Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication
Porcine sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag prot...
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/12/4386 |
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| author | Tingting Zhao Li Cui Xiangqian Yu Zhonghai Zhang Qi Chen Xiuguo Hua |
| author_facet | Tingting Zhao Li Cui Xiangqian Yu Zhonghai Zhang Qi Chen Xiuguo Hua |
| author_sort | Tingting Zhao |
| collection | DOAJ |
| description | Porcine sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag proteomics analysis to investigate the differentially expressed proteins (DEPs) in PSV-infected pig kidney (PK)-15 cells and explored the interactions between PSV and host cells. Here we mapped 181 DEPs, including 59 up-regulated and 122 down-regulated DEPs. Among them, osteopontin (SPP1), induced protein with tetratricopeptide repeats 5 (IFIT5), ISG15 ubiquitin-like modifier (ISG15), vinculin (VCL), and syndecan-1 (SDC1) were verified significantly changed using RT-qPCR. Additionally, overexpression of SDC1 promoted PSV viral protein (VP)1 synthesis and virus titer, and silencing of SDC1 revealed the opposite results. Our findings show that SDC1 is a novel host protein and plays crucial roles in regulating PSV replication. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:01:15Z |
| publishDate | 2020-06-01 |
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| spelling | doaj.art-75947dc4389a43ef93d391fe7004e9202023-11-20T04:25:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012112438610.3390/ijms21124386Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus ReplicationTingting Zhao0Li Cui1Xiangqian Yu2Zhonghai Zhang3Qi Chen4Xiuguo Hua5Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Pudong New Area Center for Animal Disease Control and Prevention, Shanghai 200136, ChinaShanghai Pudong New Area Center for Animal Disease Control and Prevention, Shanghai 200136, ChinaShanghai Animal Disease Control Center, Shanghai 201103, ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaPorcine sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag proteomics analysis to investigate the differentially expressed proteins (DEPs) in PSV-infected pig kidney (PK)-15 cells and explored the interactions between PSV and host cells. Here we mapped 181 DEPs, including 59 up-regulated and 122 down-regulated DEPs. Among them, osteopontin (SPP1), induced protein with tetratricopeptide repeats 5 (IFIT5), ISG15 ubiquitin-like modifier (ISG15), vinculin (VCL), and syndecan-1 (SDC1) were verified significantly changed using RT-qPCR. Additionally, overexpression of SDC1 promoted PSV viral protein (VP)1 synthesis and virus titer, and silencing of SDC1 revealed the opposite results. Our findings show that SDC1 is a novel host protein and plays crucial roles in regulating PSV replication.https://www.mdpi.com/1422-0067/21/12/4386quantitative proteomicstandem mass tagsapelovirusreplication |
| spellingShingle | Tingting Zhao Li Cui Xiangqian Yu Zhonghai Zhang Qi Chen Xiuguo Hua Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication International Journal of Molecular Sciences quantitative proteomics tandem mass tag sapelovirus replication |
| title | Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication |
| title_full | Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication |
| title_fullStr | Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication |
| title_full_unstemmed | Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication |
| title_short | Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication |
| title_sort | proteome analysis reveals syndecan 1 regulates porcine sapelovirus replication |
| topic | quantitative proteomics tandem mass tag sapelovirus replication |
| url | https://www.mdpi.com/1422-0067/21/12/4386 |
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