Organ-specific splice variants of aquaporin water channel AgAQP1 in the malaria vector Anopheles gambiae.
Aquaporin (AQP) water channels are important for water homeostasis in all organisms. Malaria transmission is dependent on Anopheles mosquitoes. Water balance is a major factor influencing mosquito survival, which may indirectly affect pathogen transmission.We obtained full-length mRNA sequences for...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3774814?pdf=render |
| _version_ | 1818271131932557312 |
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| author | Hitoshi Tsujimoto Kun Liu Paul J Linser Peter Agre Jason L Rasgon |
| author_facet | Hitoshi Tsujimoto Kun Liu Paul J Linser Peter Agre Jason L Rasgon |
| author_sort | Hitoshi Tsujimoto |
| collection | DOAJ |
| description | Aquaporin (AQP) water channels are important for water homeostasis in all organisms. Malaria transmission is dependent on Anopheles mosquitoes. Water balance is a major factor influencing mosquito survival, which may indirectly affect pathogen transmission.We obtained full-length mRNA sequences for Anopheles gambiae aquaporin 1 (AgAQP1) and identified two splice variants for the gene. In vitro expression analysis showed that both variants transported water and were inhibited by Hg(2+). One splice variant (AgAQP1A) was exclusively expressed in adult female ovaries indicating a function in mosquito reproduction. The other splice variant (AgAQP1B) was expressed in the midgut, malpighian tubules and the head in adult mosquitoes. Immunolabeling showed that in malpighian tubules, AgAQP1 is expressed in principal cells in the proximal portion and in stellate cells in the distal portion. Moreover, AgAQP1 is expressed in Johnston's organ (the "ear"), which is important for courtship behavior.These results suggest that AgAQP1 may play roles associated with mating (courtship) and reproduction in addition to water homeostasis in this important African malaria vector. |
| first_indexed | 2024-12-12T21:21:18Z |
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| id | doaj.art-75be32327ac54c14bddf596940303b78 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T21:21:18Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-75be32327ac54c14bddf596940303b782022-12-22T00:11:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7588810.1371/journal.pone.0075888Organ-specific splice variants of aquaporin water channel AgAQP1 in the malaria vector Anopheles gambiae.Hitoshi TsujimotoKun LiuPaul J LinserPeter AgreJason L RasgonAquaporin (AQP) water channels are important for water homeostasis in all organisms. Malaria transmission is dependent on Anopheles mosquitoes. Water balance is a major factor influencing mosquito survival, which may indirectly affect pathogen transmission.We obtained full-length mRNA sequences for Anopheles gambiae aquaporin 1 (AgAQP1) and identified two splice variants for the gene. In vitro expression analysis showed that both variants transported water and were inhibited by Hg(2+). One splice variant (AgAQP1A) was exclusively expressed in adult female ovaries indicating a function in mosquito reproduction. The other splice variant (AgAQP1B) was expressed in the midgut, malpighian tubules and the head in adult mosquitoes. Immunolabeling showed that in malpighian tubules, AgAQP1 is expressed in principal cells in the proximal portion and in stellate cells in the distal portion. Moreover, AgAQP1 is expressed in Johnston's organ (the "ear"), which is important for courtship behavior.These results suggest that AgAQP1 may play roles associated with mating (courtship) and reproduction in addition to water homeostasis in this important African malaria vector.http://europepmc.org/articles/PMC3774814?pdf=render |
| spellingShingle | Hitoshi Tsujimoto Kun Liu Paul J Linser Peter Agre Jason L Rasgon Organ-specific splice variants of aquaporin water channel AgAQP1 in the malaria vector Anopheles gambiae. PLoS ONE |
| title | Organ-specific splice variants of aquaporin water channel AgAQP1 in the malaria vector Anopheles gambiae. |
| title_full | Organ-specific splice variants of aquaporin water channel AgAQP1 in the malaria vector Anopheles gambiae. |
| title_fullStr | Organ-specific splice variants of aquaporin water channel AgAQP1 in the malaria vector Anopheles gambiae. |
| title_full_unstemmed | Organ-specific splice variants of aquaporin water channel AgAQP1 in the malaria vector Anopheles gambiae. |
| title_short | Organ-specific splice variants of aquaporin water channel AgAQP1 in the malaria vector Anopheles gambiae. |
| title_sort | organ specific splice variants of aquaporin water channel agaqp1 in the malaria vector anopheles gambiae |
| url | http://europepmc.org/articles/PMC3774814?pdf=render |
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