Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro Model
<i>Background and Objectives</i>: The aim of this study was to compare the effects of low-level laser therapy and continuous microwave diathermy on the growth of Gram-negative and Gram-positive bacteria and to establish their efficacy as an alternative therapeutic modality. <i>Mate...
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MDPI AG
2019-07-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1010-660X/55/7/330 |
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| author | Snehil Dixit Irfan Ahmad Abdulrahim Hakami Kumar Gular Jaya Shanker Tedla Mohammed Abohashrh |
| author_facet | Snehil Dixit Irfan Ahmad Abdulrahim Hakami Kumar Gular Jaya Shanker Tedla Mohammed Abohashrh |
| author_sort | Snehil Dixit |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: The aim of this study was to compare the effects of low-level laser therapy and continuous microwave diathermy on the growth of Gram-negative and Gram-positive bacteria and to establish their efficacy as an alternative therapeutic modality. <i>Materials and methods</i>: Laser fluence of 13 Joules (J)/cm<sup>2</sup>, 18 J/cm<sup>2</sup> and 30 J/cm<sup>2</sup> were used against several bacterial strains. Microwave dosages of 25, 50 and 100 watts (W) were used, respectively. <i>Results</i>: A significant difference between the three groups was observed using repeated analysis of variance (RANOVA) (F value: 0.74, and <i>p</i> value: 0.001). The Greenhouse−Geisser correction (GG) revealed significant results for laser irradiation alone. However, effect size calculation showed effects with microwave diathermy as well as laser fluence. <i>Conclusions</i>: Low-level laser therapy appears to be an effective modality of treatment when compared with continuous microwave diathermy on the Gram-negative and the Gram-positive bacterial strains tested. Microwave diathermy revealed large and medium effects on the bacterial cell counts with dominant effects on Gram-negative strains. |
| first_indexed | 2024-03-12T09:29:55Z |
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| id | doaj.art-75c38b979e0b43498542a869cdc58eee |
| institution | Directory Open Access Journal |
| issn | 1010-660X |
| language | English |
| last_indexed | 2024-03-12T09:29:55Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-75c38b979e0b43498542a869cdc58eee2023-09-02T13:55:31ZengMDPI AGMedicina1010-660X2019-07-0155733010.3390/medicina55070330medicina55070330Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro ModelSnehil Dixit0Irfan Ahmad1Abdulrahim Hakami2Kumar Gular3Jaya Shanker Tedla4Mohammed Abohashrh5Department of Medical Rehabilitation Sciences, College of Applied Medical Sciences, King Khalid University, Abha 62529, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha 62529, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha 62529, Saudi ArabiaDepartment of Medical Rehabilitation Sciences, College of Applied Medical Sciences, King Khalid University, Abha 62529, Saudi ArabiaDepartment of Medical Rehabilitation Sciences, College of Applied Medical Sciences, King Khalid University, Abha 62529, Saudi ArabiaDepartment of Basic Medical Sciences, College of Applied Medical Sciences, King Khalid University, Abha 62529, Saudi Arabia<i>Background and Objectives</i>: The aim of this study was to compare the effects of low-level laser therapy and continuous microwave diathermy on the growth of Gram-negative and Gram-positive bacteria and to establish their efficacy as an alternative therapeutic modality. <i>Materials and methods</i>: Laser fluence of 13 Joules (J)/cm<sup>2</sup>, 18 J/cm<sup>2</sup> and 30 J/cm<sup>2</sup> were used against several bacterial strains. Microwave dosages of 25, 50 and 100 watts (W) were used, respectively. <i>Results</i>: A significant difference between the three groups was observed using repeated analysis of variance (RANOVA) (F value: 0.74, and <i>p</i> value: 0.001). The Greenhouse−Geisser correction (GG) revealed significant results for laser irradiation alone. However, effect size calculation showed effects with microwave diathermy as well as laser fluence. <i>Conclusions</i>: Low-level laser therapy appears to be an effective modality of treatment when compared with continuous microwave diathermy on the Gram-negative and the Gram-positive bacterial strains tested. Microwave diathermy revealed large and medium effects on the bacterial cell counts with dominant effects on Gram-negative strains.https://www.mdpi.com/1010-660X/55/7/330bacterial culturelow-level laser therapygram stainmicrowave diathermyelectrotherapy |
| spellingShingle | Snehil Dixit Irfan Ahmad Abdulrahim Hakami Kumar Gular Jaya Shanker Tedla Mohammed Abohashrh Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro Model Medicina bacterial culture low-level laser therapy gram stain microwave diathermy electrotherapy |
| title | Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro Model |
| title_full | Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro Model |
| title_fullStr | Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro Model |
| title_full_unstemmed | Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro Model |
| title_short | Comparison of Anti-Microbial Effects of Low-Level Laser Irradiation and Microwave Diathermy on Gram-Positive and Gram-Negative Bacteria in an In Vitro Model |
| title_sort | comparison of anti microbial effects of low level laser irradiation and microwave diathermy on gram positive and gram negative bacteria in an in vitro model |
| topic | bacterial culture low-level laser therapy gram stain microwave diathermy electrotherapy |
| url | https://www.mdpi.com/1010-660X/55/7/330 |
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