In-vitro evaluation of Indigofera heterantha extracts for antibacterial, antifungal and anthelmintic activities
Abstract Background Multidrug-resistant bacterial strains cause several serious infections that can be fatal, such as Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriaceae (often referred to as ESKAPE pathogens). Si...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Journal of Pharmaceutical Health Care and Sciences |
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| Online Access: | https://doi.org/10.1186/s40780-024-00328-y |
| _version_ | 1827329087089147904 |
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| author | Showkat Ahmad Bhat Mohammed Iqbal Zargar Shahid Ud Din Wani Ishfaq Mohiuddin Mubashir Hussain Masoodi Faiyaz Shakeel Mohammad Ali Seema Mehdi |
| author_facet | Showkat Ahmad Bhat Mohammed Iqbal Zargar Shahid Ud Din Wani Ishfaq Mohiuddin Mubashir Hussain Masoodi Faiyaz Shakeel Mohammad Ali Seema Mehdi |
| author_sort | Showkat Ahmad Bhat |
| collection | DOAJ |
| description | Abstract Background Multidrug-resistant bacterial strains cause several serious infections that can be fatal, such as Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriaceae (often referred to as ESKAPE pathogens). Since ancient times, several indigenous medical systems in India have utilized diverse medicinal plants (approximately 80,000 species) as conventional treatments for a variety of illnesses. A member of the Fabaceae family, also referred to as “Himalayan indigo,” Indigofera heterantha Wall, is well known for its therapeutic properties. Methods The present study investigated the antibacterial, antifungal and antihelmintic properties of the roots, bark, leaves, and flowers of I. heterantha from the Kashmir Himalayas. The effectiveness of the extracts against bacteria, fungi, and earthworms. Three of the tested organisms for bacteria were ESKAPE pathogens, as they are responsible for creating fatal bacterial infections. The antifungal potency of I. heterantha aqueous and methanolic extracts was evaluated using the Agar Well Diffusion Assay. The antihelmintic activity was carried out on an adult Pheretima posthuma Indian earth worm, which shares physiological and anatomical similarities with human intestinal roundworm parasites. Results The methanolic extracts of root and bark have shown prominent activity against all bacterial strains, whereas aqueous extracts of flower, root, and leaves have shown promising activity against Staphylococcus aureus. The aqueous extract demonstrated good activity against S. cerevisiae at a concentration of 200 mg/ml with a zone of inhibition of 16 mm, while the methanolic extract displayed comparable activity against the fungal strains. The remaining two strains, P. crysogenum and A. fumigatus, were only moderately active in response to the extracts. All the extracts have shown anthelmintic activity except aqueous flower. Conclusion These results will pave the way for the bioassay-guided isolation of bioactive constituents that may act as hits for further development as potential antibacterial agents against drug-resistant microbial and helminthic infections. |
| first_indexed | 2024-03-07T15:27:49Z |
| format | Article |
| id | doaj.art-75b26138d3284596b1825c5d627928df |
| institution | Directory Open Access Journal |
| issn | 2055-0294 |
| language | English |
| last_indexed | 2024-03-07T15:27:49Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
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| series | Journal of Pharmaceutical Health Care and Sciences |
| spelling | doaj.art-75b26138d3284596b1825c5d627928df2024-03-05T16:36:44ZengBMCJournal of Pharmaceutical Health Care and Sciences2055-02942024-01-0110111510.1186/s40780-024-00328-yIn-vitro evaluation of Indigofera heterantha extracts for antibacterial, antifungal and anthelmintic activitiesShowkat Ahmad Bhat0Mohammed Iqbal Zargar1Shahid Ud Din Wani2Ishfaq Mohiuddin3Mubashir Hussain Masoodi4Faiyaz Shakeel5Mohammad Ali6Seema Mehdi7Department of Pharmaceutical Sciences, School of Applied Science and Technology, University of KashmirDepartment of Pharmaceutical Sciences, School of Applied Science and Technology, University of