A decellularized human corneal scaffold for anterior corneal surface reconstruction
Abstract Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-82678-3 |
| _version_ | 1818681631237472256 |
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| author | Naresh Polisetti Anke Schmid Ursula Schlötzer-Schrehardt Philip Maier Stefan J. Lang Thorsten Steinberg Günther Schlunck Thomas Reinhard |
| author_facet | Naresh Polisetti Anke Schmid Ursula Schlötzer-Schrehardt Philip Maier Stefan J. Lang Thorsten Steinberg Günther Schlunck Thomas Reinhard |
| author_sort | Naresh Polisetti |
| collection | DOAJ |
| description | Abstract Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for transplantation, which may be repopulated by host cells in situ or in vitro. So, the aim of this study was to develop a fast and efficient decellularization method for creating a human corneal extracellular matrix scaffold suitable for repopulation with human cells from the corneal limbus. To decellularize human donor corneas, sodium deoxycholate, deoxyribonuclease I, and dextran were assessed to remove cells and nuclei and to control tissue swelling, respectively. We evaluated the decellularization effects on the ultrastructure, optical, mechanical, and biological properties of the human cornea. Scaffold recellularization was studied using primary human limbal epithelial cells, stromal cells, and melanocytes in vitro and a lamellar transplantation approach ex vivo. Our data strongly suggest that this approach allowed the effective removal of cellular and nuclear material in a very short period of time while preserving extracellular matrix proteins, glycosaminoglycans, tissue structure, and optical transmission properties. In vitro recellularization demonstrated good biocompatibility of the decellularized human cornea and ex vivo transplantation revealed complete epithelialization and stromal repopulation from the host tissue. Thus, the generated decellularized human corneal scaffold could be a promising biological material for anterior corneal reconstruction in the treatment of corneal defects. |
| first_indexed | 2024-12-17T10:06:01Z |
| format | Article |
| id | doaj.art-75a5490fdcfc4ced89020dd19c994886 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T10:06:01Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-75a5490fdcfc4ced89020dd19c9948862022-12-21T21:53:09ZengNature PortfolioScientific Reports2045-23222021-02-0111111510.1038/s41598-021-82678-3A decellularized human corneal scaffold for anterior corneal surface reconstructionNaresh Polisetti0Anke Schmid1Ursula Schlötzer-Schrehardt2Philip Maier3Stefan J. Lang4Thorsten Steinberg5Günther Schlunck6Thomas Reinhard7Eye Center, Medical Center - Faculty of Medicine, University of FreiburgEye Center, Medical Center - Faculty of Medicine, University of FreiburgDepartment of Ophthalmology, University Hospital Erlangen, Friedrich-Alexander-University of Erlangen-NürnbergEye Center, Medical Center - Faculty of Medicine, University of FreiburgEye Center, Medical Center - Faculty of Medicine, University of FreiburgDepartment of Operative Dentistry and Periodontology, Division of Oral Biotechnology, University of FreiburgEye Center, Medical Center - Faculty of Medicine, University of FreiburgEye Center, Medical Center - Faculty of Medicine, University of FreiburgAbstract Allogenic transplants of the cornea are prone to rejection, especially in repetitive transplantation and in scarred or highly vascularized recipient sites. Patients with these ailments would particularly benefit from the possibility to use non-immunogenic decellularized tissue scaffolds for transplantation, which may be repopulated by host cells in situ or in vitro. So, the aim of this study was to develop a fast and efficient decellularization method for creating a human corneal extracellular matrix scaffold suitable for repopulation with human cells from the corneal limbus. To decellularize human donor corneas, sodium deoxycholate, deoxyribonuclease I, and dextran were assessed to remove cells and nuclei and to control tissue swelling, respectively. We evaluated the decellularization effects on the ultrastructure, optical, mechanical, and biological properties of the human cornea. Scaffold recellularization was studied using primary human limbal epithelial cells, stromal cells, and melanocytes in vitro and a lamellar transplantation approach ex vivo. Our data strongly suggest that this approach allowed the effective removal of cellular and nuclear material in a very short period of time while preserving extracellular matrix proteins, glycosaminoglycans, tissue structure, and optical transmission properties. In vitro recellularization demonstrated good biocompatibility of the decellularized human cornea and ex vivo transplantation revealed complete epithelialization and stromal repopulation from the host tissue. Thus, the generated decellularized human corneal scaffold could be a promising biological material for anterior corneal reconstruction in the treatment of corneal defects.https://doi.org/10.1038/s41598-021-82678-3 |
| spellingShingle | Naresh Polisetti Anke Schmid Ursula Schlötzer-Schrehardt Philip Maier Stefan J. Lang Thorsten Steinberg Günther Schlunck Thomas Reinhard A decellularized human corneal scaffold for anterior corneal surface reconstruction Scientific Reports |
| title | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
| title_full | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
| title_fullStr | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
| title_full_unstemmed | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
| title_short | A decellularized human corneal scaffold for anterior corneal surface reconstruction |
| title_sort | decellularized human corneal scaffold for anterior corneal surface reconstruction |
| url | https://doi.org/10.1038/s41598-021-82678-3 |
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