Characteristics of Biofilm-Forming Ability and Antibiotic Resistance of Cutibacterium acnes and Staphylococcus epidermidis from Acne Vulgaris Patients
Kartika Ruchiatan,1,2 Trustia Rizqandaru,1 Panji Respati Satjamanggala,1 Nisrina Tache,1 Adi Imam Cahyadi,3 Andri Rezano,4,5 Hendra Gunawan,1 Eva Krishna Sutedja,1 Reiva Farah Dwiyana,1 Risa Miliawati Nurul Hidayah,1 Pati Aji Achdiat,1 Endang Sutedja,1 Oki Suwarsa,1 Reti Hindritiani1 1Department of...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-09-01
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| Series: | Clinical, Cosmetic and Investigational Dermatology |
| Subjects: | |
| Online Access: | https://www.dovepress.com/characteristics-of-biofilm-forming-ability-and-antibiotic-resistance-o-peer-reviewed-fulltext-article-CCID |
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| author | Ruchiatan K Rizqandaru T Satjamanggala PR Tache N Cahyadi AI Rezano A Gunawan H Sutedja EK Dwiyana RF Hidayah RMN Achdiat PA Sutedja E Suwarsa O Hindritiani R |
| author_facet | Ruchiatan K Rizqandaru T Satjamanggala PR Tache N Cahyadi AI Rezano A Gunawan H Sutedja EK Dwiyana RF Hidayah RMN Achdiat PA Sutedja E Suwarsa O Hindritiani R |
| author_sort | Ruchiatan K |
| collection | DOAJ |
| description | Kartika Ruchiatan,1,2 Trustia Rizqandaru,1 Panji Respati Satjamanggala,1 Nisrina Tache,1 Adi Imam Cahyadi,3 Andri Rezano,4,5 Hendra Gunawan,1 Eva Krishna Sutedja,1 Reiva Farah Dwiyana,1 Risa Miliawati Nurul Hidayah,1 Pati Aji Achdiat,1 Endang Sutedja,1 Oki Suwarsa,1 Reti Hindritiani1 1Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia; 2Doctorate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; 3Division of Microbiology, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; 4Division of Cell Biology, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang, Indonesia; 5Graduate School of Biomedical Sciences Master Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, IndonesiaCorrespondence: Kartika Ruchiatan, Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran - Dr. Hasan Sadikin Hospital, Jl. Pasteur 38, Bandung, West Java, 40161, Indonesia, Tel +62811247932, Email kartika.ruchiatan@unpad.ac.idIntroduction: Acne vulgaris (AV) is a common and chronic disorder of the pilosebaceous unit and has a multifactorial pathology, including activities of Cutibacterium acnes (C. acnes) and Staphylococcus epidermidis (S. epidermidis). Antibiotic resistance has become a major concern in dermatology daily practice, and the ability of biofilm formation by both bacteria is suggested to increase antibiotic resistance in acne.Purpose: Our aim was to analyze the comparison of antibiotic resistance between biofilm-forming (BF) and non-biofilm-forming (NBF) strains of C. acnes and S. epidermidis towards seven antibiotics commonly used for acne.Methods: This is a cross-sectional analytical study involving 60 patients with AV. Samples were obtained from closed comedones on the forehead using the standardized skin surface biopsy (SSSB) method at the Cosmetic Dermatology Clinic Dr. Hasan Sadikin in Bandung, Indonesia. Isolates were cultured and identified before undergoing the biofilm-forming test using the tissue culture plate method. Antibiotic susceptibility testing for each antibiotic was then performed using the disc diffusion method.Results: The incidence of antibiotic resistance to clindamycin in BF and NBF C. acnes isolates was 54.5% (p=1.00), while in BF and NBF S. epidermidis isolates, it was 54.5% and 45.5% respectively (p=0.67). The incidence of antibiotic resistance to erythromycin and azithromycin in BF and NBF C. acnes isolates was 54.5% and 63.6% respectively (p=1.00), whereas for S. epidermidis BF and NBF isolates, it was 54.5% (p=1.00). There was no resistance observed to tetracycline, doxycycline, levofloxacin, and cotrimoxazole in all groups.Conclusion: There were no significant differences in resistance against seven antibiotics between the C. acnes and S. epidermidis in BF and NBF groups. Furthermore, although statistically not significant, some resistances were observed against clindamycin, erythromycin, and azithromycin. Consequently, the use of these three antibiotics should be judiciously regulated.Keywords: acne vulgaris, antibiotic resistance, biofilm, Cutibacterium acnes, Staphylococcus epidermidis |
| first_indexed | 2024-03-12T00:45:08Z |
| format | Article |
| id | doaj.art-75b5d5dfaa8d4a9c9a9739a54a173e66 |
| institution | Directory Open Access Journal |
| issn | 1178-7015 |
| language | English |
| last_indexed | 2024-03-12T00:45:08Z |
| publishDate | 2023-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical, Cosmetic and Investigational Dermatology |
