Genome Wide Association (GWAS) Analysis and genomic heritability for parasite resistance and growth in European seabass
There is an increasing demand for the breeding programs to focus on resistance against diseases since the treatments or vaccinations increase production costs and, in some cases, they are not available at all. Most of the studies focus on virus and bacterial diseases, but limited information is avai...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422001740 |
| _version_ | 1818243628006375424 |
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| author | Stavroula Oikonomou Zoi Kazlari Maria Papapetrou Kantham Papanna Leonidas Papaharisis Tereza Manousaki Dimitrios Loukovitis Arkadios Dimitroglou Lefteris Kottaras Evgenia Gourzioti Charalampos Pagonis Andreas Kostandis Costas S. Tsigenopoulos Dimitiros Chatziplis |
| author_facet | Stavroula Oikonomou Zoi Kazlari Maria Papapetrou Kantham Papanna Leonidas Papaharisis Tereza Manousaki Dimitrios Loukovitis Arkadios Dimitroglou Lefteris Kottaras Evgenia Gourzioti Charalampos Pagonis Andreas Kostandis Costas S. Tsigenopoulos Dimitiros Chatziplis |
| author_sort | Stavroula Oikonomou |
| collection | DOAJ |
| description | There is an increasing demand for the breeding programs to focus on resistance against diseases since the treatments or vaccinations increase production costs and, in some cases, they are not available at all. Most of the studies focus on virus and bacterial diseases, but limited information is available for parasitic diseases in European seabass. A sample of infected fish (985) with Lernanthropus kroyeri and their parents were genotyped using the recently developed MedFISH SNP array. The sample was selected (selective genotyping) in order to capture the genetic variation of the resistance against the Lernanthropus kroyeri. Body weight was recorded at different growth stages in the population. The genetic parameters were estimated for body weight and parasite count, using Restricted Estimation Maximum Likelihood (REML) methods utilizing a Genomic Relationship Matrix (GRM). Additionally, univariate GWAS, multitrait GWAS and Regional Heritability Mapping (RHM) were performed in order to identify genomic areas related to host resistance and growth. A moderate genomic heritability was detected for growth at sea cage (0.39) and for weight at 2, 4, 6 months after the fish were transferred to the sea cage (0.46–0.41). A moderate to low genomic correlation between growth (body weight/growth at the sea cage) and parasites was observed (0.36–0.21). GWAS results indicated two putative QTL affecting host resistance to Lernanthropus kroyeri count, each one of them explaining approximately 2% of the phenotypic variation. |
| first_indexed | 2024-12-12T14:04:08Z |
| format | Article |
| id | doaj.art-75ad1d0d2e71454294b37159cc5026a2 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-12T14:04:08Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-75ad1d0d2e71454294b37159cc5026a22022-12-22T00:22:16ZengElsevierAquaculture Reports2352-51342022-06-0124101178Genome Wide Association (GWAS) Analysis and genomic heritability for parasite resistance and growth in European seabassStavroula Oikonomou0Zoi Kazlari1Maria Papapetrou2Kantham Papanna3Leonidas Papaharisis4Tereza Manousaki5Dimitrios Loukovitis6Arkadios Dimitroglou7Lefteris Kottaras8Evgenia Gourzioti9Charalampos Pagonis10Andreas Kostandis11Costas S. Tsigenopoulos12Dimitiros Chatziplis13Laboratory of Agrobiotechnology and Inspection of Agricultural Products, Dept of Agricultural Technology, School of Geotechnical Sciences, International Hellenic University, Alexander Campus, P.O. Box 141, 57 400 Sindos, Thessaloniki, Greece; Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes, Pediados, 715 00 Heraklion, Crete, GreeceLaboratory of Agrobiotechnology and Inspection of Agricultural Products, Dept of Agricultural Technology, School of Geotechnical Sciences, International Hellenic University, Alexander Campus, P.O. Box 141, 57 400 Sindos, Thessaloniki, GreeceLaboratory of Agrobiotechnology and Inspection of Agricultural Products, Dept of Agricultural Technology, School of Geotechnical Sciences, International Hellenic University, Alexander Campus, P.O. Box 141, 57 400 Sindos, Thessaloniki, GreeceNireus Aquaculture SA, 190 02 Paiania, Attica, GreeceNireus Aquaculture SA, 190 02 