The influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in rats
Physical effort can elicit differential adaptive changes in the tissues of trained versus untrained rats. Proteolytic activity in the extracellular matrix could be engaged in such adaptation due to its influence on the elasticity of tissues. The effects were investigated of a single physical effor...
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Institute of Rural Health
2018-12-01
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| Series: | Annals of Agricultural and Environmental Medicine |
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| Online Access: | http://www.journalssystem.com/aaem/The-influence-of-heavy-physical-effort-on-proteolytic-adaptations-in-skeletal-and-heart-muscle-and-aorta-in-rats-,74315,0,2.html |
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| author | Anna Gilbert-Matusiak Aleksandra Wyczalkowska-Tomasik Małgorzata Zendzian-Piotrowska Bożena Czarkowska-Pączek |
| author_facet | Anna Gilbert-Matusiak Aleksandra Wyczalkowska-Tomasik Małgorzata Zendzian-Piotrowska Bożena Czarkowska-Pączek |
| author_sort | Anna Gilbert-Matusiak |
| collection | DOAJ |
| description | Physical effort can elicit differential adaptive changes in the tissues of trained versus untrained rats. Proteolytic activity in
the extracellular matrix could be engaged in such adaptation due to its influence on the elasticity of tissues. The effects were
investigated of a single physical effort on the activity of elastase, cathepsin K, and plasmin in the skeletal muscles, heart
muscles, and aortas of untrained (UT, n=30) and trained (T, n=30) rats. T rats underwent 6 weeks of endurance training. After
the last training session, T and UT rats were divided randomly into 3 subgroups. Ten rats from each group (Tpre, n=10) and
(UTpre, n=10) were sacrificed. The other 20 rats from each group performed 60 min. of aerobic exercise and were sacrificed
immediately post exercise (T0h, n=10; UT0h, n=10) or 3h later (T3h, n=10; UT3h, n=10). Enzyme activity was measured
fluorometrically. Cathepsin K and plasmin activity increased in the soleus muscles of UT0h versus UTpre, plasmin activity
increased also in UT3h versus UTpre. Elastase, cathepsin K and plasmin activity increased in the heart muscles of T0h and
T3h versus Tpre. No aortic differences were observed. Thus, a single bout of physical effort elicited different responses in
tissues of T versus UT rats. Increased proteolytic enzyme levels in muscles could influence tissue remodeling. Unchanged
aortic cathepsin K levels may help prevent aortic remodeling and neointima formation. |
| first_indexed | 2024-12-10T22:36:11Z |
| format | Article |
| id | doaj.art-75a66b104c214220a9df94e29d9b6d46 |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-10T22:36:11Z |
| publishDate | 2018-12-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-75a66b104c214220a9df94e29d9b6d462022-12-22T01:30:52ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632018-12-0125460560910.26444/aaem/7431574315The influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in ratsAnna Gilbert-Matusiak0Aleksandra Wyczalkowska-Tomasik1Małgorzata Zendzian-Piotrowska2Bożena Czarkowska-Pączek3Department of Clinical Nursing, Medical University, Warsaw, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University, Warsaw, PolandDepartment of Hygiene, Epidemiology and Ergonomics, Medical University, Bialystok, PolandDepartment of Clinical Nursing, Medical University, Warsaw, PolandPhysical effort can elicit differential adaptive changes in the tissues of trained versus untrained rats. Proteolytic activity in the extracellular matrix could be engaged in such adaptation due to its influence on the elasticity of tissues. The effects were investigated of a single physical effort on the activity of elastase, cathepsin K, and plasmin in the skeletal muscles, heart muscles, and aortas of untrained (UT, n=30) and trained (T, n=30) rats. T rats underwent 6 weeks of endurance training. After the last training session, T and UT rats were divided randomly into 3 subgroups. Ten rats from each group (Tpre, n=10) and (UTpre, n=10) were sacrificed. The other 20 rats from each group performed 60 min. of aerobic exercise and were sacrificed immediately post exercise (T0h, n=10; UT0h, n=10) or 3h later (T3h, n=10; UT3h, n=10). Enzyme activity was measured fluorometrically. Cathepsin K and plasmin activity increased in the soleus muscles of UT0h versus UTpre, plasmin activity increased also in UT3h versus UTpre. Elastase, cathepsin K and plasmin activity increased in the heart muscles of T0h and T3h versus Tpre. No aortic differences were observed. Thus, a single bout of physical effort elicited different responses in tissues of T versus UT rats. Increased proteolytic enzyme levels in muscles could influence tissue remodeling. Unchanged aortic cathepsin K levels may help prevent aortic remodeling and neointima formation.http://www.journalssystem.com/aaem/The-influence-of-heavy-physical-effort-on-proteolytic-adaptations-in-skeletal-and-heart-muscle-and-aorta-in-rats-,74315,0,2.htmlphysical effortproteolytic enzymestissue remodeling |
| spellingShingle | Anna Gilbert-Matusiak Aleksandra Wyczalkowska-Tomasik Małgorzata Zendzian-Piotrowska Bożena Czarkowska-Pączek The influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in rats Annals of Agricultural and Environmental Medicine physical effort proteolytic enzymes tissue remodeling |
| title | The influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in rats |
| title_full | The influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in rats |
| title_fullStr | The influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in rats |
| title_full_unstemmed | The influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in rats |
| title_short | The influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in rats |
| title_sort | influence of heavy physical effort on proteolytic adaptations in skeletal and heart muscle and aorta in rats |
| topic | physical effort proteolytic enzymes tissue remodeling |
| url | http://www.journalssystem.com/aaem/The-influence-of-heavy-physical-effort-on-proteolytic-adaptations-in-skeletal-and-heart-muscle-and-aorta-in-rats-,74315,0,2.html |
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