KashmirDepartment of Pharmaceutical Sciences, School of Applied Science and Technology, University of KashmirDepartment of Zoology, Annamalai UniversityDepartment of Pharmaceutical Sciences, School of Applied Science and Technology, University of KashmirDepartment of Pharmaceutics, College of Pharmacy, King Saud UniversityDepartment of Pharmacy Practice, East Point College of PharmacyDepartment of Pharmacology, JSS College of PharmacyAbstract Background Multidrug-resistant bacterial strains cause several serious infections that can be fatal, such as Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacteriaceae (often referred to as ESKAPE pathogens). Since ancient times, several indigenous medical systems in India have utilized diverse medicinal plants (approximately 80,000 species) as conventional treatments for a variety of illnesses. A member of the Fabaceae family, also referred to as “Himalayan indigo,” Indigofera heterantha Wall, is well known for its therapeutic properties. Methods The present study investigated the antibacterial, antifungal and antihelmintic properties of the roots, bark, leaves, and flowers of I. heterantha from the Kashmir Himalayas. The effectiveness of the extracts against bacteria, fungi, and earthworms. Three of the tested organisms for bacteria were ESKAPE pathogens, as they are responsible for creating fatal bacterial infections. The antifungal potency of I. heterantha aqueous and methanolic extracts was evaluated using the Agar Well Diffusion Assay. The antihelmintic activity was carried out on an adult Pheretima posthuma Indian earth worm, which shares physiological and anatomical similarities with human intestinal roundworm parasites. Results The methanolic extracts of root and bark have shown prominent activity against all bacterial strains, whereas aqueous extracts of flower, root, and leaves have shown promising activity against Staphylococcus aureus. The aqueous extract demonstrated good activity against S. cerevisiae at a concentration of 200 mg/ml with a zone of inhibition of 16 mm, while the methanolic extract displayed comparable activity against the fungal strains. The remaining two strains, P. crysogenum and A. fumigatus, were only moderately active in response to the extracts. All the extracts have shown anthelmintic activity except aqueous flower. Conclusion These results will pave the way for the bioassay-guided isolation of bioactive constituents that may act as hits for further development as potential antibacterial agents against drug-resistant microbial and helminthic infections.https://doi.org/10.1186/s40780-024-00328-yMicrobial resistanceBioactive compoundsIndigofera HeteranthaAntibacterial activityAntifungal activityAntihelmintic activity |
| spellingShingle | Showkat Ahmad Bhat Mohammed Iqbal Zargar Shahid Ud Din Wani Ishfaq Mohiuddin Mubashir Hussain Masoodi Faiyaz Shakeel Mohammad Ali Seema Mehdi In-vitro evaluation of Indigofera heterantha extracts for antibacterial, antifungal and anthelmintic activities Journal of Pharmaceutical Health Care and Sciences Microbial resistance Bioactive compounds Indigofera Heterantha Antibacterial activity Antifungal activity Antihelmintic activity |
| title | In-vitro evaluation of Indigofera heterantha extracts for antibacterial, antifungal and anthelmintic activities |
| title_full | In-vitro evaluation of Indigofera heterantha extracts for antibacterial, antifungal and anthelmintic activities |
| title_fullStr | In-vitro evaluation of Indigofera heterantha extracts for antibacterial, antifungal and anthelmintic activities |
| title_full_unstemmed | In-vitro evaluation of Indigofera heterantha extracts for antibacterial, antifungal and anthelmintic activities |
| title_short | In-vitro evaluation of Indigofera heterantha extracts for antibacterial, antifungal and anthelmintic activities |
| title_sort | in vitro evaluation of indigofera heterantha extracts for antibacterial antifungal and anthelmintic activities |
| topic | Microbial resistance Bioactive compounds Indigofera Heterantha Antibacterial activity Antifungal activity Antihelmintic activity |
| url | https://doi.org/10.1186/s40780-024-00328-y |
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