Paiania, Attica, GreeceInstitute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes, Pediados, 715 00 Heraklion, Crete, GreeceLaboratory of Agrobiotechnology and Inspection of Agricultural Products, Dept of Agricultural Technology, School of Geotechnical Sciences, International Hellenic University, Alexander Campus, P.O. Box 141, 57 400 Sindos, Thessaloniki, Greece; Research Institute of Animal Science, ELGO DIMITRA, 58100 Paralimni, Giannitsa, GreeceNireus Aquaculture SA, 190 02 Paiania, Attica, GreeceNireus Aquaculture SA, 190 02 Paiania, Attica, GreeceNireus Aquaculture SA, 190 02 Paiania, Attica, GreeceNireus Aquaculture SA, 190 02 Paiania, Attica, GreeceNireus Aquaculture SA, 190 02 Paiania, Attica, GreeceInstitute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes, Pediados, 715 00 Heraklion, Crete, Greece; Corresponding author.Laboratory of Agrobiotechnology and Inspection of Agricultural Products, Dept of Agricultural Technology, School of Geotechnical Sciences, International Hellenic University, Alexander Campus, P.O. Box 141, 57 400 Sindos, Thessaloniki, GreeceThere is an increasing demand for the breeding programs to focus on resistance against diseases since the treatments or vaccinations increase production costs and, in some cases, they are not available at all. Most of the studies focus on virus and bacterial diseases, but limited information is available for parasitic diseases in European seabass. A sample of infected fish (985) with Lernanthropus kroyeri and their parents were genotyped using the recently developed MedFISH SNP array. The sample was selected (selective genotyping) in order to capture the genetic variation of the resistance against the Lernanthropus kroyeri. Body weight was recorded at different growth stages in the population. The genetic parameters were estimated for body weight and parasite count, using Restricted Estimation Maximum Likelihood (REML) methods utilizing a Genomic Relationship Matrix (GRM). Additionally, univariate GWAS, multitrait GWAS and Regional Heritability Mapping (RHM) were performed in order to identify genomic areas related to host resistance and growth. A moderate genomic heritability was detected for growth at sea cage (0.39) and for weight at 2, 4, 6 months after the fish were transferred to the sea cage (0.46–0.41). A moderate to low genomic correlation between growth (body weight/growth at the sea cage) and parasites was observed (0.36–0.21). GWAS results indicated two putative QTL affecting host resistance to Lernanthropus kroyeri count, each one of them explaining approximately 2% of the phenotypic variation.http://www.sciencedirect.com/science/article/pii/S2352513422001740European seabass (Dicentrarchus labrax)Lernanthropus kroyeriGrowthBody weightMedFish SNP-arrayGWAS |
| spellingShingle | Stavroula Oikonomou Zoi Kazlari Maria Papapetrou Kantham Papanna Leonidas Papaharisis Tereza Manousaki Dimitrios Loukovitis Arkadios Dimitroglou Lefteris Kottaras Evgenia Gourzioti Charalampos Pagonis Andreas Kostandis Costas S. Tsigenopoulos Dimitiros Chatziplis Genome Wide Association (GWAS) Analysis and genomic heritability for parasite resistance and growth in European seabass Aquaculture Reports European seabass (Dicentrarchus labrax) Lernanthropus kroyeri Growth Body weight MedFish SNP-array GWAS |
| title | Genome Wide Association (GWAS) Analysis and genomic heritability for parasite resistance and growth in European seabass |
| title_full | Genome Wide Association (GWAS) Analysis and genomic heritability for parasite resistance and growth in European seabass |
| title_fullStr | Genome Wide Association (GWAS) Analysis and genomic heritability for parasite resistance and growth in European seabass |
| title_full_unstemmed | Genome Wide Association (GWAS) Analysis and genomic heritability for parasite resistance and growth in European seabass |
| title_short | Genome Wide Association (GWAS) Analysis and genomic heritability for parasite resistance and growth in European seabass |
| title_sort | genome wide association gwas analysis and genomic heritability for parasite resistance and growth in european seabass |
| topic | European seabass (Dicentrarchus labrax) Lernanthropus kroyeri Growth Body weight MedFish SNP-array GWAS |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422001740 |
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