Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells
Embryonic and induced pluripotent stem cells (IPSCs) derived from mammalian species are valuable tools for modeling human disease, including retinal degenerative eye diseases that result in visual loss. Restoration of vision has focused on transplantation of neural progenitor cells (NPCs) and retin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/417865 |
| _version_ | 1798044982085419008 |
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| author | Laughing Bear Torrez Yukie Perez Jing Yang Nicole Isolde zur Nieden Henry Klassen Chee Gee Liew |
| author_facet | Laughing Bear Torrez Yukie Perez Jing Yang Nicole Isolde zur Nieden Henry Klassen Chee Gee Liew |
| author_sort | Laughing Bear Torrez |
| collection | DOAJ |
| description | Embryonic and induced pluripotent stem cells (IPSCs) derived from mammalian species are valuable tools for modeling human disease, including retinal degenerative eye diseases that result in visual loss. Restoration of vision has focused on transplantation of neural progenitor cells (NPCs) and retinal pigmented epithelium (RPE) to the retina. Here we used transgenic common marmoset (Callithrix jacchus) and human pluripotent stem cells carrying the enhanced green fluorescent protein (eGFP) reporter as a model system for retinal differentiation. Using suspension and subsequent adherent differentiation cultures, we observed spontaneous in vitro differentiation that included NPCs and cells with pigment granules characteristic of differentiated RPE. Retinal cells derived from human and common marmoset pluripotent stem cells provide potentially unlimited cell sources for testing safety and immune compatibility following autologous or allogeneic transplantation using nonhuman primates in early translational applications. |
| first_indexed | 2024-04-11T23:14:19Z |
| format | Article |
| id | doaj.art-75b504b7b9f4470c9691522c3baac741 |
| institution | Directory Open Access Journal |
| issn | 1687-966X 1687-9678 |
| language | English |
| last_indexed | 2024-04-11T23:14:19Z |
| publishDate | 2012-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj.art-75b504b7b9f4470c9691522c3baac7412022-12-22T03:57:42ZengHindawi LimitedStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/417865417865Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem CellsLaughing Bear Torrez0Yukie Perez1Jing Yang2Nicole Isolde zur Nieden3Henry Klassen4Chee Gee Liew5Stem Cell Center, Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, CA 92521, USAStem Cell Center, Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, CA 92521, USAGavin Herbert Eye Institute, Department of Ophthalmology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USAStem Cell Center, Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, CA 92521, USAGavin Herbert Eye Institute, Department of Ophthalmology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USAStem Cell Center, Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, CA 92521, USAEmbryonic and induced pluripotent stem cells (IPSCs) derived from mammalian species are valuable tools for modeling human disease, including retinal degenerative eye diseases that result in visual loss. Restoration of vision has focused on transplantation of neural progenitor cells (NPCs) and retinal pigmented epithelium (RPE) to the retina. Here we used transgenic common marmoset (Callithrix jacchus) and human pluripotent stem cells carrying the enhanced green fluorescent protein (eGFP) reporter as a model system for retinal differentiation. Using suspension and subsequent adherent differentiation cultures, we observed spontaneous in vitro differentiation that included NPCs and cells with pigment granules characteristic of differentiated RPE. Retinal cells derived from human and common marmoset pluripotent stem cells provide potentially unlimited cell sources for testing safety and immune compatibility following autologous or allogeneic transplantation using nonhuman primates in early translational applications.http://dx.doi.org/10.1155/2012/417865 |
| spellingShingle | Laughing Bear Torrez Yukie Perez Jing Yang Nicole Isolde zur Nieden Henry Klassen Chee Gee Liew Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells Stem Cells International |
| title | Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells |
| title_full | Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells |
| title_fullStr | Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells |
| title_full_unstemmed | Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells |
| title_short | Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells |
| title_sort | derivation of neural progenitors and retinal pigment epithelium from common marmoset and human pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2012/417865 |
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