| spelling | doaj.art-75b5d5dfaa8d4a9c9a9739a54a173e662023-09-14T19:09:02ZengDove Medical PressClinical, Cosmetic and Investigational Dermatology1178-70152023-09-01Volume 162457246586572Characteristics of Biofilm-Forming Ability and Antibiotic Resistance of Cutibacterium acnes and Staphylococcus epidermidis from Acne Vulgaris PatientsRuchiatan KRizqandaru TSatjamanggala PRTache NCahyadi AIRezano AGunawan HSutedja EKDwiyana RFHidayah RMNAchdiat PASutedja ESuwarsa OHindritiani RKartika Ruchiatan,1,2 Trustia Rizqandaru,1 Panji Respati Satjamanggala,1 Nisrina Tache,1 Adi Imam Cahyadi,3 Andri Rezano,4,5 Hendra Gunawan,1 Eva Krishna Sutedja,1 Reiva Farah Dwiyana,1 Risa Miliawati Nurul Hidayah,1 Pati Aji Achdiat,1 Endang Sutedja,1 Oki Suwarsa,1 Reti Hindritiani1 1Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia; 2Doctorate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; 3Division of Microbiology, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia; 4Division of Cell Biology, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang, Indonesia; 5Graduate School of Biomedical Sciences Master Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, IndonesiaCorrespondence: Kartika Ruchiatan, Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran - Dr. Hasan Sadikin Hospital, Jl. Pasteur 38, Bandung, West Java, 40161, Indonesia, Tel +62811247932, Email kartika.ruchiatan@unpad.ac.idIntroduction: Acne vulgaris (AV) is a common and chronic disorder of the pilosebaceous unit and has a multifactorial pathology, including activities of Cutibacterium acnes (C. acnes) and Staphylococcus epidermidis (S. epidermidis). Antibiotic resistance has become a major concern in dermatology daily practice, and the ability of biofilm formation by both bacteria is suggested to increase antibiotic resistance in acne.Purpose: Our aim was to analyze the comparison of antibiotic resistance between biofilm-forming (BF) and non-biofilm-forming (NBF) strains of C. acnes and S. epidermidis towards seven antibiotics commonly used for acne.Methods: This is a cross-sectional analytical study involving 60 patients with AV. Samples were obtained from closed comedones on the forehead using the standardized skin surface biopsy (SSSB) method at the Cosmetic Dermatology Clinic Dr. Hasan Sadikin in Bandung, Indonesia. Isolates were cultured and identified before undergoing the biofilm-forming test using the tissue culture plate method. Antibiotic susceptibility testing for each antibiotic was then performed using the disc diffusion method.Results: The incidence of antibiotic resistance to clindamycin in BF and NBF C. acnes isolates was 54.5% (p=1.00), while in BF and NBF S. epidermidis isolates, it was 54.5% and 45.5% respectively (p=0.67). The incidence of antibiotic resistance to erythromycin and azithromycin in BF and NBF C. acnes isolates was 54.5% and 63.6% respectively (p=1.00), whereas for S. epidermidis BF and NBF isolates, it was 54.5% (p=1.00). There was no resistance observed to tetracycline, doxycycline, levofloxacin, and cotrimoxazole in all groups.Conclusion: There were no significant differences in resistance against seven antibiotics between the C. acnes and S. epidermidis in BF and NBF groups. Furthermore, although statistically not significant, some resistances were observed against clindamycin, erythromycin, and azithromycin. Consequently, the use of these three antibiotics should be judiciously regulated.Keywords: acne vulgaris, antibiotic resistance, biofilm, Cutibacterium acnes, Staphylococcus epidermidishttps://www.dovepress.com/characteristics-of-biofilm-forming-ability-and-antibiotic-resistance-o-peer-reviewed-fulltext-article-CCIDacne vulgarisantibiotic resistancebiofilmcutibacterium acnesstaphylococcus epidermidis |
| spellingShingle | Ruchiatan K Rizqandaru T Satjamanggala PR Tache N Cahyadi AI Rezano A Gunawan H Sutedja EK Dwiyana RF Hidayah RMN Achdiat PA Sutedja E Suwarsa O Hindritiani R Characteristics of Biofilm-Forming Ability and Antibiotic Resistance of Cutibacterium acnes and Staphylococcus epidermidis from Acne Vulgaris Patients Clinical, Cosmetic and Investigational Dermatology acne vulgaris antibiotic resistance biofilm cutibacterium acnes staphylococcus epidermidis |
| title | Characteristics of Biofilm-Forming Ability and Antibiotic Resistance of Cutibacterium acnes and Staphylococcus epidermidis from Acne Vulgaris Patients |
| title_full | Characteristics of Biofilm-Forming Ability and Antibiotic Resistance of Cutibacterium acnes and Staphylococcus epidermidis from Acne Vulgaris Patients |
| title_fullStr | Characteristics of Biofilm-Forming Ability and Antibiotic Resistance of Cutibacterium acnes and Staphylococcus epidermidis from Acne Vulgaris Patients |
| title_full_unstemmed | Characteristics of Biofilm-Forming Ability and Antibiotic Resistance of Cutibacterium acnes and Staphylococcus epidermidis from Acne Vulgaris Patients |
| title_short | Characteristics of Biofilm-Forming Ability and Antibiotic Resistance of Cutibacterium acnes and Staphylococcus epidermidis from Acne Vulgaris Patients |
| title_sort | characteristics of biofilm forming ability and antibiotic resistance of cutibacterium acnes and staphylococcus epidermidis from acne vulgaris patients |
| topic | acne vulgaris antibiotic resistance biofilm cutibacterium acnes staphylococcus epidermidis |
| url | https://www.dovepress.com/characteristics-of-biofilm-forming-ability-and-antibiotic-resistance-o-peer-reviewed-fulltext-article-CCID |
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