A Novel Model of <i>Staphylococcus aureus</i>-Induced Lymphoplasmacytic Rhinosinusitis in Rats
Chronic rhinosinusitis (CRS) is characterized by sinonasal mucosal inflammation. <i>Staphylococcus aureus (S. aureus)</i> is associated with severe CRS phenotypes. Different animal models have been proposed to study the association of CRS and <i>S. aureus</i>. However, curren...
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| Format: | Article |
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/6/3336 |
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| author | William Murphy Sha Liu Karen Hon John Finnie George Spyro Bouras Sholeh Feizi Ghais Houtak Gohar Shaghayegh Erich Vyskocil Peter-John Wormald Sarah Vreugde Alkis J. Psaltis |
| author_facet | William Murphy Sha Liu Karen Hon John Finnie George Spyro Bouras Sholeh Feizi Ghais Houtak Gohar Shaghayegh Erich Vyskocil Peter-John Wormald Sarah Vreugde Alkis J. Psaltis |
| author_sort | William Murphy |
| collection | DOAJ |
| description | Chronic rhinosinusitis (CRS) is characterized by sinonasal mucosal inflammation. <i>Staphylococcus aureus (S. aureus)</i> is associated with severe CRS phenotypes. Different animal models have been proposed to study the association of CRS and <i>S. aureus</i>. However, current animal models are expensive due to the use of large animals, have high barriers to ethics approval, or require invasive surgical intervention, necessitating a need for a model that can overcome these limitations. This study aimed at establishing a reliable and efficient rat lymphoplasmacytic inflammatory model for rhinosinusitis. Sprague Dawley rats received a daily intranasal application of 20 μL of saline, <i>S. aureus</i> CI-182 exoprotein (250 μg/mL), or exoprotein CI-182 in combination with <i>S. aureus</i> clinical isolate (CI-908 or CI-913) 10<sup>8</sup> colony-forming unit (CFU)/mL. The rats’ sinuses were harvested at 1 and 2 weeks post-intervention. The CFU and histopathologic examination of inflammation were evaluated. <i>S. aureus</i> clinical isolates CI-908 or CI-913 in combination with the exoprotein (CI-182) had higher CFUs and caused persistently higher inflammation at both the 1 and 2-week post-intervention compared to the exoprotein and saline group. The observed inflammatory cell type was lymphoplasmacytic. This study provided evidence that the combination of a <i>S. aureus</i> exoprotein with <i>S. aureus</i> induces inflammation that persists for a minimum of two weeks post-intervention. This model is the first known animal model to create the lymphoplasmacytic inflammation subtype seen in CRS patients. This offers a cost-effective, accessible, non-invasive, and easy-to-replicate model to study the causes and treatment of such inflammation. |
| first_indexed | 2024-04-24T18:11:24Z |
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| id | doaj.art-759d1b6a5ece4cd6842e00b72a631083 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T18:11:24Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-759d1b6a5ece4cd6842e00b72a6310832024-03-27T13:45:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01256333610.3390/ijms25063336A Novel Model of <i>Staphylococcus aureus</i>-Induced Lymphoplasmacytic Rhinosinusitis in RatsWilliam Murphy0Sha Liu1Karen Hon2John Finnie3George Spyro Bouras4Sholeh Feizi5Ghais Houtak6Gohar Shaghayegh7Erich Vyskocil8Peter-John Wormald9Sarah Vreugde10Alkis J. Psaltis11Department of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDivision of Research and Innovation, University of Adelaide, Adelaide, SA 5005, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaDepartment of Surgery-Otolaryngology Head and Neck Surgery, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville South, SA 5011, AustraliaChronic rhinosinusitis (CRS) is characterized by sinonasal mucosal inflammation. <i>Staphylococcus aureus (S. aureus)</i> is associated with severe CRS phenotypes. Different animal models have been proposed to study the association of CRS and <i>S. aureus</i>. However, current animal models are expensive due to the use of large animals, have high barriers to ethics approval, or require invasive surgical intervention, necessitating a need for a model that can overcome these limitations. This study aimed at establishing a reliable and efficient rat lymphoplasmacytic inflammatory model for rhinosinusitis. Sprague Dawley rats received a daily intranasal application of 20 μL of saline, <i>S. aureus</i> CI-182 exoprotein (250 μg/mL), or exoprotein CI-182 in combination with <i>S. aureus</i> clinical isolate (CI-908 or CI-913) 10<sup>8</sup> colony-forming unit (CFU)/mL. The rats’ sinuses were harvested at 1 and 2 weeks post-intervention. The CFU and histopathologic examination of inflammation were evaluated. <i>S. aureus</i> clinical isolates CI-908 or CI-913 in combination with the exoprotein (CI-182) had higher CFUs and caused persistently higher inflammation at both the 1 and 2-week post-intervention compared to the exoprotein and saline group. The observed inflammatory cell type was lymphoplasmacytic. This study provided evidence that the combination of a <i>S. aureus</i> exoprotein with <i>S. aureus</i> induces inflammation that persists for a minimum of two weeks post-intervention. This model is the first known animal model to create the lymphoplasmacytic inflammation subtype seen in CRS patients. This offers a cost-effective, accessible, non-invasive, and easy-to-replicate model to study the causes and treatment of such inflammation.https://www.mdpi.com/1422-0067/25/6/3336<i>Staphylococcus aureus</i>chronic rhinosinusitislymphoplasmacytic inflammatory responserodent model |
| spellingShingle | William Murphy Sha Liu Karen Hon John Finnie George Spyro Bouras Sholeh Feizi Ghais Houtak Gohar Shaghayegh Erich Vyskocil Peter-John Wormald Sarah Vreugde Alkis J. Psaltis A Novel Model of <i>Staphylococcus aureus</i>-Induced Lymphoplasmacytic Rhinosinusitis in Rats International Journal of Molecular Sciences <i>Staphylococcus aureus</i> chronic rhinosinusitis lymphoplasmacytic inflammatory response rodent model |
| title | A Novel Model of <i>Staphylococcus aureus</i>-Induced Lymphoplasmacytic Rhinosinusitis in Rats |
| title_full | A Novel Model of <i>Staphylococcus aureus</i>-Induced Lymphoplasmacytic Rhinosinusitis in Rats |
| title_fullStr | A Novel Model of <i>Staphylococcus aureus</i>-Induced Lymphoplasmacytic Rhinosinusitis in Rats |
| title_full_unstemmed | A Novel Model of <i>Staphylococcus aureus</i>-Induced Lymphoplasmacytic Rhinosinusitis in Rats |
| title_short | A Novel Model of <i>Staphylococcus aureus</i>-Induced Lymphoplasmacytic Rhinosinusitis in Rats |
| title_sort | novel model of i staphylococcus aureus i induced lymphoplasmacytic rhinosinusitis in rats |
| topic | <i>Staphylococcus aureus</i> chronic rhinosinusitis lymphoplasmacytic inflammatory response rodent model |
| url | https://www.mdpi.com/1422-0067/25/6/3336 |
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