gjSOX9 Cloning, Expression, and Comparison with gjSOXs Family Members in <i>Gekko japonicus</i>
SOX9 plays a crucial role in the male reproductive system, brain, and kidneys. In this study, we firstly analyzed the complete cDNA sequence and expression patterns for SOX9 from <i>Gekko japonicus</i> SOX9 (gjSOX9), carried out bioinformatic analyses of physiochemical properties, struct...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/11/584 |
| _version_ | 1797459674048495616 |
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| author | Xingze Huang Ruonan Zhao Zhiwang Xu Chuyan Fu Lei Xie Shuran Li Xiaofeng Wang Yongpu Zhang |
| author_facet | Xingze Huang Ruonan Zhao Zhiwang Xu Chuyan Fu Lei Xie Shuran Li Xiaofeng Wang Yongpu Zhang |
| author_sort | Xingze Huang |
| collection | DOAJ |
| description | SOX9 plays a crucial role in the male reproductive system, brain, and kidneys. In this study, we firstly analyzed the complete cDNA sequence and expression patterns for SOX9 from <i>Gekko japonicus</i> SOX9 (gjSOX9), carried out bioinformatic analyses of physiochemical properties, structure, and phylogenetic evolution, and compared these with other members of the gjSOX family. The results indicate that gjSOX9 cDNA comprises 1895 bp with a 1482 bp ORF encoding 494aa. gjSOX9 was not only expressed in various adult tissues but also exhibited a special spatiotemporal expression pattern in gonad tissues. gjSOX9 was predicted to be a hydrophilic nucleoprotein with a characteristic HMG-Box harboring a newly identified unique sequence, “YKYQPRRR”, only present in SOXE members. Among the 20 SOX9 orthologs, gjSOX9 shares the closest genetic relationships with <i>Eublepharis macularius</i> SOX9, <i>Sphacrodactylus townsendi</i> SOX9, and <i>Hemicordylus capensis</i> SOX9. gjSOX9 and gjSOX10 possessed identical physicochemical properties and subcellular locations and were tightly clustered with gjSOX8 in the SOXE group. Sixteen gjSOX family members were divided into six groups: SOXB, C, D, E, F, and H with gjSOX8, 9, and 10 in SOXE among 150 SOX homologs. Collectively, the available data in this study not only facilitate a deep exploration of the functions and molecular regulation mechanisms of the gjSOX9 and gjSOX families in <i>G. japonicus</i> but also contribute to basic research regarding the origin and evolution of SOX9 homologs or even sex-determination mode in reptiles. |
| first_indexed | 2024-03-09T16:54:48Z |
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| language | English |
| last_indexed | 2024-03-09T16:54:48Z |
| publishDate | 2023-11-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj.art-75abf94185e1443d9ae17596f16193772023-11-24T14:36:14ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-11-0145119328934110.3390/cimb45110584gjSOX9 Cloning, Expression, and Comparison with gjSOXs Family Members in <i>Gekko japonicus</i>Xingze Huang0Ruonan Zhao1Zhiwang Xu2Chuyan Fu3Lei Xie4Shuran Li5Xiaofeng Wang6Yongpu Zhang7Department of Biotechnology, Life and Environmental Science College, Wenzhou University, Wenzhou 325003, ChinaDepartment of Bioscience, Life and Environmental Science College, Wenzhou University, Wenzhou 325003, ChinaDepartment of Bioscience, Life and Environmental Science College, Wenzhou University, Wenzhou 325003, ChinaDepartment of Biotechnology, Life and Environmental Science College, Wenzhou University, Wenzhou 325003, ChinaDepartment of Bioscience, Life and Environmental Science College, Wenzhou University, Wenzhou 325003, ChinaDepartment of Bioscience, Life and Environmental Science College, Wenzhou University, Wenzhou 325003, ChinaDepartment of Biotechnology, Life and Environmental Science College, Wenzhou University, Wenzhou 325003, ChinaDepartment of Biotechnology, Life and Environmental Science College, Wenzhou University, Wenzhou 325003, ChinaSOX9 plays a crucial role in the male reproductive system, brain, and kidneys. In this study, we firstly analyzed the complete cDNA sequence and expression patterns for SOX9 from <i>Gekko japonicus</i> SOX9 (gjSOX9), carried out bioinformatic analyses of physiochemical properties, structure, and phylogenetic evolution, and compared these with other members of the gjSOX family. The results indicate that gjSOX9 cDNA comprises 1895 bp with a 1482 bp ORF encoding 494aa. gjSOX9 was not only expressed in various adult tissues but also exhibited a special spatiotemporal expression pattern in gonad tissues. gjSOX9 was predicted to be a hydrophilic nucleoprotein with a characteristic HMG-Box harboring a newly identified unique sequence, “YKYQPRRR”, only present in SOXE members. Among the 20 SOX9 orthologs, gjSOX9 shares the closest genetic relationships with <i>Eublepharis macularius</i> SOX9, <i>Sphacrodactylus townsendi</i> SOX9, and <i>Hemicordylus capensis</i> SOX9. gjSOX9 and gjSOX10 possessed identical physicochemical properties and subcellular locations and were tightly clustered with gjSOX8 in the SOXE group. Sixteen gjSOX family members were divided into six groups: SOXB, C, D, E, F, and H with gjSOX8, 9, and 10 in SOXE among 150 SOX homologs. Collectively, the available data in this study not only facilitate a deep exploration of the functions and molecular regulation mechanisms of the gjSOX9 and gjSOX families in <i>G. japonicus</i> but also contribute to basic research regarding the origin and evolution of SOX9 homologs or even sex-determination mode in reptiles.https://www.mdpi.com/1467-3045/45/11/584<i>Gekko japonicus</i>gjSOX9 cDNAexpressionbioinformatics analysesgjSOX family |
| spellingShingle | Xingze Huang Ruonan Zhao Zhiwang Xu Chuyan Fu Lei Xie Shuran Li Xiaofeng Wang Yongpu Zhang gjSOX9 Cloning, Expression, and Comparison with gjSOXs Family Members in <i>Gekko japonicus</i> Current Issues in Molecular Biology <i>Gekko japonicus</i> gjSOX9 cDNA expression bioinformatics analyses gjSOX family |
| title | gjSOX9 Cloning, Expression, and Comparison with gjSOXs Family Members in <i>Gekko japonicus</i> |
| title_full | gjSOX9 Cloning, Expression, and Comparison with gjSOXs Family Members in <i>Gekko japonicus</i> |
| title_fullStr | gjSOX9 Cloning, Expression, and Comparison with gjSOXs Family Members in <i>Gekko japonicus</i> |
| title_full_unstemmed | gjSOX9 Cloning, Expression, and Comparison with gjSOXs Family Members in <i>Gekko japonicus</i> |
| title_short | gjSOX9 Cloning, Expression, and Comparison with gjSOXs Family Members in <i>Gekko japonicus</i> |
| title_sort | gjsox9 cloning expression and comparison with gjsoxs family members in i gekko japonicus i |
| topic | <i>Gekko japonicus</i> gjSOX9 cDNA expression bioinformatics analyses gjSOX family |
| url | https://www.mdpi.com/1467-3045/45/11/584 |
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