High-level aminoglycoside resistance and distribution of aminoglycoside resistance genes among Enterococcus spp. clinical isolates in Tehran, Iran
Objectives: Enterococci have gained attention during the past decade as important nosocomial pathogens. Their increasing prevalence has been paralleled by the occurrence of multidrug-resistant and high-level aminoglycoside-resistant strains. This study isolated Enterococcus spp. from hospital sample...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-03-01
|
| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716519302061 |
| _version_ | 1818657882878509056 |
|---|---|
| author | V. Sharifzadeh Peyvasti A. Mohabati Mobarez F. Shahcheraghi N. Khoramabadi N. Razaz Rahmati R. Hosseini Doust |
| author_facet | V. Sharifzadeh Peyvasti A. Mohabati Mobarez F. Shahcheraghi N. Khoramabadi N. Razaz Rahmati R. Hosseini Doust |
| author_sort | V. Sharifzadeh Peyvasti |
| collection | DOAJ |
| description | Objectives: Enterococci have gained attention during the past decade as important nosocomial pathogens. Their increasing prevalence has been paralleled by the occurrence of multidrug-resistant and high-level aminoglycoside-resistant strains. This study isolated Enterococcus spp. from hospital samples and determined their antibiotic resistance profile, focusing on aminoglycosides, and associated resistance mechanisms. Methods: A total of 195 enterococci from hospital samples in Tehran were studied. Isolates were identified by biochemical reactions. Antimicrobial resistance was determined by disk diffusion. The vancomycin MIC for vancomycin-resistant isolates was determined by agar dilution. Detection of aminoglycoside resistance genes and intI1 and intI2 gene was performed by PCR. Results: The majority of isolates were Enterococcus faecalis (65.1%), followed by Enterococcus faecium (31.8%), Enterococcus gallinarum (2.6%) and Enterococcus solitarius (0.5%). According to antibiogram results, 42.1% of isolates were high-level gentamicin-resistant (HLGR) and 40.5% were high-level streptomycin-resistant (HLSR). There was a high prevalence of aac(6')-Ie–aph(2'')-Ia (96.3%) among HLGR isolates. ant(6)-Ia and aadA were identified in 93.7% and 64.6% of HLSR isolates, respectively. aph(2ʹʹ)-Ic was detected in 7 isolates (3.6%) and aph(2ʹʹ)-Ib in only 4 isolates (2.1%); no isolates harboured aph(2ʹʹ)-Id, intI1 or intI2. Conclusion: Multidrug resistance was higher among HLGR and HLSR isolates compared with non-HLGR and non-HLSR isolates, which may result in limited treatment options. More than 50% of isolates were susceptible to aminoglycosides, thus correct identification in clinical laboratories and administration of these antibiotics can result in decreased used of antibiotics such as vancomycin and linezolid and help to reduce the emergence of resistance to these drugs. |
| first_indexed | 2024-12-17T03:48:33Z |
| format | Article |
| id | doaj.art-75a975fe66324fb3b61918b51bdf738b |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-17T03:48:33Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-75a975fe66324fb3b61918b51bdf738b2022-12-21T22:04:49ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-03-0120318323High-level aminoglycoside resistance and distribution of aminoglycoside resistance genes among Enterococcus spp. clinical isolates in Tehran, IranV. Sharifzadeh Peyvasti0A. Mohabati Mobarez1F. Shahcheraghi2N. Khoramabadi3N. Razaz Rahmati4R. Hosseini Doust5Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran; Corresponding author.Department of Bacteriology, Pasteur Institute of Iran, Tehran, IranDepartment of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Microbiology, Faculty of Advanced Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, IranObjectives: Enterococci have gained attention during the past decade as important nosocomial pathogens. Their increasing prevalence has been paralleled by the occurrence of multidrug-resistant and high-level aminoglycoside-resistant strains. This study isolated Enterococcus spp. from hospital samples and determined their antibiotic resistance profile, focusing