Bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishment
Summary: Temperate phages can shape bacterial community dynamics and evolution through lytic and lysogenic life cycles. In response, bacteria that resist phage infection can emerge. This study explores phage-based factors that influence bacterial resistance using a model system of temperate P22 phag...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224008174 |
| _version_ | 1797215895473356800 |
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| author | Hiba Baaziz Rita Makhlouf Michael McClelland Bryan B. Hsu |
| author_facet | Hiba Baaziz Rita Makhlouf Michael McClelland Bryan B. Hsu |
| author_sort | Hiba Baaziz |
| collection | DOAJ |
| description | Summary: Temperate phages can shape bacterial community dynamics and evolution through lytic and lysogenic life cycles. In response, bacteria that resist phage infection can emerge. This study explores phage-based factors that influence bacterial resistance using a model system of temperate P22 phage and Salmonella both inside and outside the mammalian host. Phages that remained functional despite gene deletions had minimal impact on lysogeny and phage resistance except for deletions in the immI region that substantially reduced lysogeny and increased phage resistance to levels comparable to that observed with an obligately lytic P22. This immI deletion does not make the lysogen less competitive but instead increases the frequency of bacterial lysis. Thus, subtle changes in the balance between lysis and lysogeny during the initial stages of infection can significantly influence the extent of phage resistance in the bacterial population. Our work highlights the complex nature of the phage-bacteria-mammalian host triad. |
| first_indexed | 2024-04-24T11:37:20Z |
| format | Article |
| id | doaj.art-75b29a4c638e41a7881e317faa6179b2 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-24T11:37:20Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-75b29a4c638e41a7881e317faa6179b22024-04-10T04:29:22ZengElsevieriScience2589-00422024-04-01274109595Bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishmentHiba Baaziz0Rita Makhlouf1Michael McClelland2Bryan B. Hsu3Department of Biological Sciences, Fralin Life Sciences Institute, Center for Emerging, and Zoonotic, Arthropod-borne Pathogens, Virginia Tech, Blacksburg, VA 24061, USADepartment of Biological Sciences, Fralin Life Sciences Institute, Center for Emerging, and Zoonotic, Arthropod-borne Pathogens, Virginia Tech, Blacksburg, VA 24061, USADepartment of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA, USADepartment of Biological Sciences, Fralin Life Sciences Institute, Center for Emerging, and Zoonotic, Arthropod-borne Pathogens, Virginia Tech, Blacksburg, VA 24061, USA; Corresponding authorSummary: Temperate phages can shape bacterial community dynamics and evolution through lytic and lysogenic life cycles. In response, bacteria that resist phage infection can emerge. This study explores phage-based factors that influence bacterial resistance using a model system of temperate P22 phage and Salmonella both inside and outside the mammalian host. Phages that remained functional despite gene deletions had minimal impact on lysogeny and phage resistance except for deletions in the immI region that substantially reduced lysogeny and increased phage resistance to levels comparable to that observed with an obligately lytic P22. This immI deletion does not make the lysogen less competitive but instead increases the frequency of bacterial lysis. Thus, subtle changes in the balance between lysis and lysogeny during the initial stages of infection can significantly influence the extent of phage resistance in the bacterial population. Our work highlights the complex nature of the phage-bacteria-mammalian host triad.http://www.sciencedirect.com/science/article/pii/S2589004224008174VirologyMicrobiologyViral microbiology |
| spellingShingle | Hiba Baaziz Rita Makhlouf Michael McClelland Bryan B. Hsu Bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishment iScience Virology Microbiology Viral microbiology |
| title | Bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishment |
| title_full | Bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishment |
| title_fullStr | Bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishment |
| title_full_unstemmed | Bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishment |
| title_short | Bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishment |
| title_sort | bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishment |
| topic | Virology Microbiology Viral microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224008174 |
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