Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative Bacteria
The development of novel effective antibacterial agents is crucial due to increasing antibiotic resistance in various bacteria. Poly (alkyl cyanoacrylate) nanoparticles (PACA-NPs) are promising novel antibacterial agents as they have shown antibacterial activity against several Gram-positive and Gra...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/12/8/782 |
| _version_ | 1797408920511184896 |
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| author | Fean Davisunjaya Sarian Kazuki Ando Shota Tsurumi Ryohei Miyashita Koichi Ute Takeshi Ohama |
| author_facet | Fean Davisunjaya Sarian Kazuki Ando Shota Tsurumi Ryohei Miyashita Koichi Ute Takeshi Ohama |
| author_sort | Fean Davisunjaya Sarian |
| collection | DOAJ |
| description | The development of novel effective antibacterial agents is crucial due to increasing antibiotic resistance in various bacteria. Poly (alkyl cyanoacrylate) nanoparticles (PACA-NPs) are promising novel antibacterial agents as they have shown antibacterial activity against several Gram-positive and Gram-negative bacteria. However, the antibacterial mechanism remains unclear. Here, we compared the antibacterial efficacy of ethyl cyanoacrylate nanoparticles (ECA-NPs), isobutyl cyanoacrylate NPs (<i><sup>i</sup></i>BCA-NPs), and ethoxyethyl cyanoacrylate NPs (EECA-NPs) using five Gram-positive and five Gram-negative bacteria. Among these resin nanoparticles, ECA-NPs showed the highest growth inhibitory effect against all the examined bacterial species, and this effect was higher against Gram-positive bacteria than Gram-negative. While <i><sup>i</sup></i>BCA-NP could inhibit the cell growth only in two Gram-positive bacteria, i.e., <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>, it had negligible inhibitory effect against all five Gram-negative bacteria examined. Irrespective of the differences in growth inhibition induced by these three NPs, N-acetyl-L-cysteine (NAC), a well-known reactive oxygen species (ROS) scavenger, efficiently restored growth in all the bacterial strains to that similar to untreated cells. This strongly suggests that the exposure to NPs generates ROS, which mainly induces cell growth inhibition irrespective of the difference in bacterial species and cyanoacrylate NPs used. |
| first_indexed | 2024-03-09T04:06:38Z |
| format | Article |
| id | doaj.art-759cfe85e2ee434da70d4b671446bff5 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T04:06:38Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-759cfe85e2ee434da70d4b671446bff52023-12-03T14:05:40ZengMDPI AGMembranes2077-03752022-08-0112878210.3390/membranes12080782Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative BacteriaFean Davisunjaya Sarian0Kazuki Ando1Shota Tsurumi2Ryohei Miyashita3Koichi Ute4Takeshi Ohama5School of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada, Kami 782-8502, JapanSchool of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada, Kami 782-8502, JapanSchool of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada, Kami 782-8502, JapanDepartment of Applied Chemistry, Tokushima University, 2-1 Minami-Josanjima, Tokushima 770-8506, JapanDepartment of Applied Chemistry, Tokushima University, 2-1 Minami-Josanjima, Tokushima 770-8506, JapanSchool of Environmental Science and Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada, Kami 782-8502, JapanThe development of novel effective antibacterial agents is crucial due to increasing antibiotic resistance in various bacteria. Poly (alkyl cyanoacrylate) nanoparticles (PACA-NPs) are promising novel antibacterial agents as they have shown antibacterial activity against several Gram-positive and Gram-negative bacteria. However, the antibacterial mechanism remains unclear. Here, we compared the antibacterial efficacy of ethyl cyanoacrylate nanoparticles (ECA-NPs), isobutyl cyanoacrylate NPs (<i><sup>i</sup></i>BCA-NPs), and ethoxyethyl cyanoacrylate NPs (EECA-NPs) using five Gram-positive and five Gram-negative bacteria. Among these resin nanoparticles, ECA-NPs showed the highest growth inhibitory effect against all the examined bacterial species, and this effect was higher against Gram-positive bacteria than Gram-negative. While <i><sup>i</sup></i>BCA-NP could inhibit the cell growth only in two Gram-positive bacteria, i.e., <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>, it had negligible inhibitory effect against all five Gram-negative bacteria examined. Irrespective of the differences in growth inhibition induced by these three NPs, N-acetyl-L-cysteine (NAC), a well-known reactive oxygen species (ROS) scavenger, efficiently restored growth in all the bacterial strains to that similar to untreated cells. This strongly suggests that the exposure to NPs generates ROS, which mainly induces cell growth inhibition irrespective of the difference in bacterial species and cyanoacrylate NPs used.https://www.mdpi.com/2077-0375/12/8/782antibacterial agentcyanoacrylate nanoparticlemembrane damagereactive oxygen speciesstress response |
| spellingShingle | Fean Davisunjaya Sarian Kazuki Ando Shota Tsurumi Ryohei Miyashita Koichi Ute Takeshi Ohama Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative Bacteria Membranes antibacterial agent cyanoacrylate nanoparticle membrane damage reactive oxygen species stress response |
| title | Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative Bacteria |
| title_full | Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative Bacteria |
| title_fullStr | Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative Bacteria |
| title_full_unstemmed | Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative Bacteria |
| title_short | Evaluation of the Growth-Inhibitory Spectrum of Three Types of Cyanoacrylate Nanoparticles on Gram-Positive and Gram-Negative Bacteria |
| title_sort | evaluation of the growth inhibitory spectrum of three types of cyanoacrylate nanoparticles on gram positive and gram negative bacteria |
| topic | antibacterial agent cyanoacrylate nanoparticle membrane damage reactive oxygen species stress response |
| url | https://www.mdpi.com/2077-0375/12/8/782 |
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