on aminoglycosides, and associated resistance mechanisms. Methods: A total of 195 enterococci from hospital samples in Tehran were studied. Isolates were identified by biochemical reactions. Antimicrobial resistance was determined by disk diffusion. The vancomycin MIC for vancomycin-resistant isolates was determined by agar dilution. Detection of aminoglycoside resistance genes and intI1 and intI2 gene was performed by PCR. Results: The majority of isolates were Enterococcus faecalis (65.1%), followed by Enterococcus faecium (31.8%), Enterococcus gallinarum (2.6%) and Enterococcus solitarius (0.5%). According to antibiogram results, 42.1% of isolates were high-level gentamicin-resistant (HLGR) and 40.5% were high-level streptomycin-resistant (HLSR). There was a high prevalence of aac(6')-Ie–aph(2'')-Ia (96.3%) among HLGR isolates. ant(6)-Ia and aadA were identified in 93.7% and 64.6% of HLSR isolates, respectively. aph(2ʹʹ)-Ic was detected in 7 isolates (3.6%) and aph(2ʹʹ)-Ib in only 4 isolates (2.1%); no isolates harboured aph(2ʹʹ)-Id, intI1 or intI2. Conclusion: Multidrug resistance was higher among HLGR and HLSR isolates compared with non-HLGR and non-HLSR isolates, which may result in limited treatment options. More than 50% of isolates were susceptible to aminoglycosides, thus correct identification in clinical laboratories and administration of these antibiotics can result in decreased used of antibiotics such as vancomycin and linezolid and help to reduce the emergence of resistance to these drugs.http://www.sciencedirect.com/science/article/pii/S2213716519302061EnterococcusAminoglycoside resistance geneHigh-level aminoglycoside resistance |
| spellingShingle | V. Sharifzadeh Peyvasti A. Mohabati Mobarez F. Shahcheraghi N. Khoramabadi N. Razaz Rahmati R. Hosseini Doust High-level aminoglycoside resistance and distribution of aminoglycoside resistance genes among Enterococcus spp. clinical isolates in Tehran, Iran Journal of Global Antimicrobial Resistance Enterococcus Aminoglycoside resistance gene High-level aminoglycoside resistance |
| title | High-level aminoglycoside resistance and distribution of aminoglycoside resistance genes among Enterococcus spp. clinical isolates in Tehran, Iran |
| title_full | High-level aminoglycoside resistance and distribution of aminoglycoside resistance genes among Enterococcus spp. clinical isolates in Tehran, Iran |
| title_fullStr | High-level aminoglycoside resistance and distribution of aminoglycoside resistance genes among Enterococcus spp. clinical isolates in Tehran, Iran |
| title_full_unstemmed | High-level aminoglycoside resistance and distribution of aminoglycoside resistance genes among Enterococcus spp. clinical isolates in Tehran, Iran |
| title_short | High-level aminoglycoside resistance and distribution of aminoglycoside resistance genes among Enterococcus spp. clinical isolates in Tehran, Iran |
| title_sort | high level aminoglycoside resistance and distribution of aminoglycoside resistance genes among enterococcus spp clinical isolates in tehran iran |
| topic | Enterococcus Aminoglycoside resistance gene High-level aminoglycoside resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2213716519302061 |
| work_keys_str_mv | AT vsharifzadehpeyvasti highlevelaminoglycosideresistanceanddistributionofaminoglycosideresistancegenesamongenterococcussppclinicalisolatesintehraniran AT amohabatimobarez highlevelaminoglycosideresistanceanddistributionofaminoglycosideresistancegenesamongenterococcussppclinicalisolatesintehraniran AT fshahcheraghi highlevelaminoglycosideresistanceanddistributionofaminoglycosideresistancegenesamongenterococcussppclinicalisolatesintehraniran AT nkhoramabadi highlevelaminoglycosideresistanceanddistributionofaminoglycosideresistancegenesamongenterococcussppclinicalisolatesintehraniran AT nrazazrahmati highlevelaminoglycosideresistanceanddistributionofaminoglycosideresistancegenesamongenterococcussppclinicalisolatesintehraniran AT rhosseinidoust highlevelaminoglycosideresistanceanddistributionofaminoglycosideresistancegenesamongenterococcussppclinicalisolatesintehraniran |