Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, India
During the present investigation, studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna, West Godavari and East Godavari districts of Andhra Pradesh. Enteric Red Mouth disease or Yersiniosis is a serious infectious bacterial disease, sometimes...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2021-05-01
|
| Series: | Aquaculture and Fisheries |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X2030068X |
| _version_ | 1797204395417403392 |
|---|---|
| author | Shameem Ummey, Ph.D Sharmila Khan, Ph.D P.P.N Vijayakumar, Ph.D Adanki Ramya, M.Sc. Zoology |
| author_facet | Shameem Ummey, Ph.D Sharmila Khan, Ph.D P.P.N Vijayakumar, Ph.D Adanki Ramya, M.Sc. Zoology |
| author_sort | Shameem Ummey, Ph.D |
| collection | DOAJ |
| description | During the present investigation, studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna, West Godavari and East Godavari districts of Andhra Pradesh. Enteric Red Mouth disease or Yersiniosis is a serious infectious bacterial disease, sometimes responsible for causing severe economic loss to fish farming industry. The disease is clinically characterized by symptoms like bleeding at the base of fins, inside and around mouth, bilateral exophthalmia with or without haemorrhage, and per-ocular and peri-oral haemorrhages. In the present study, live or moribund fish showing lesions similar to enteric redmouth disease were collected and the causative agent was isolated by culture techniques. Bacterial identification was carried out by conventional biochemical methods and molecular polymerase chain reaction. DNA extracted from isolated pathogen was subjected to PCR amplification with a pair of Y. ruckeri specific primers. The PCR product was run on 1.5% agarose gel electrophoresis and positive bands with a molecular size of approximately 500bp were detected. It is similar to that given for PCR products with primers specific for Y. ruckeri. |
| first_indexed | 2024-04-24T08:34:33Z |
| format | Article |
| id | doaj.art-75b9622bb40941d98c22b86c71b2859c |
| institution | Directory Open Access Journal |
| issn | 2468-550X |
| language | English |
| last_indexed | 2024-04-24T08:34:33Z |
| publishDate | 2021-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Aquaculture and Fisheries |
| spelling | doaj.art-75b9622bb40941d98c22b86c71b2859c2024-04-16T17:35:11ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2021-05-0163289299Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, IndiaShameem Ummey, Ph.D0Sharmila Khan, Ph.D1P.P.N Vijayakumar, Ph.D2Adanki Ramya, M.Sc. Zoology3Department of Zoology, Andhra University, Visakhapatnam, 530003, India; Corresponding author. Department of Zoology, College of Science and Technology, Andhra University, Waltair Junction, Visakhapatnam, 530003, Andhra Pradesh, India.Department of Zoology, Andhra University, Visakhapatnam, 530003, IndiaAdvanced Analytical Laboratory, Andhra University, Visakhapatnam, 530003, IndiaDepartment of Zoology, Andhra University, Visakhapatnam, 530003, IndiaDuring the present investigation, studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna, West Godavari and East Godavari districts of Andhra Pradesh. Enteric Red Mouth disease or Yersiniosis is a serious infectious bacterial disease, sometimes responsible for causing severe economic loss to fish farming industry. The disease is clinically characterized by symptoms like bleeding at the base of fins, inside and around mouth, bilateral exophthalmia with or without haemorrhage, and per-ocular and peri-oral haemorrhages. In the present study, live or moribund fish showing lesions similar to enteric redmouth disease were collected and the causative agent was isolated by culture techniques. Bacterial identification was carried out by conventional biochemical methods and molecular polymerase chain reaction. DNA extracted from isolated pathogen was subjected to PCR amplification with a pair of Y. ruckeri specific primers. The PCR product was run on 1.5% agarose gel electrophoresis and positive bands with a molecular size of approximately 500bp were detected. It is similar to that given for PCR products with primers specific for Y. ruckeri.http://www.sciencedirect.com/science/article/pii/S2468550X2030068XYersinia ruckeriPCREnteric Red Mouth DiseaseIndian Major CarpsAndhra Pradesh |
| spellingShingle | Shameem Ummey, Ph.D Sharmila Khan, Ph.D P.P.N Vijayakumar, Ph.D Adanki Ramya, M.Sc. Zoology Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, India Aquaculture and Fisheries Yersinia ruckeri PCR Enteric Red Mouth Disease Indian Major Carps Andhra Pradesh |
| title | Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, India |
| title_full | Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, India |
| title_fullStr | Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, India |
| title_full_unstemmed | Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, India |
| title_short | Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, India |
| title_sort | enteric red mouth disease and its causative bacterium yersinia ruckeri in indian major carps from culture ponds in andhra pradesh india |
| topic | Yersinia ruckeri PCR Enteric Red Mouth Disease Indian Major Carps Andhra Pradesh |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X2030068X |
| work_keys_str_mv | AT shameemummeyphd entericredmouthdiseaseanditscausativebacteriumyersiniaruckeriinindianmajorcarpsfromculturepondsinandhrapradeshindia AT sharmilakhanphd entericredmouthdiseaseanditscausativebacteriumyersiniaruckeriinindianmajorcarpsfromculturepondsinandhrapradeshindia AT ppnvijayakumarphd entericredmouthdiseaseanditscausativebacteriumyersiniaruckeriinindianmajorcarpsfromculturepondsinandhrapradeshindia AT adankiramyamsczoology entericredmouthdiseaseanditscausativebacteriumyersiniaruckeriinindianmajorcarpsfromculturepondsinandhrapradeshindia |