Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of <i>Lotus japonicus</i> by Scavenging Nitric Oxide
Flooding limits biomass production in agriculture. Leguminous plants, important agricultural crops, use atmospheric dinitrogen gas as nitrogen nutrition by symbiotic nitrogen fixation with rhizobia, but this root-nodule symbiosis is sometimes broken down by flooding of the root system. In this study...
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MDPI AG
2019-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/7/206 |
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| author | Mitsutaka Fukudome Eri Watanabe Ken-ichi Osuki Nahoko Uchi Toshiki Uchiumi |
| author_facet | Mitsutaka Fukudome Eri Watanabe Ken-ichi Osuki Nahoko Uchi Toshiki Uchiumi |
| author_sort | Mitsutaka Fukudome |
| collection | DOAJ |
| description | Flooding limits biomass production in agriculture. Leguminous plants, important agricultural crops, use atmospheric dinitrogen gas as nitrogen nutrition by symbiotic nitrogen fixation with rhizobia, but this root-nodule symbiosis is sometimes broken down by flooding of the root system. In this study, we analyzed the effect of flooding on the symbiotic system of transgenic <i>Lotus japonicus</i> lines which overexpressed class 1 phytoglobin (<i>Glb1</i>) of <i>L. japonicus</i> (<i>LjGlb1-1</i>) or ectopically expressed that of <i>Alnus firma</i> (<i>AfGlb1</i>). In the roots of wild-type plants, flooding increased nitric oxide (NO) level and expression of senescence-related genes and decreased nitrogenase activity; in the roots of transgenic lines, these effects were absent or less pronounced. The decrease of chlorophyll content in leaves and the increase of reactive oxygen species (ROS) in roots and leaves caused by flooding were also suppressed in these lines. These results suggest that increased levels of Glb1 help maintain nodule symbiosis under flooding by scavenging NO and controlling ROS. |
| first_indexed | 2024-03-12T09:05:51Z |
| format | Article |
| id | doaj.art-759878a4172c4bc19438ea498028b557 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T09:05:51Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-759878a4172c4bc19438ea498028b5572023-09-02T15:18:19ZengMDPI AGAntioxidants2076-39212019-07-018720610.3390/antiox8070206antiox8070206Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of <i>Lotus japonicus</i> by Scavenging Nitric OxideMitsutaka Fukudome0Eri Watanabe1Ken-ichi Osuki2Nahoko Uchi3Toshiki Uchiumi4Graduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, JapanGraduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, JapanGraduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, JapanGraduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, JapanGraduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima 890-0065, JapanFlooding limits biomass production in agriculture. Leguminous plants, important agricultural crops, use atmospheric dinitrogen gas as nitrogen nutrition by symbiotic nitrogen fixation with rhizobia, but this root-nodule symbiosis is sometimes broken down by flooding of the root system. In this study, we analyzed the effect of flooding on the symbiotic system of transgenic <i>Lotus japonicus</i> lines which overexpressed class 1 phytoglobin (<i>Glb1</i>) of <i>L. japonicus</i> (<i>LjGlb1-1</i>) or ectopically expressed that of <i>Alnus firma</i> (<i>AfGlb1</i>). In the roots of wild-type plants, flooding increased nitric oxide (NO) level and expression of senescence-related genes and decreased nitrogenase activity; in the roots of transgenic lines, these effects were absent or less pronounced. The decrease of chlorophyll content in leaves and the increase of reactive oxygen species (ROS) in roots and leaves caused by flooding were also suppressed in these lines. These results suggest that increased levels of Glb1 help maintain nodule symbiosis under flooding by scavenging NO and controlling ROS.https://www.mdpi.com/2076-3921/8/7/206floodinghemoglobinhypoxia<i>Lotus japonicus</i><i>Mesorhizobium loti</i>nitric oxidenitrogen fixationROSsymbiosis |
| spellingShingle | Mitsutaka Fukudome Eri Watanabe Ken-ichi Osuki Nahoko Uchi Toshiki Uchiumi Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of <i>Lotus japonicus</i> by Scavenging Nitric Oxide Antioxidants flooding hemoglobin hypoxia <i>Lotus japonicus</i> <i>Mesorhizobium loti</i> nitric oxide nitrogen fixation ROS symbiosis |
| title | Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of <i>Lotus japonicus</i> by Scavenging Nitric Oxide |
| title_full | Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of <i>Lotus japonicus</i> by Scavenging Nitric Oxide |
| title_fullStr | Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of <i>Lotus japonicus</i> by Scavenging Nitric Oxide |
| title_full_unstemmed | Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of <i>Lotus japonicus</i> by Scavenging Nitric Oxide |
| title_short | Ectopic or Over-Expression of Class 1 Phytoglobin Genes Confers Flooding Tolerance to the Root Nodules of <i>Lotus japonicus</i> by Scavenging Nitric Oxide |
| title_sort | ectopic or over expression of class 1 phytoglobin genes confers flooding tolerance to the root nodules of i lotus japonicus i by scavenging nitric oxide |
| topic | flooding hemoglobin hypoxia <i>Lotus japonicus</i> <i>Mesorhizobium loti</i> nitric oxide nitrogen fixation ROS symbiosis |
| url | https://www.mdpi.com/2076-3921/8/7/206 |
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