Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019–2021
This study aimed to determine the prevalence of bacterial diseases in pig farms in various regions of Zhejiang Province and surrounding areas. A total of 526 samples were collected from 85 pig farms in Zhejiang Province and surrounding areas. In this study, samples were analyzed using bacterial isol...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/8/502 |
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| author | Xiangfei Xu Junxing Li Pan Huang Xuemei Cui Xuefeng Li Jiaying Sun Yee Huang Quanan Ji Qiang Wei Guolian Bao Yan Liu |
| author_facet | Xiangfei Xu Junxing Li Pan Huang Xuemei Cui Xuefeng Li Jiaying Sun Yee Huang Quanan Ji Qiang Wei Guolian Bao Yan Liu |
| author_sort | Xiangfei Xu |
| collection | DOAJ |
| description | This study aimed to determine the prevalence of bacterial diseases in pig farms in various regions of Zhejiang Province and surrounding areas. A total of 526 samples were collected from 85 pig farms in Zhejiang Province and surrounding areas. In this study, samples were analyzed using bacterial isolation and purification, Gram staining, PCR amplification, and antimicrobial susceptibility testing. A total of 36 <i>Pasteurella multocida</i> (<i>Pm</i>) isolates were detected, with an isolation rate of 6.84%; 37 <i>Bordetella bronchiseptica</i> (<i>Bb</i>) isolates were detected, with an isolation rate of 7.03%; 60 <i>Glasserella parasuis</i> (<i>G. parasuis</i>) isolates were detected, with an isolation rate of 11.41%; 170 <i>Escherichia coli</i> (<i>E. coli</i>) isolates were detected, with an isolation rate of 32.32%; 67 <i>Streptococcus suis</i> (<i>SS</i>) isolates were detected, with an isolation rate of 12.74%; 44 <i>Actinobacillus pleuropneumoniae</i> (<i>APP</i>) isolates were detected, with an isolation rate of 8.37%; and 7 <i>Salmonella enteritis</i> (<i>SE</i>) isolates were detected, with an isolation rate of 1.33%. Antimicrobial drug susceptibility testing against 21 types of antibiotics was carried out on the isolated strains, and the results showed that 228 strains had varying degrees of resistance to 21 antibiotics, including <i>Pm</i>, <i>Bb</i>, <i>E. coli</i>, and <i>APP</i>, with the highest resistance to lincomycin, at 100%. <i>Pm</i> and <i>APP</i> were the most sensitive to cephalothin, with resistance rates of 0. In terms of strains, <i>Pm</i> had the highest overall sensitivity to 21 antibiotics, and <i>E. coli</i> had the highest resistance. In short, bacterial diseases in Zhejiang and the surrounding areas were harmful, and the drug resistance situation was severe. This study provides scientific guidance for the clinical treatment of bacterial diseases. |
| first_indexed | 2024-03-10T23:30:44Z |
| format | Article |
| id | doaj.art-75a9f634fc0e431090f3909311132bd9 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T23:30:44Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-75a9f634fc0e431090f3909311132bd92023-11-19T03:18:51ZengMDPI AGVeterinary Sciences2306-73812023-08-0110850210.3390/vetsci10080502Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019–2021Xiangfei Xu0Junxing Li1Pan Huang2Xuemei Cui3Xuefeng Li4Jiaying Sun5Yee Huang6Quanan Ji7Qiang Wei8Guolian Bao9Yan Liu10Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThis study aimed to determine the prevalence of bacterial diseases in pig farms in various regions of Zhejiang Province and surrounding areas. A total of 526 samples were collected from 85 pig farms in Zhejiang Province and surrounding areas. In this study, samples were analyzed using bacterial isolation and purification, Gram staining, PCR amplification, and antimicrobial susceptibility testing. A total of 36 <i>Pasteurella multocida</i> (<i>Pm</i>) isolates were detected, with an isolation rate of 6.84%; 37 <i>Bordetella bronchiseptica</i> (<i>Bb</i>) isolates were detected, with an isolation rate of 7.03%; 60 <i>Glasserella parasuis</i> (<i>G. parasuis</i>) isolates were detected, with an isolation rate of 11.41%; 170 <i>Escherichia coli</i> (<i>E. coli</i>) isolates were detected, with an isolation rate of 32.32%; 67 <i>Streptococcus suis</i> (<i>SS</i>) isolates were detected, with an isolation rate of 12.74%; 44 <i>Actinobacillus pleuropneumoniae</i> (<i>APP</i>) isolates were detected, with an isolation rate of 8.37%; and 7 <i>Salmonella enteritis</i> (<i>SE</i>) isolates were detected, with an isolation rate of 1.33%. Antimicrobial drug susceptibility testing against 21 types of antibiotics was carried out on the isolated strains, and the results showed that 228 strains had varying degrees of resistance to 21 antibiotics, including <i>Pm</i>, <i>Bb</i>, <i>E. coli</i>, and <i>APP</i>, with the highest resistance to lincomycin, at 100%. <i>Pm</i> and <i>APP</i> were the most sensitive to cephalothin, with resistance rates of 0. In terms of strains, <i>Pm</i> had the highest overall sensitivity to 21 antibiotics, and <i>E. coli</i> had the highest resistance. In short, bacterial diseases in Zhejiang and the surrounding areas were harmful, and the drug resistance situation was severe. This study provides scientific guidance for the clinical treatment of bacterial diseases.https://www.mdpi.com/2306-7381/10/8/502bacterial diseaseisolationidentificationdrug resistance |
| spellingShingle | Xiangfei Xu Junxing Li Pan Huang Xuemei Cui Xuefeng Li Jiaying Sun Yee Huang Quanan Ji Qiang Wei Guolian Bao Yan Liu Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019–2021 Veterinary Sciences bacterial disease isolation identification drug resistance |
| title | Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019–2021 |
| title_full | Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019–2021 |
| title_fullStr | Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019–2021 |
| title_full_unstemmed | Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019–2021 |
| title_short | Isolation, Identification and Drug Resistance Rates of Bacteria from Pigs in Zhejiang and Surrounding Areas during 2019–2021 |
| title_sort | isolation identification and drug resistance rates of bacteria from pigs in zhejiang and surrounding areas during 2019 2021 |
| topic | bacterial disease isolation identification drug resistance |
| url | https://www.mdpi.com/2306-7381/10/8/502 |
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