An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm
Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X- and Y-chromosome bearing sperm. This technolo...
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| Format: | Article |
| Language: | English |
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Veterinary World
2017-05-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.10/May-2017/5.pdf |
| _version_ | 1818726154010361856 |
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| author | Shiv Kumar Yadav Dharmendra Kumar Gangwar Jarnail Singh Chiranjeev Kumar Tikadar V. Vinoth Khanna Sudha Saini Sunny Dholpuria Prabhat Palta Radhey Shyam Manik Manoj Kumar Singh Suresh Kumar Singla |
| author_facet | Shiv Kumar Yadav Dharmendra Kumar Gangwar Jarnail Singh Chiranjeev Kumar Tikadar V. Vinoth Khanna Sudha Saini Sunny Dholpuria Prabhat Palta Radhey Shyam Manik Manoj Kumar Singh Suresh Kumar Singla |
| author_sort | Shiv Kumar Yadav |
| collection | DOAJ |
| description | Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X- and Y-chromosome bearing sperm. This technology is based on the differences in DNA content between these two types of sperm and has been commercialized for bovine sperm. However, this technology still has problems in terms of high economic cost, sperm damage, and lower pregnancy rates compared to unsorted semen. Therefore, an inexpensive, convenient, and non-invasive approach for sperm sexing would be of benefit to agricultural sector. Within this perspective, immunological sperm sexing method is one of the attractive choices to separate X- and Y-chromosome bearing sperm. This article reviews the current knowledge about immunological approaches, viz., H-Y antigen, sex-specific antigens, and differentially expressed proteins for sperm sexing. Moreover, this review also highlighted the different methods for identification of X- and Y-sperm. |
| first_indexed | 2024-12-17T21:53:41Z |
| format | Article |
| id | doaj.art-75b7fca912ff42599de3038bb90e3cb9 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T21:53:41Z |
| publishDate | 2017-05-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-75b7fca912ff42599de3038bb90e3cb92022-12-21T21:31:12ZengVeterinary WorldVeterinary World0972-89882231-09162017-05-0110549850410.14202/vetworld.2017.498-504An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing spermShiv Kumar Yadav0Dharmendra Kumar Gangwar1Jarnail Singh2Chiranjeev Kumar Tikadar3V. Vinoth Khanna4Sudha Saini5Sunny Dholpuria6Prabhat Palta7Radhey Shyam Manik8Manoj Kumar Singh9Suresh Kumar Singla10Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X- and Y-chromosome bearing sperm. This technology is based on the differences in DNA content between these two types of sperm and has been commercialized for bovine sperm. However, this technology still has problems in terms of high economic cost, sperm damage, and lower pregnancy rates compared to unsorted semen. Therefore, an inexpensive, convenient, and non-invasive approach for sperm sexing would be of benefit to agricultural sector. Within this perspective, immunological sperm sexing method is one of the attractive choices to separate X- and Y-chromosome bearing sperm. This article reviews the current knowledge about immunological approaches, viz., H-Y antigen, sex-specific antigens, and differentially expressed proteins for sperm sexing. Moreover, this review also highlighted the different methods for identification of X- and Y-sperm.http://www.veterinaryworld.org/Vol.10/May-2017/5.pdfdifferentially expressed proteinsH-Y antigensex specific proteinssperm identificationsperm sexing |
| spellingShingle | Shiv Kumar Yadav Dharmendra Kumar Gangwar Jarnail Singh Chiranjeev Kumar Tikadar V. Vinoth Khanna Sudha Saini Sunny Dholpuria Prabhat Palta Radhey Shyam Manik Manoj Kumar Singh Suresh Kumar Singla An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm Veterinary World differentially expressed proteins H-Y antigen sex specific proteins sperm identification sperm sexing |
| title | An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm |
| title_full | An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm |
| title_fullStr | An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm |
| title_full_unstemmed | An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm |
| title_short | An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm |
| title_sort | immunological approach of sperm sexing and different methods for identification of x and y chromosome bearing sperm |
| topic | differentially expressed proteins H-Y antigen sex specific proteins sperm identification sperm sexing |
| url | http://www.veterinaryworld.org/Vol.10/May-2017/5.pdf |
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