Improvement of the Seminal Characteristics in Rams Using Agri-Food By-Products Rich in Phytomelatonin
The aim of this study was to evaluate the effect of a phytomelatonin-rich diet, including by-products from the food industry, on ram sperm quality and seminal plasma composition. Melatonin content in several by-products before and after in vitro ruminal and abomasal digestion was determined by HPLC-...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/5/905 |
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| author | Victoria Peña-Delgado Melissa Carvajal-Serna Manuel Fondevila María A. Martín-Cabrejas Yolanda Aguilera Gerardo Álvarez-Rivera José A. Abecia Adriana Casao Rosaura Pérez-Pe |
| author_facet | Victoria Peña-Delgado Melissa Carvajal-Serna Manuel Fondevila María A. Martín-Cabrejas Yolanda Aguilera Gerardo Álvarez-Rivera José A. Abecia Adriana Casao Rosaura Pérez-Pe |
| author_sort | Victoria Peña-Delgado |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effect of a phytomelatonin-rich diet, including by-products from the food industry, on ram sperm quality and seminal plasma composition. Melatonin content in several by-products before and after in vitro ruminal and abomasal digestion was determined by HPLC-ESI-MS/MS. Finally, 20% of a mix of grape pulp with pomegranate and tomato pomaces was included in the rams’ diet, constituting the phytomelatonin-rich diet. Feeding the rams with this diet resulted in an increase in seminal plasma melatonin levels compared with the control group (commercial diet) in the third month of the study. In addition, percentages higher than those in the control group of morphologically normal viable spermatozoa with a low content of reactive oxygen species were observed from the second month onwards. However, the antioxidant effect does not seem to be exerted through the modulation of the antioxidant enzymes since the analysis of the activities of catalase, glutathione reductase and glutathione peroxidase in seminal plasma revealed no significant differences between the two experimental groups. In conclusion, this study reveals, for the first time, that a phytomelatonin-rich diet can improve seminal characteristics in rams. |
| first_indexed | 2024-03-11T07:31:52Z |
| format | Article |
| id | doaj.art-759b222fa0c44d13811554619376d798 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T07:31:52Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-759b222fa0c44d13811554619376d7982023-11-17T07:13:55ZengMDPI AGAnimals2076-26152023-03-0113590510.3390/ani13050905Improvement of the Seminal Characteristics in Rams Using Agri-Food By-Products Rich in PhytomelatoninVictoria Peña-Delgado0Melissa Carvajal-Serna1Manuel Fondevila2María A. Martín-Cabrejas3Yolanda Aguilera4Gerardo Álvarez-Rivera5José A. Abecia6Adriana Casao7Rosaura Pérez-Pe8Grupo BIOFITER-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, SpainGrupo BIOFITER-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, SpainDepartamento de Producción Animal y Ciencia de los Alimentos, Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-CITA, 50013 Zaragoza, SpainDepartamento de de Química Agrícola y Bromatología, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, SpainDepartamento de de Química Agrícola y Bromatología, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, SpainInstituto de Investigación en Ciencias de la Alimentación, Spanish National Research Council (CSIC)-Universidad Autónoma de Madrid (UAM), 28049 Madrid, SpainGrupo BIOFITER-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, SpainGrupo BIOFITER-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, SpainGrupo BIOFITER-Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, SpainThe aim of this study was to evaluate the effect of a phytomelatonin-rich diet, including by-products from the food industry, on ram sperm quality and seminal plasma composition. Melatonin content in several by-products before and after in vitro ruminal and abomasal digestion was determined by HPLC-ESI-MS/MS. Finally, 20% of a mix of grape pulp with pomegranate and tomato pomaces was included in the rams’ diet, constituting the phytomelatonin-rich diet. Feeding the rams with this diet resulted in an increase in seminal plasma melatonin levels compared with the control group (commercial diet) in the third month of the study. In addition, percentages higher than those in the control group of morphologically normal viable spermatozoa with a low content of reactive oxygen species were observed from the second month onwards. However, the antioxidant effect does not seem to be exerted through the modulation of the antioxidant enzymes since the analysis of the activities of catalase, glutathione reductase and glutathione peroxidase in seminal plasma revealed no significant differences between the two experimental groups. In conclusion, this study reveals, for the first time, that a phytomelatonin-rich diet can improve seminal characteristics in rams.https://www.mdpi.com/2076-2615/13/5/905circular economydietmelatoninphytomelatoninramseminal quality |
| spellingShingle | Victoria Peña-Delgado Melissa Carvajal-Serna Manuel Fondevila María A. Martín-Cabrejas Yolanda Aguilera Gerardo Álvarez-Rivera José A. Abecia Adriana Casao Rosaura Pérez-Pe Improvement of the Seminal Characteristics in Rams Using Agri-Food By-Products Rich in Phytomelatonin Animals circular economy diet melatonin phytomelatonin ram seminal quality |
| title | Improvement of the Seminal Characteristics in Rams Using Agri-Food By-Products Rich in Phytomelatonin |
| title_full | Improvement of the Seminal Characteristics in Rams Using Agri-Food By-Products Rich in Phytomelatonin |
| title_fullStr | Improvement of the Seminal Characteristics in Rams Using Agri-Food By-Products Rich in Phytomelatonin |
| title_full_unstemmed | Improvement of the Seminal Characteristics in Rams Using Agri-Food By-Products Rich in Phytomelatonin |
| title_short | Improvement of the Seminal Characteristics in Rams Using Agri-Food By-Products Rich in Phytomelatonin |
| title_sort | improvement of the seminal characteristics in rams using agri food by products rich in phytomelatonin |
| topic | circular economy diet melatonin phytomelatonin ram seminal quality |
| url | https://www.mdpi.com/2076-2615/13/5/905 |
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