Assessment of Efficacy of a Novel Crosslinking Protocol with Intracameral Oxygen (Bubble-CXL) in Increasing the Corneal Stiffness Using Atomic Force Microscopy
The environmental oxygen level plays a critical role in corneal crosslinking (CXL), a treatment method to increase corneal biomechanical stability. In this study, we introduce a new CXL method (Bubble-CXL), in which intracameral oxygen serves as an additional oxygen source during eye treatment. The...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/18/3185 |
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| author | Ammar Alkhalde Hannah Seferovic Ali Abri Alvana Simbrunner Peter Hinterdorfer Yoo Jin Oh |
| author_facet | Ammar Alkhalde Hannah Seferovic Ali Abri Alvana Simbrunner Peter Hinterdorfer Yoo Jin Oh |
| author_sort | Ammar Alkhalde |
| collection | DOAJ |
| description | The environmental oxygen level plays a critical role in corneal crosslinking (CXL), a treatment method to increase corneal biomechanical stability. In this study, we introduce a new CXL method (Bubble-CXL), in which intracameral oxygen serves as an additional oxygen source during eye treatment. The efficiency of this new method was compared with the efficiency of the standard CXL method. Three fresh porcine eye pairs were included in this study. One eye of each pair was treated with standard CXL, whereas in the partner eye, intracameral oxygen was injected prior to CXL and removed at the end of the procedure. The Young’s modulus of each cornea was measured using atomic force microscopy. All analyzed corneas treated with intracameral oxygen showed significantly higher Young’s modulus and thus an increased stiffness compared to the cornea of the partner eye treated with the standard protocol. Using intracameral oxygen in CXL therapy may increase crosslinking efficiency and improve biomechanical corneal properties. |
| first_indexed | 2024-03-09T22:58:11Z |
| format | Article |
| id | doaj.art-75c4a14037104fab96b33c5540c675d3 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T22:58:11Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-75c4a14037104fab96b33c5540c675d32023-11-23T18:06:59ZengMDPI AGNanomaterials2079-49912022-09-011218318510.3390/nano12183185Assessment of Efficacy of a Novel Crosslinking Protocol with Intracameral Oxygen (Bubble-CXL) in Increasing the Corneal Stiffness Using Atomic Force MicroscopyAmmar Alkhalde0Hannah Seferovic1Ali Abri2Alvana Simbrunner3Peter Hinterdorfer4Yoo Jin Oh5Department of Ophthalmology, Hospital Wels-Grieskirchen, 4600 Wels, AustriaInstitute of Biophysics, Johannes Kepler University, 4020 Linz, AustriaDepartment of Ophthalmology, Hospital Wels-Grieskirchen, 4600 Wels, AustriaDepartment of Ophthalmology, Hospital Wels-Grieskirchen, 4600 Wels, AustriaInstitute of Biophysics, Johannes Kepler University, 4020 Linz, AustriaInstitute of Biophysics, Johannes Kepler University, 4020 Linz, AustriaThe environmental oxygen level plays a critical role in corneal crosslinking (CXL), a treatment method to increase corneal biomechanical stability. In this study, we introduce a new CXL method (Bubble-CXL), in which intracameral oxygen serves as an additional oxygen source during eye treatment. The efficiency of this new method was compared with the efficiency of the standard CXL method. Three fresh porcine eye pairs were included in this study. One eye of each pair was treated with standard CXL, whereas in the partner eye, intracameral oxygen was injected prior to CXL and removed at the end of the procedure. The Young’s modulus of each cornea was measured using atomic force microscopy. All analyzed corneas treated with intracameral oxygen showed significantly higher Young’s modulus and thus an increased stiffness compared to the cornea of the partner eye treated with the standard protocol. Using intracameral oxygen in CXL therapy may increase crosslinking efficiency and improve biomechanical corneal properties.https://www.mdpi.com/2079-4991/12/18/3185corneacorneal crosslinkingintracameral oxygenatomic force microscopyforce spectroscopymechanical mapping |
| spellingShingle | Ammar Alkhalde Hannah Seferovic Ali Abri Alvana Simbrunner Peter Hinterdorfer Yoo Jin Oh Assessment of Efficacy of a Novel Crosslinking Protocol with Intracameral Oxygen (Bubble-CXL) in Increasing the Corneal Stiffness Using Atomic Force Microscopy Nanomaterials cornea corneal crosslinking intracameral oxygen atomic force microscopy force spectroscopy mechanical mapping |
| title | Assessment of Efficacy of a Novel Crosslinking Protocol with Intracameral Oxygen (Bubble-CXL) in Increasing the Corneal Stiffness Using Atomic Force Microscopy |
| title_full | Assessment of Efficacy of a Novel Crosslinking Protocol with Intracameral Oxygen (Bubble-CXL) in Increasing the Corneal Stiffness Using Atomic Force Microscopy |
| title_fullStr | Assessment of Efficacy of a Novel Crosslinking Protocol with Intracameral Oxygen (Bubble-CXL) in Increasing the Corneal Stiffness Using Atomic Force Microscopy |
| title_full_unstemmed | Assessment of Efficacy of a Novel Crosslinking Protocol with Intracameral Oxygen (Bubble-CXL) in Increasing the Corneal Stiffness Using Atomic Force Microscopy |
| title_short | Assessment of Efficacy of a Novel Crosslinking Protocol with Intracameral Oxygen (Bubble-CXL) in Increasing the Corneal Stiffness Using Atomic Force Microscopy |
| title_sort | assessment of efficacy of a novel crosslinking protocol with intracameral oxygen bubble cxl in increasing the corneal stiffness using atomic force microscopy |
| topic | cornea corneal crosslinking intracameral oxygen atomic force microscopy force spectroscopy mechanical mapping |
| url | https://www.mdpi.com/2079-4991/12/18/3185 |
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