LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2
Abstract Background Long non-coding RNAs (lncRNAs) are critical regulators in the initiation and progression of breast cancer. Our study aims to characterize the functions of LINC02086 which few published in breast cancer and decipher the downstream molecular mechanisms. Methods LINC02086 expression...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-023-03245-w |
| _version_ | 1797414937097666560 |
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| author | Xue Han Fan Shi Shujun Guo Yao Li Hongtao Wang Chuanwang Song Shiwu Wu |
| author_facet | Xue Han Fan Shi Shujun Guo Yao Li Hongtao Wang Chuanwang Song Shiwu Wu |
| author_sort | Xue Han |
| collection | DOAJ |
| description | Abstract Background Long non-coding RNAs (lncRNAs) are critical regulators in the initiation and progression of breast cancer. Our study aims to characterize the functions of LINC02086 which few published in breast cancer and decipher the downstream molecular mechanisms. Methods LINC02086 expression is tested in RNA-seq data from GEPIA database, tumor tissue samples from hospital patients and breast cancer cell lines. LINC02086 was silenced or overexpressed by lenti-virus-mediated shRNAs, or pLVX-Puro plasmids. Luciferase reporter assay and RNA pull-down assay were applied to study interactions between LINC02086, miR-6757-5p and ephrin type-A receptor 2 (EPHA2). LINC02086-silencing MCF-7 cells were injected into mice to establish xenograft animal models. Results Using RNA-seq data, tumor tissue samples and breast cancer cells, LINC02086 was consistently found to be up-regulated in breast cancer, and correlated with poorer prognosis. LINC02086 knockdown decreased cell viability, promoted cell apoptosis and suppressed tumor growth. LINC02086 interacted with miR-6757-5p that interacted with EPHA2.LINC02086 expression was negatively correlated with miR-6757-5p expression (r = -0.5698, P < 0.001) but was positively correlated with EPHA2 expression (r = 0.5061, P < 0.001). miR-6757-5p expression was negatively correlated with EPHA2 expression (r = -0.5919, P < 0.001). LINC02086 regulated EPHA2 via miR-6757-5p. miR-6757-5p/EPHA2 axis was a mediator of the effect of LINC02086 on cell viability and apoptosis. Conclusion LINC02086 increases cell viability and decreases apoptotic cells in breast cancer by sponging miR-6757-5p to upregulate EPHA2. This study presents LINC02086/miR-6757-5p/EPHA2 axis as promising therapeutic targets for breast cancer intervention. |
| first_indexed | 2024-03-09T05:41:13Z |
| format | Article |
| id | doaj.art-75b4e29a4af040a4a6a79bd9ffebffc2 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-03-09T05:41:13Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-75b4e29a4af040a4a6a79bd9ffebffc22023-12-03T12:24:55ZengBMCWorld Journal of Surgical Oncology1477-78192023-11-0121111110.1186/s12957-023-03245-wLINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2Xue Han0Fan Shi1Shujun Guo2Yao Li3Hongtao Wang4Chuanwang Song5Shiwu Wu6Department of Immunology, School of Laboratory Medicine, Bengbu Medical CollegeDepartment of Pathology, the First Affiliated Hospital of Bengbu Medical CollegeDepartment of Immunology, School of Laboratory Medicine, Bengbu Medical CollegeDepartment of Immunology, School of Laboratory Medicine, Bengbu Medical CollegeDepartment of Immunology, School of Laboratory Medicine, Bengbu Medical CollegeDepartment of Immunology, School of Laboratory Medicine, Bengbu Medical CollegeDepartment of Pathology, the First Affiliated Hospital of Bengbu Medical CollegeAbstract Background Long non-coding RNAs (lncRNAs) are critical regulators in the initiation and progression of breast cancer. Our study aims to characterize the functions of LINC02086 which few published in breast cancer and decipher the downstream molecular mechanisms. Methods LINC02086 expression is tested in RNA-seq data from GEPIA database, tumor tissue samples from hospital patients and breast cancer cell lines. LINC02086 was silenced or overexpressed by lenti-virus-mediated shRNAs, or pLVX-Puro plasmids. Luciferase reporter assay and RNA pull-down assay were applied to study interactions between LINC02086, miR-6757-5p and ephrin type-A receptor 2 (EPHA2). LINC02086-silencing MCF-7 cells were injected into mice to establish xenograft animal models. Results Using RNA-seq data, tumor tissue samples and breast cancer cells, LINC02086 was consistently found to be up-regulated in breast cancer, and correlated with poorer prognosis. LINC02086 knockdown decreased cell viability, promoted cell apoptosis and suppressed tumor growth. LINC02086 interacted with miR-6757-5p that interacted with EPHA2.LINC02086 expression was negatively correlated with miR-6757-5p expression (r = -0.5698, P < 0.001) but was positively correlated with EPHA2 expression (r = 0.5061, P < 0.001). miR-6757-5p expression was negatively correlated with EPHA2 expression (r = -0.5919, P < 0.001). LINC02086 regulated EPHA2 via miR-6757-5p. miR-6757-5p/EPHA2 axis was a mediator of the effect of LINC02086 on cell viability and apoptosis. Conclusion LINC02086 increases cell viability and decreases apoptotic cells in breast cancer by sponging miR-6757-5p to upregulate EPHA2. This study presents LINC02086/miR-6757-5p/EPHA2 axis as promising therapeutic targets for breast cancer intervention.https://doi.org/10.1186/s12957-023-03245-wlncRNAsmiRNAsEPHA2Luciferase reporter assayRNA pull-down assayBreast cancer |
| spellingShingle | Xue Han Fan Shi Shujun Guo Yao Li Hongtao Wang Chuanwang Song Shiwu Wu LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2 World Journal of Surgical Oncology lncRNAs miRNAs EPHA2 Luciferase reporter assay RNA pull-down assay Breast cancer |
| title | LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2 |
| title_full | LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2 |
| title_fullStr | LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2 |
| title_full_unstemmed | LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2 |
| title_short | LINC02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging miR-6757-5p and up-regulating EPHA2 |
| title_sort | linc02086 promotes cell viability and inhibits cell apoptosis in breast cancer by sponging mir 6757 5p and up regulating epha2 |
| topic | lncRNAs miRNAs EPHA2 Luciferase reporter assay RNA pull-down assay Breast cancer |
| url | https://doi.org/10.1186/s12957-023-03245-w |
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