RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determin...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/9082 |
| _version_ | 1797599740670509056 |
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| author | Isabel Padilla-Roji Laura Ruiz-Jiménez Nisrine Bakhat Alejandra Vielba-Fernández Alejandro Pérez-García Dolores Fernández-Ortuño |
| author_facet | Isabel Padilla-Roji Laura Ruiz-Jiménez Nisrine Bakhat Alejandra Vielba-Fernández Alejandro Pérez-García Dolores Fernández-Ortuño |
| author_sort | Isabel Padilla-Roji |
| collection | DOAJ |
| description | Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determined by the presence of haustoria, specialized fungal cells that are responsible for nutrient uptake and molecular dialogue with the host, a fact that undoubtedly complicates their study under laboratory conditions, especially in terms of genetic manipulation. RNA interference (RNAi) is the biological process of suppressing the expression of a target gene through double-stranded RNA that induces mRNA degradation. RNAi technology has revolutionized the study of these obligate biotrophic fungi by enabling the analysis of gene function in these fungal. More importantly, RNAi technology has opened new perspectives for the management of powdery mildew and rust diseases, first through the stable expression of RNAi constructs in transgenic plants and, more recently, through the non-transgenic approach called spray-induced gene silencing (SIGS). In this review, the impact of RNAi technology on the research and management of powdery mildew and rust fungi will be addressed. |
| first_indexed | 2024-03-11T03:38:34Z |
| format | Article |
| id | doaj.art-759f81cf80d14e7182f0cf16b6d0d59e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:38:34Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-759f81cf80d14e7182f0cf16b6d0d59e2023-11-18T01:47:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410908210.3390/ijms24109082RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic FungiIsabel Padilla-Roji0Laura Ruiz-Jiménez1Nisrine Bakhat2Alejandra Vielba-Fernández3Alejandro Pérez-García4Dolores Fernández-Ortuño5Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, SpainDepartamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, SpainDepartamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, SpainDepartamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, SpainDepartamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, SpainDepartamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, SpainPowdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determined by the presence of haustoria, specialized fungal cells that are responsible for nutrient uptake and molecular dialogue with the host, a fact that undoubtedly complicates their study under laboratory conditions, especially in terms of genetic manipulation. RNA interference (RNAi) is the biological process of suppressing the expression of a target gene through double-stranded RNA that induces mRNA degradation. RNAi technology has revolutionized the study of these obligate biotrophic fungi by enabling the analysis of gene function in these fungal. More importantly, RNAi technology has opened new perspectives for the management of powdery mildew and rust diseases, first through the stable expression of RNAi constructs in transgenic plants and, more recently, through the non-transgenic approach called spray-induced gene silencing (SIGS). In this review, the impact of RNAi technology on the research and management of powdery mildew and rust fungi will be addressed.https://www.mdpi.com/1422-0067/24/10/9082RNA interferenceVIGSHIGSATM-HIGSdsRNASIGS |
| spellingShingle | Isabel Padilla-Roji Laura Ruiz-Jiménez Nisrine Bakhat Alejandra Vielba-Fernández Alejandro Pérez-García Dolores Fernández-Ortuño RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi International Journal of Molecular Sciences RNA interference VIGS HIGS ATM-HIGS dsRNA SIGS |
| title | RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi |
| title_full | RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi |
| title_fullStr | RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi |
| title_full_unstemmed | RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi |
| title_short | RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi |
| title_sort | rnai technology a new path for the research and management of obligate biotrophic phytopathogenic fungi |
| topic | RNA interference VIGS HIGS ATM-HIGS dsRNA SIGS |
| url | https://www.mdpi.com/1422-0067/24/10/9082 |
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