 13.842,P= 0.001). The positive rate of FOBT in colorectal cancer was 73.3% (10/30), which was significantly higher than colorectal adenomas 10.7% (6/56) and normal tissues 9.1% (1/11), the difference being significant between these three groups (χ2= 7.525,P= 0.023). There was no significant association between feces COX-2 expression and various clinical pathological features of colorectal cancer and colorectal adenomas (P > 0.05). The sensitivity of the RT-PCR method is higher than FOBT, however, the specificity of FOBT is slightly higher than RT-PCR. Conclusions: High expression of feces COX-2 mRNA in colorectal adenomas and colorectal cancer is a common event; it is an early event in the development of colorectal adenomas to colorectal cancer. Feces COX-2 mRNA has a high sensitivity for detect colorectal cancer; combination with FOBT will be the best alternative. Feces COX-2 can be potentially used in the early diagnosis and screening of colorectal cancer. |
| first_indexed | 2024-12-22T20:01:32Z |
| format | Article |
| id | doaj.art-75933b7251c0402e810f98fa20340183 |
| institution | Directory Open Access Journal |
| issn | 1319-3767 1998-4049 |
| language | English |
| last_indexed | 2024-12-22T20:01:32Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | The Saudi Journal of Gastroenterology |
| spelling | doaj.art-75933b7251c0402e810f98fa203401832022-12-21T18:14:16ZengWolters Kluwer Medknow PublicationsThe Saudi Journal of Gastroenterology1319-37671998-40492017-01-01231283310.4103/1319-3767.199112The expression and significance of feces cyclooxygensae-2 mRNA in colorectal cancer and colorectal adenomasXiaofeng LiLixia KongSuhuan LiaoJing LuLin MaXiaohua LongBackground/Aim: This study aims to explore the expression and significance of feces cyclooxygensae-2 (COX-2) mRNA in colorectal cancer and colorectal adenomas. Materials and Methods: The expression of feces COX-2 mRNA in colorectal cancer (n = 28), colorectal adenomas (n = 54), and normal control group (n = 11) were examined by reverse transcriptase polymerase chain reaction (RT-PCR). The positive rate of fecal occult blood test (FOBT) were detected in colorectal cancer (n = 30), colorectal adenomas (n = 56), and normal control group (n = 11); the sensitivity of the two methods was also compared. Results: The positive rate of feces COX-2 mRNA in colorectal cancer was 82.1% (25/28), which was significantly higher than colorectal adenomas 59.3% (32/54), and normal tissues 18.2% (2/11), the difference being significant between the three groups (χ2= 13.842,P= 0.001). The positive rate of FOBT in colorectal cancer was 73.3% (10/30), which was significantly higher than colorectal adenomas 10.7% (6/56) and normal tissues 9.1% (1/11), the difference being significant between these three groups (χ2= 7.525,P= 0.023). There was no significant association between feces COX-2 expression and various clinical pathological features of colorectal cancer and colorectal adenomas (P > 0.05). The sensitivity of the RT-PCR method is higher than FOBT, however, the specificity of FOBT is slightly higher than RT-PCR. Conclusions: High expression of feces COX-2 mRNA in colorectal adenomas and colorectal cancer is a common event; it is an early event in the development of colorectal adenomas to colorectal cancer. Feces COX-2 mRNA has a high sensitivity for detect colorectal cancer; combination with FOBT will be the best alternative. Feces COX-2 can be potentially used in the early diagnosis and screening of colorectal cancer.http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2017;volume=23;issue=1;spage=28;epage=33;aulast=LiColorectal adenomascolorectal cancerCOX-2 mRNAfecesRT-PCR |
| spellingShingle | Xiaofeng Li Lixia Kong Suhuan Liao Jing Lu Lin Ma Xiaohua Long The expression and significance of feces cyclooxygensae-2 mRNA in colorectal cancer and colorectal adenomas The Saudi Journal of Gastroenterology Colorectal adenomas colorectal cancer COX-2 mRNA feces RT-PCR |
| title | The expression and significance of feces cyclooxygensae-2 mRNA in colorectal cancer and colorectal adenomas |
| title_full | The expression and significance of feces cyclooxygensae-2 mRNA in colorectal cancer and colorectal adenomas |
| title_fullStr | The expression and significance of feces cyclooxygensae-2 mRNA in colorectal cancer and colorectal adenomas |
| title_full_unstemmed | The expression and significance of feces cyclooxygensae-2 mRNA in colorectal cancer and colorectal adenomas |
| title_short | The expression and significance of feces cyclooxygensae-2 mRNA in colorectal cancer and colorectal adenomas |
| title_sort | expression and significance of feces cyclooxygensae 2 mrna in colorectal cancer and colorectal adenomas |
| topic | Colorectal adenomas colorectal cancer COX-2 mRNA feces RT-PCR |
| url | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2017;volume=23;issue=1;spage=28;epage=33;aulast=Li |
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