Mineral and Carbon Metabolic Adjustments in Nodules of Symbiotically Grown Faba Bean (<i>Vicia faba</i> L.) Varieties in Response to Organic Phosphorus Supplementation
Phosphorus (P) is a major limiting factor for legume and symbiotic nitrogen fixation (SNF). Although overall adaptations of legumes to P supplementation have been extensively studied in connection with inorganic P, little information is currently available regarding nodulation or SNF responses to or...
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MDPI AG
2023-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/22/3888 |
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| author | Frank K. Amoako Saad Sulieman Karl H. Mühling |
| author_facet | Frank K. Amoako Saad Sulieman Karl H. Mühling |
| author_sort | Frank K. Amoako |
| collection | DOAJ |
| description | Phosphorus (P) is a major limiting factor for legume and symbiotic nitrogen fixation (SNF). Although overall adaptations of legumes to P supplementation have been extensively studied in connection with inorganic P, little information is currently available regarding nodulation or SNF responses to organic P (Po) in hydroponics. We investigated the mineral and carbon metabolism of Po-induced nodules of two contrasting faba bean varieties grown hydroponically under inorganic P (Pi), viz., in P-deficient (2 µM KH<sub>2</sub>PO<sub>4,</sub> −Pi), sufficient-P (200 µM KH<sub>2</sub>PO<sub>4</sub>, +Pi), and phytic acid (200 µM, Po) conditions, and were inoculated with <i>Rhizobium leguminosarum</i> bv. <i>viciae</i> 3841 and grown for 30 days. The results consistently reveal similar growth and biomass partitioning patterns between +Pi and Po, with both varying substantially from −Pi. In comparison, +Pi and Po observed equivalent accumulations of overall elemental P concentrations, with both increasing by 114 and 119%, respectively, relative to −Pi. A principal component analysis on metabolites showed a clear separation of the −Pi treatment from the others, with +Pi and Po correlating closely together, highlighting the nonsignificant differences between them. Additionally, the δ<sup>15</sup>N abundance of shoots, roots, and nodules was not significantly different between treatments and varieties and exhibited negative δ<sup>15</sup>N signatures for all tissues. Our study provides a novel perspective on mineral and carbon metabolism and their regulation of the growth, functioning, and reprogramming of nodules upon phytate supply. |
| first_indexed | 2024-03-09T16:30:29Z |
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| language | English |
| last_indexed | 2024-03-09T16:30:29Z |
| publishDate | 2023-11-01 |
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| series | Plants |
| spelling | doaj.art-75bc5292987d4cc98f509b52ebd58c7e2023-11-24T15:02:08ZengMDPI AGPlants2223-77472023-11-011222388810.3390/plants12223888Mineral and Carbon Metabolic Adjustments in Nodules of Symbiotically Grown Faba Bean (<i>Vicia faba</i> L.) Varieties in Response to Organic Phosphorus SupplementationFrank K. Amoako0Saad Sulieman1Karl H. Mühling2Institute of Plant Nutrition and Soil Science, Kiel University, Hermann-Rodewald-Straße 2, 24118 Kiel, GermanyInstitute of Plant Nutrition and Soil Science, Kiel University, Hermann-Rodewald-Straße 2, 24118 Kiel, GermanyInstitute of Plant Nutrition and Soil Science, Kiel University, Hermann-Rodewald-Straße 2, 24118 Kiel, GermanyPhosphorus (P) is a major limiting factor for legume and symbiotic nitrogen fixation (SNF). Although overall adaptations of legumes to P supplementation have been extensively studied in connection with inorganic P, little information is currently available regarding nodulation or SNF responses to organic P (Po) in hydroponics. We investigated the mineral and carbon metabolism of Po-induced nodules of two contrasting faba bean varieties grown hydroponically under inorganic P (Pi), viz., in P-deficient (2 µM KH<sub>2</sub>PO<sub>4,</sub> −Pi), sufficient-P (200 µM KH<sub>2</sub>PO<sub>4</sub>, +Pi), and phytic acid (200 µM, Po) conditions, and were inoculated with <i>Rhizobium leguminosarum</i> bv. <i>viciae</i> 3841 and grown for 30 days. The results consistently reveal similar growth and biomass partitioning patterns between +Pi and Po, with both varying substantially from −Pi. In comparison, +Pi and Po observed equivalent accumulations of overall elemental P concentrations, with both increasing by 114 and 119%, respectively, relative to −Pi. A principal component analysis on metabolites showed a clear separation of the −Pi treatment from the others, with +Pi and Po correlating closely together, highlighting the nonsignificant differences between them. Additionally, the δ<sup>15</sup>N abundance of shoots, roots, and nodules was not significantly different between treatments and varieties and exhibited negative δ<sup>15</sup>N signatures for all tissues. Our study provides a novel perspective on mineral and carbon metabolism and their regulation of the growth, functioning, and reprogramming of nodules upon phytate supply.https://www.mdpi.com/2223-7747/12/22/3888<i>Vicia faba</i>biological nitrogen fixationdinitrogenmetabolitesorganic phosphorusminerals |
| spellingShingle | Frank K. Amoako Saad Sulieman Karl H. Mühling Mineral and Carbon Metabolic Adjustments in Nodules of Symbiotically Grown Faba Bean (<i>Vicia faba</i> L.) Varieties in Response to Organic Phosphorus Supplementation Plants <i>Vicia faba</i> biological nitrogen fixation dinitrogen metabolites organic phosphorus minerals |
| title | Mineral and Carbon Metabolic Adjustments in Nodules of Symbiotically Grown Faba Bean (<i>Vicia faba</i> L.) Varieties in Response to Organic Phosphorus Supplementation |
| title_full | Mineral and Carbon Metabolic Adjustments in Nodules of Symbiotically Grown Faba Bean (<i>Vicia faba</i> L.) Varieties in Response to Organic Phosphorus Supplementation |
| title_fullStr | Mineral and Carbon Metabolic Adjustments in Nodules of Symbiotically Grown Faba Bean (<i>Vicia faba</i> L.) Varieties in Response to Organic Phosphorus Supplementation |
| title_full_unstemmed | Mineral and Carbon Metabolic Adjustments in Nodules of Symbiotically Grown Faba Bean (<i>Vicia faba</i> L.) Varieties in Response to Organic Phosphorus Supplementation |
| title_short | Mineral and Carbon Metabolic Adjustments in Nodules of Symbiotically Grown Faba Bean (<i>Vicia faba</i> L.) Varieties in Response to Organic Phosphorus Supplementation |
| title_sort | mineral and carbon metabolic adjustments in nodules of symbiotically grown faba bean i vicia faba i l varieties in response to organic phosphorus supplementation |
| topic | <i>Vicia faba</i> biological nitrogen fixation dinitrogen metabolites organic phosphorus minerals |
| url | https://www.mdpi.com/2223-7747/12/22/3888 |
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